Milton Carlos Kuga

Universidade Estadual Paulista, Ilha Solteira, Estado de Sao Paulo, Brazil

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Publications (26)23.13 Total impact

  • Article: Effect of the root canal final rinse protocols on the debris and smear layer removal and on the push-out strength of an epoxy-based sealer.
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    ABSTRACT: The aim of the present study was to evaluate the efficacy of QMiX, SmearClear, and 17% EDTA for the debris and smear layer removal from the root canal and its effects on the push-out bond strength of an epoxy-based sealer by scanning electron microscopy (SEM). Forty extracted human canines (n = 10) were assigned to the following final rinse protocols: G1-distilled water (control), G2-17% EDTA, G3-SmearClear, and G4-QMiX. The specimens were submitted to a SEM analysis to evaluate the presence of debris and smear layer, respectively, in the apical or cervical segments. In sequence, forty extracted human maxillary canines with the root canals instrumented were divided into four groups (n = 10) similar to the SEM analysis study. After the filling with AH Plus, the roots were transversally sectioned to obtain dentinal slices. The specimens were submitted to a push-out bond strength test using an electromechanical testing machine. The statistical analysis for the SEM and push-out bond strength studies were performed using the Kruskal-Wallis and Dunn tests (α = 5%). There was no difference among the G2, G3, and G4 efficacy in removing the debris and smear layer (P > 0.05). The efficacy of these groups was superior to the control group. The push-out bond strength values of G2, G3, and G4 were superior to the control group. The ability to remove the debris and smear layer by SmearClear and QMiX was as effective as the 17% EDTA. The final rinse with these solutions promoted similar push-out bond strength values. Microsc. Res. Tech., 2013. © 2013 Wiley Periodicals, Inc.
    Microscopy Research and Technique 02/2013; · 1.79 Impact Factor
  • Article: Influence of radiopacifying agents on the solubility, pH and antimicrobial activity of portland cement.
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    ABSTRACT: The aim of this study was to evaluate the interference of the radiopacifiers bismuth oxide (BO), bismuth carbonate (BC), bismuth subnitrate (BS), and zirconiun oxide (ZO) on the solubility, alkalinity and antimicrobial properties of white Portland cement (WPC). The substances were incorporated to PC, at a ratio of 1:4 (v/v) and subjected to a solubility test. To evaluate the pH, the cements were inserted into retrograde cavities prepared in simulated acrylic teeth and immediately immersed in deionized water. The pH of the solution was measured at 3, 24, 72 and 168 h. The antimicrobial activity was evaluated by a radial diffusion method against the microorganisms S. aureus (ATCC 25923), P. aeruginosa (ATCC 27853), E. faecalis (ATCC 29212) and C. albicans (ATCC 10231). The zone of microbial growth inhibition was measured after 24 h. The addition of BS and BC increased the solubility of the cement. The pH values demonstrated that all materials produced alkaline levels. At 3 h, BS showed lower pH than WPC (p<0.05). At 168 h, all materials showed similar pHs (p>0.05). The materials did not present antimicrobial activity for S. aureus, P. aeruginosas and E. faecalis (p>0.05). With regards to C. albicans, all materials formed an inhibition zone, mainly the mixture of WPC with ZO (p<0.05). The type of radiopacifier incorporated into WPC interfered with its physical and antimicrobial properties. ZO was found to be a viable radiopacifier that can be used with WPC.
    Brazilian dental journal 10/2012; 23(5):515-20.
  • Article: Response of mice connective tissue to intracanal dressings containing chlorhexidine.
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    ABSTRACT: Substances containing chlorhexidine (CHX) have been studied as intracanal medicaments. The aim of the present study was to characterize the response of mouse subcutaneous connective tissue to CHX-containing medications by conventional optical microscopy. The tissue response was evaluated by implanting polyethylene tubes containing one of the substances evaluated: Calen paste + 0.5% CHX, Calen + 2% CHX, 2% CHX gel, and Calen paste (control). After experimental periods of 7, 21, and 63 days, the implants (n = 10) were removed along with the subcutaneous connective tissue. Tissue samples were subjected to histological processing, and sections were stained with hematoxylin and eosin. Qualitative and quantitative analyses of the number of inflammatory cells, blood vessels, and vascularized areas were performed. Results were analyzed by ANOVA and Tukey tests with the significance level set at 5%. We concluded that Calen + 0.5% CHX led to reparative tissue response in contrast with Calen + 2% CHX and 2% CHX gel, which induced persistent inflammatory response, pointing to the aggressive nature of this mixture. When Calen + 2% CHX and 2% CHX gel were compared, the latter induced more intense inflammatory response. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.
    Microscopy Research and Technique 08/2012; · 1.79 Impact Factor
  • Article: Persistence of endodontic methacrylate-based cement residues on dentin adhesive surface treated with different chemical removal protocols.
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    ABSTRACT: The aim of this study was to evaluate the persistence of methacrylate-based cement residues on the dentin, after dentin surface cleaning with ethanol or acetone, with or without previous application of a dentin adhesive. Forty bovine crown fragments were obtained and the dentin surface was washed with 1.0 mL of 2.5% sodium hypochlorite (NaOCl), followed by 0.1 mL of 17% ethylenediaminetetraacetic acid application for 3 min, and final irrigation with 2.5% NaOCl. The specimens were air dried and resin-based cement was rubbed onto the dentine surface with a microbrush applicator. In 20 specimens, previously to cement, a dentin adhesive was applied in all surfaces. After 15 min, the surface was scrubbed with a cotton pellet and moistened with ethanol or acetone, compounding the following groups: G1-99.5% ethanol and G2-acetone, without previous use of dentin adhesive; G3-99.5% ethanol and G4-acetone, with previous use of dentin adhesive. The dentin surface was scrubbed until the cement residues could not be visually detected. Sections were then processed for scanning electron microscopy and evaluated at 500× magnification and scores were attributed to each image according to the area covered by residual sealer, and data were subjected to Kruskal-Wallis at 5% significance. The lower residue presence was observed in G3 (P = 0.005). All surface presented cement residues when acetone was used as cleaning solution (P = 0.0005). The cleaning solutions were unable to completely remove the cement residues from both surfaces. The ethanol used after previous application of the dentin adhesive promoted the lower presence of residues. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc.
    Microscopy Research and Technique 06/2012; 75(10):1432-1436. · 1.79 Impact Factor
  • Article: Persistence of Epoxy-Based Sealer Residues in Dentin Treated With Different Chemical Removal Protocols.
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    ABSTRACT: The presence of residual endodontic sealer in the pulp chamber may cause discoloration of the dental crown and interfere with the adhesion of restorative materials. The aim of this study was to compare the efficacy of different solvents in removing residues of an epoxy resin-based sealer (AH Plus) from the dentin walls of the pulp chamber, by scanning electron microscopy (SEM). Forty-four bovine incisor dental crown fragments were treated with 17% EDTA and 2.5% NaOCl. Specimens received a coating of AH Plus and were left undisturbed for 5 min. Then, specimens were divided in four groups (n = 10) and cleaned with one of the following solutions: isopropyl alcohol, 95% ethanol, acetone solution, or amyl acetate solution. Negative controls (n = 2) did not receive AH Plus, while in positive controls (n = 2) the sealer was not removed. AH Plus removal was evaluated by SEM, and a score system was applied. Data were analyzed by Kruskal-Wallis and Dunn tests. None of the solutions tested was able to completely remove AH Plus from the dentin of the pulp chamber. Amyl acetate performed better than 95% ethanol and isopropyl alcohol (p < 0.05), but not better than acetone (p > 0.05) in removing the sealer from dentin. No significant differences were observed between acetone, 95% ethanol, and isopropyl alcohol (p > 0.05). It was concluded that amyl acetate and acetone may be good options for cleaning the pulp chamber after obturation with AH Plus. SCANNING 00:1-5,2012. © 2012 Wiley Periodicals,Inc.
    Scanning 06/2012; · 1.07 Impact Factor
  • Article: Percentage of gutta-percha in mesial canals of mandibular molars obturated by lateral compaction or single cone techniques.
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    ABSTRACT: The aim of this study was to compare the percentage of gutta-percha (PGP) in mesial root canals of mandibular molars obturated with LC (Lateral Compaction) or SC (Single Cone) ProTaper Universal System techniques at different levels of the root. Mesial root canals of 20 human permanent molars with similar anatomical characteristics were instrumented using the ProTaper Universal rotary system technique until the F2 instrument, with 20 canals filled by SC ProTaper Universal technique and 20 canals by the LC technique. The mesial roots were sectioned transversely to 3, 5, and 7 mm from the root apex. Digital images of specimens were obtained at MIC-D digital microscope in increases of 30 to 35X. The gutta-percha area was measured using ImageTool software. Data were analyzed using two-way ANOVA and Bonferroni test (α = 0.05). The SC technique provided greater PGP than the LC technique in the apical third (3 mm) (P < 0.001). In the other thirds (5 and 7 mm) there was no statistical difference between the two techniques regarding the PGP (P > 0.05). There was no statistically significant difference between thirds of the root canal for both techniques (P > 0.05). It was concluded that SC technique provided greater PGP than the LC technique in the apical third of mesial root canals of mandibular molars. There was no difference between the two techniques regarding the PGP in the cervical and middle thirds.
    Microscopy Research and Technique 04/2012; 75(9):1229-32. · 1.79 Impact Factor
  • Article: Persistence of resinous cement residues in dentin treated with different chemical removal protocols.
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    ABSTRACT: The aim of this study was to evaluate the persistence of resin cement residues after dentin surface cleaning with different alcohol-based solutions or an essential oil (eucalyptol). Forty bovine teeth were sectioned in order to expose pulp chamber dentin to be washed with 1.0 mL of 2.5% sodium hypochlorite (NaOCl), followed by 0.1 mL of 17% EDTA application for 3 min, and final irrigation with 2.5% NaOCl. The specimens were air dried and resin-based cement was rubbed onto the dentine surface with a microbrush applicator. After 15 min, the surface was scrubbed with a cotton pellet and moistened with different dentin cleaning solutions, compounding the following groups: G1--95% ethanol, G2--70% ethanol, G3--70% isopropyl alcohol, or G4--eucalyptol. The dentin was scrubbed until the cement residues could not be visually detected. Sections were then processed for SEM and evaluated at × 500 magnification. Scores were attributed to each image according to the area covered by residual sealer, and data were subjected to Kruskal-Wallis at 5% significance. Eucalyptol promoted the most adequate dentin cleaning, although no statistical difference was detected amongst the groups (P > 0.05), except between the eucalyptol and 70% ethanol groups (P < 0.05). All the evaluated dentin cleaning solutions were unable to completely remove the cement residues from the dentin surface.
    Microscopy Research and Technique 03/2012; 75(7):982-5. · 1.79 Impact Factor
  • Article: Efficacy of NiTi rotary instruments in removing calcium hydroxide dressing residues from root canal walls.
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    ABSTRACT: The aim of this study: was to evaluate the efficacy of three rotary instrument systems (K3, ProTaper and Twisted File) in removing calcium hydroxide residues from root canal walls. Thirty-four human mandibular incisors were instrumented with the ProTaper System up to the F2 instrument, irrigated with 2.5% NaOCl followed by 17% EDTA, and filled with a calcium hydroxide intracanal dressing. After 7 days, the calcium hydroxide dressing was removed using the following rotary instruments: G1 - NiTi size 25, 0.06 taper, of the K3 System; G2 - NiTi F2, of the ProTaper System; or G3 - NiTi size 25, 0.06 taper, of the Twisted File System. The teeth were longitudinally grooved on the buccal and lingual root surfaces, split along their long axis, and their apical and cervical canal thirds were evaluated by SEM (×1000). The images were scored and the data were statistically analyzed using the Kruskall Wallis test. None of the instruments removed the calcium hydroxide dressing completely, either in the apical or cervical thirds, and no significant differences were observed among the rotary instruments tested (p > 0.05).
    Brazilian oral research 02/2012; 26(1):19-23.
  • Article: Residues of calcium hydroxide-based intracanal medication associated with different vehicles: a scanning electron microscopy evaluation.
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    ABSTRACT: This study evaluated the presence of residues after removal of calcium hydroxide [Ca(OH)(2) ] associated with different vehicles. Thirty single-rooted teeth were instrumented to a master apical file #25 using 2.5% NaOCl as main irrigant and 17% trisodium EDTA (ethylenediaminetetraacetic acid) as final agent irrigant. Then, the root canals were dressed with Ca(OH)(2) associated with silicone oil (Group 1), 2% chlorhexidine gluconate (Group 2), or propylene glycol (Group 3). After coronal sealing, all teeth were kept in a moist environment at room temperature. After 7 days, the teeth were reopened and medicaments were removed using 5 mL of saline solution and instrumentation with master apical file followed by new irrigation with 5 mL of 2.5% NaOCl. Subsequently, teeth were split longitudinally and assessed by scanning electron microscopy. The wall cleanliness of the cervical and apical thirds of the roots were evaluated and scored by three blinded examiners. Statistical analysis was performed using Kruskal-Wallis and Wilcoxon tests at 5% level of significance. All roots had residues of Ca(OH)(2) on the canal walls. All experimental groups had similar results (P > 0.05) regardless of the third evaluated. There was significant difference between the apical and cervical thirds only in Group 3 (P < 0.05). Association of different vehicles to Ca(OH)(2) does not influence the persistence of residues on the root canal walls.
    Microscopy Research and Technique 01/2012; 75(7):898-902. · 1.79 Impact Factor
  • Article: In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution.
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    ABSTRACT: Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.
    Brazilian dental journal 01/2012; 23(3):192-8.
  • Article: Penetration into dentin of sodium hypochlorite associated with acid solutions.
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    ABSTRACT: The objective of this study was to evaluate the penetration of 2.5% NaOCl associated with 17.0% EDTA, 1.0% citric acid, and 1.0% peracetic acid into dentin tubules. The roots of 44 bovine incisors were cross-sectioned and 5-mm-long fragments were produced from their middle thirds. The specimens were instrumented with ProTaper hand files, stained in crystal violet, then sectioned mesiodistally. The buccal fragments were divided into 4 groups (n = 9) and subjected to 2 consecutive 10-minute immersion periods in one of the following acid solutions combined with 2.5% NaOCl: 17.0% EDTA (group 1), 1.0% citric acid (group 2), and 1.0% peracetic acid (group 3). Nine fragments were immersed in 2.5% NaOCl (group 4). The analysis of the penetration of NaOCl solutions into dentin was performed by measuring the depth of crystal violet stain that was bleached using a steromicroscope under ×50 magnification. Statistical comparisons were carried out by Kruskal-Wallis and Dunn's tests at the 5% significance level. Group 1 showed less penetration into dentin than group 4 (P < .05). No statistically significant differences were observed among groups 2, 3, and 4 (P > .05). Association of NaOCl with acid solutions did not increase its penetration depth into root dentin.
    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 12/2011; 112(6):e155-9. · 1.50 Impact Factor
  • Article: Fracture strength of incisor crowns after intracoronal bleaching with sodium percarbonate.
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    ABSTRACT: To compare the fracture resistance of bovine teeth after intracoronal bleaching with sodium percarbonate (SPC) or sodium perborate (SP) mixed with water or 20% hydrogen peroxide (HP). Fifty extracted bovine teeth were divided into four experimental groups (G1-G4) and one control (n = 10) after endodontic treatment. Following root canal obturation, a glass ionomer barrier was placed at the cemento-enamel junction. After that, the pulp chambers were filled with: G1 - SP with water; G2 - SP with 20% HP; G3 - SPC with water; and G4 - SPC with 20% HP. No bleaching agent was used in the control group. Coronal access cavities were sealed with glass ionomer and specimens were immersed in artificial saliva. The bleaching agents were replaced after 7 days, and teeth were kept in artificial saliva for an additional 7 days, after which the pastes were removed and the coronal access cavities were restored with glass ionomer. Crowns were subjected to compressive load at a cross head speed of 0.5 mm min(-1) applied at 135° to the long axis of the root by an EMIC DL2000 testing machine, until coronal fracture. Data were statistically analysed by anova and Tukey test. No differences in fracture resistance were observed between the experimental groups (P > 0.05). However, all experimental groups presented lower fracture resistance than the control group (P < 0.05).  SPC and SP led to equal reduction on fracture resistance of dental crowns, regardless of being mixed with water or 20% HP.
    Dental Traumatology 11/2011; 28(3):238-42. · 1.20 Impact Factor
  • Article: Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis.
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    ABSTRACT: The objective of this study was to evaluate the antibacterial efficacy of irrigating solutions and their combinations against Enterococcus faecalis. One hundred ten single-rooted human teeth were inoculated with E. faecalis and incubated for 21 days. Teeth were divided according to the irrigant: Group I (GI), 2.5% sodium hypochlorite solution (NaOCl); GII, 2.5% NaOCl + 10% citric acid; GIII, 2.5% NaOCl + apple cider vinegar; GIV, apple cider vinegar; GV, 2% chlorhexidine solution; GVI, 1% peracetic acid; GVII, saline solution. Microbiological samples were taken after root canal preparation and 7 days later. Data were submitted to ANOVA (5%). All solutions promoted reduction of E. faecalis after instrumentation, but bacterial counts were higher in the final sample. GI, GV, and GVI had lower bacterial counts than the other groups. The irrigating solutions may present activity but do not eradicate E. faecalis in the root canal system.
    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 04/2011; 112(3):396-400. · 1.50 Impact Factor
  • Article: Axial displacement of fluid in glass capillary after intracoronary reaction of several bleaching agents
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    ABSTRACT: Objective: To indirectly quantify the release of oxygen after intracoronary chemical reaction, by axial displacement of fluid in glass capillaries arranged in dental crowns. Material and methods: Bovine crowns were accessed, the cervical plug was executed and a glass capillary was placed in the vestibular external face,containing an oily vehicle.Following that, four associations of bleaching were prepared and placed in its interior: G1 – sodium perborate and hydrogen peroxide 3%; G2– sodium perborate and water, G3 – sodium percarbonate and hydrogen peroxide 3%; G4 – sodium percarbonate and distilled water. After being kept for 24 hours, the axial displacement of the fluid was quantified and interpreted as the release of oxygen by the chemical reaction.Results:The results showed a trend of the associations with hydrogen peroxide to promote the release of more oxygen. However, there was no difference between sodium perborate and sodium percarbonate(p > 0.05). Conclusion: The experimental groups showed no differences between them during the 24 hours of chemical reaction.
    RSBO : Revista Sul-brasileira de Odontologia. 01/2010;
  • Article: Evaluation of sealing ability and pH of the apical barrier with Portland white cement added of conventional radiopacifying agents
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    ABSTRACT: Introduction: The Portland cement has shown similar biological properties to calcium hydroxide, but its radiopacity is lower. Therefore,the addition of materials that minimize this deficiency should be considered. Objective: To evaluate the apical sealing ability and pH of a white Portland cement added of several radiopacifying agents. Material and methods: Forty bovine roots with incomplete apices were selected, and the foraminal openings were standardized to PM 720G bur.After the external root sealing, an apical intracanal barrier 10.0 mm thick was executed with the white Portland cement powder, pure or added of a radiopacifying agent (iodoform, zinc oxide or bismuth subnitrate),and distilled water (0.37 mL). The apical roots were immersed 24h in water in humid atmosphere, and after that they were immersed 24h in 2% Rhodamine B, under vacuum. In sequence, the roots were longitudinally sectioned, the root fragments were photographed, the images were digitalized and the apical infiltration was measured by the Image Tool program. The pH solutions were also evaluated, in 24h and 48h and 7 and 30 days. Data were submitted to Anova test.Results:The zinc oxide solution has the lowest apical infiltration in relation to the other groups (p < 0.05).The pH behavior varied during the analysis, and in the period of 24h all groups showed the highest values (p < 0.05).Conclusion: The type of radiopacifying agent used interferes in the sealing ability of the apical barrier, and zinc oxide showed to be the most beneficial one. The pH varies according to the period of analysis,and the highest values were obtained in the first 24h (p < 0.05).
    RSBO : Revista Sul-brasileira de Odontologia. 01/2010;
  • Article: Influence of dentin on the hydrogen ions release in calcium hydroxide paste associated with chlorhexidine
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    ABSTRACT: Objective: To measure the pH of a Ca(OH)2 paste, pure or associated with 0.4% of chlorhexidine digluconate (CHX), in 24h, 7 and 14 days.Methods: Twenty bovine roots were standardized and a root perforation was performed 5 mm from the cervical area, opening an access to the root canal. After external sealing, the canals were filled with a Ca(OH)2 paste, pure or associated with CHX 0.4% (n = 10). The opening was sealed and the roots were immediately immersed in distilled water. The pH was measured in the following times: 24h, 7 and 14 days. Similar conditions were performed using polyethylene tubes, which formed four groups: G1 – Ca(OH)2 in dentin; G2 – Ca(OH)2 + CHX in dentin; G3 – Ca(OH)2 in polyethylene tubes; G4 – Ca(OH)2 + CHX in polyethylene tubes. Results: Except for G4, the pH decreased over time. In the first 24h, G3 presented the higher pHvalue, and there were no differences between G1 and G3, and G3 and G4 (p > 0.05). In 7 days the pH was higher for G1, and no differences were found only between G3 and G4 (p > 0.05). In 14 days the pH was higher for G4, and there were significant differences between G1 and G4, and G2 and G3 (p > 0.05). Conclusion: There was a decrease of pH over time in all groups except for the association Ca(OH)2 + CHX in polyethylene tubes. Dentin presented influence on the final pH of the Ca(OH)2 paste.
    Perspectives in Oral Sciences. 01/2010;
  • Article: Evaluation of pH, solubility and marginal leakage in retrobturation cases using Sealer 26® alone or with iodoform
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    ABSTRACT: Introduction: Sealer 26® cement contains bisphenol epoxy resin associated with calcium hydroxide, presenting smaller radiopacity than other endodontic cements.Aiming to improve this property, iodoform has been added in its composition. However, this addition’s possible changes in physical and chemical properties still need to be studied. Objective: To evaluate the apical sealing ability, solubility, and pH of Sealer 26® alone or with iodoform, at several proportions. Material and methods: Three experimental mixtures of Sealer 26®, alone or with iodoform, were prepared and subjected to solubility test. Additionally, these combinations were inserted into polyethylene tubes and immersed in distilled water, and, their pH was evaluated after 24-h and 7-day periods. Subsequently, forty roots of extracted lower incisors subdivided into four groups of 10 specimens each, were retrograde filled with one of the previously described mixtures and gutta-percha points. The roots were immersed in Rhodamine B, under vacuum, for 72 hours. After this period, the specimens were longitudinally sectioned, root fragments photographed, these images scanned, and apical infiltration measured by Image tool software. The obtained data were subjected to statistical analysis, at a significance level of 5%. Results: Marginal leakage and solubility tests did not show any difference among the experimental groups (p > 0.05). pH analysis was only statistically different at 24-h period and between Sealer 26® alone and 1.1g iodoform group (p < 0.05). Conclusion: The presence of iodoform in Sealer 26®, at the used proportions, did not alter the solubility, apical marginal leakage and pH properties of the original cement.
    RSBO. 01/2010;
  • Article: Calcium hydroxide intracanal dressing removal with different rotary instruments and irrigating solutions: a scanning electron microscopy study.
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    ABSTRACT: This study evaluated the efficacy of 2 types of rotary instruments employed in association with sodium hypochlorite (NaOCl) or EDTA in removing calcium hydroxide (CH) residues from root canals dentin walls. Forty-two mandibular human incisors were instrumented with the ProTaper System up to F2 instrument, irrigated with 2.5% NaOCl followed by 17% EDTA and filled with a CH intracanal dressing. After 7 days, the CH dressing was removed using 4 techniques: NiTi rotary instrument size 25, 0.06 taper (K3 Endo) and irrigation with 17% EDTA (Group 1), NiTi rotary F1 instrument (ProTaper) and irrigation with 17% EDTA (Group 2), NiTi rotary instrument size 25, 0.06 taper and irrigation with 2.5% NaOCl (Group 3) and NiTi rotary F1 instrument and irrigation with 2.5% NaOCl (Group 4). Two roots without intracanal dressing were used as negative controls. Teeth were evaluated by scanning electron microscopy, in the cervical and apical canal thirds. None of the techniques removed the CH dressing completely. In the apical and cervical thirds, F1 instrument was better than instrument size 25, 0.06 taper in removing CH residues (p<0.05), regardless of the final irrigating solution. No difference was found between the irrigating solutions in the groups of F1 instrument and of instrument size 25, 0.06 taper (p>0.05). The negative controls had no CH residues on the dentin walls. In conclusion, the ProTaper F1 instrument was better than K3 Endo instrument size 25, 0.06 taper in the removal of CH intracanal medication, regardless of irrigating solution used.
    Brazilian dental journal 01/2010; 21(4):310-4.
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    Article: Effectiveness of rotary or manual techniques for removing a 6-year-old filling material.
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    ABSTRACT: The aim of this study was to evaluate the effectiveness of manual and rotary instrumentation techniques for removing root fillings after different storage times. Twenty-four canals from palatal roots of human maxillary molars were instrumented and filled with gutta-percha and zinc-oxide eugenol-based sealer (Endofill) , and were stored in saline for 6 years. Non-aged control specimens were treated in the same manner and stored for 1 week. All canals were retreated using hand files or ProTaper Universal NiTi rotary system. Radiographs were taken to determine the amount of remaining material in the canals. The roots were vertically split, the halves were examined with a clinical microscope and the obtained images were digitized. The images were evaluated with AutoCAD software and the percentage of residual material was calculated. Data were analyzed with two-way ANOVA and Tukey's test at 5% significance level. There was no statistically significant differences (p>0.05) between the manual and rotary techniques for filling material removal regardless the ageing effect on endodontic sealers. When only the age of the filling material was analyzed microscopically, non-aged fillings that remained on the middle third of the canals presented a higher percentage of material remaining (p<0.05) compared to the aged sealers and to the other thirds of the roots. The apical third showed a higher percentage of residual filling material in both radiographic and microscopic analysis when compared to the other root thirds. In conclusion, all canals presented residual filling material after endodontic retreatment procedures. Microscopic analysis was more effective than radiographs for detection of residual filling material.
    Brazilian dental journal 01/2010; 21(2):148-52.
  • Article: Evaluation of apical surface roughness after root resection: a scanning electron microscopic study.
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    ABSTRACT: The objective of this study was to compare, by scanning electron microscopy, the smoothness of the resected apical root surface after preparation with high-speed #699 bur, #699 bur plus Shofu finishing and Zekrya bur, and with an erbium:YAG laser. Thirty-nine extracted human premolars were instrumented and obturated and their root apices were resected. The roots were divided into 4 groups according to the root resection method. The teeth were dried, metallized, and analyzed at x400 by scanning electron microscopy. Scores ranging from 0 to 3 were attributed to surface smoothness. The data were analyzed by the Kruskal-Wallis test for overall comparison, and by Dunn's test for individual comparisons. Smoother surfaces were observed for the groups treated with the #699 bur and the #699 bur plus Shofu point finishing, while rougher surfaces were obtained with the laser, which differed significantly from the other 2 methods (P < .05). Treatment with the #699 bur plus Shofu finishing yielded better results in terms of surface smoothness, whereas the laser treatment produced more irregular and rougher surfaces after root resection.
    Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 12/2007; 104(6):e74-6. · 1.50 Impact Factor