T. E. Miller

John Innes Centre, Norwich, England, United Kingdom

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Publications (62)133.08 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Previously reported genomic in situ hybridization protocols failed to differentiate Aegilops uniaristata chromosomes in a polyploid wheat background. Different protocols and hybridization temperatures were tested to optimize the differentiation of N genome A. uniaristata chromosomes from those of the A, B and D genomes of wheat. A combination of pre-hybridization of cytological preparations, pre-annealing of the genomic probe with blocking DNA and hybridization at elevated temperatures proved successful. A genomic in situ hybridization protocol for the differentiation of chromosomes from very closely related genomes in a polyploid background is reported.
    Pakistan Journal of Biological Sciences. 01/2002;
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    ABSTRACT: Six wheat lines with recombination between Aegilops uniaristata chromosome 3N and wheat chromosome 3A were produced. These were characterized in terms of exchange points by RFLP analysis. Chromosome 3N carries an undesirable brittle rachis gene and three of the recombinant lines had lost this character. The results also support previously published evidence of a pericentric inversion in chromosome 3N relative to the wheat homoeologous group 3 chromosomes.
    Heredity 05/2000; 84 ( Pt 4):487-92. · 4.11 Impact Factor
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    ABSTRACT: RFLP analyses were performed on wheat-Aegilops uniaristata Vis. addition and translocation lines to confirm the identity of added N-genome chromosomes. Complete 1N, 3N, 4N, 5N and 7N chromosome additions were identified, while the complete long arm and only part of the short arm was identified for chromosome 2N. There were no wheat-like 4/5 and 4/7 translocations in the Ae. uniaristata chromosomes. Chromosome 3N carried an asymmetric pericentric inversion, and the translocation line was a product of centric fusion between the long arms of chromosomes 3B and 3N. Chromosome-specific RAPD and microsatellite markers were also identified for all the added Ae. uniaristata chromosomes available in this set of addition lines. A new genomic in situ hybridization protocol combining pre-annealing of probe and blocking DNA and prehybridization with blocking DNA was developed to differentiate the very closely related genomes of Ae. uniaristata and wheat. Hybridization sites for the repetitive DNA sequences pAs1, pSc119.2 and pTa71 were identified on the N-genome chromosomes of Ae. uniaristata using the fluorescent in situ hybridization technique. Results showed deviation from the previously published ideogram of this species. A new ideogram, which shows the hybridization sites for the above sequences, was produced in which the chromosomes are arranged according to their homoeologous group.
    Theoretical and Applied Genetics 01/2000; 101(8):1173-1179. · 3.66 Impact Factor
  • Wheat Information Service. 01/2000; 91:15-19.
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    E. Uslu, S. M. Reader, T. E. Miller
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    ABSTRACT: Fluorescent in situ hybridization (FISH) was utilized to study disomic wheat (Triticum aestivum)/Dasypyrum villosum chromosome addition lines to characterize the V genome chromosomes of D. villosum L. Candargy. The V genome specific DNA sequence, pHv62; the rye sequence, pSc119.2, the 18S-5.8S-26S rDNA genes, pTa71, and the 5S rDNA sequence, cP5S, were used as probes. Six of the seven added D. villosum chromosomes hybridized with pHv62. One of these, 3V, was shown to be a Robertsonian translocation with a wheat chromosome. The presence of the seventh addition line chromosome was verified by FISH using total genomic D. villosum DNA as probe. FISH with cP5S detected a site on 5V, whilst pTa71 revealed a single site on the short arm of IV, but pSc119.2 detected sites on all the chromosomes of D. villosum. The pHv62 probe was also used in the application of FISH to a number of other species in the Triticeae, including five species of Aegilops, barley and the tetraploid Dasypyrum breviaristatum. Sites were detected only in Thinopyrum bessarabicum and Secale cereale, and this together with the above evidence for pTa71 and pSc119.2 suggests that D. villosum is phylogenetically closest to Th. bessarabicum and S. cereale, and also is not related to Dasypyrum breviaristatum.
    Hereditas 10/1999; 131(2):129 - 134. · 0.96 Impact Factor
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    ABSTRACT: The cloning of genes for complex traits in polyploid plants that possess large genomes, such as hexaploid wheat, requires an efficient strategy. We present here one such strategy focusing on the homologous pairing suppressor (Ph1) locus of wheat. This locus has been shown to affect both premeiotic and meiotic processes, possibly suggesting a complex control. The strategy combined the identification of lines carrying specific deletions using multiplex PCR screening of fast-neutron irradiated wheat populations with the approach of physically mapping the region in the rice genome equivalent to the deletion to reveal its gene content. As a result, we have located the Ph1 factor controlling the euploid-like level of homologous chromosome pairing to the region between two loci (Xrgc846 and Xpsr150A). These loci are located within 400 kb of each other in the rice genome. By sequencing this region of the rice genome, it should now be possible to define the nature of this factor.
    Genetics 01/1999; 153(4):1909-18. · 4.39 Impact Factor
  • Cereal Research Communications 01/1999; 27:273-79. · 0.55 Impact Factor
  • Third International Symposium on New Genetical Approaches to Crop Improvement., Tando Jam, Pakistan; 01/1999
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    ABSTRACT: Using the genomic in situ hybridization (GISH) technique, one translocation line, seven translocation-addition lines, five translocation plus translocation addition lines and two ditelosomic addition lines were identified in backcross progenies of Triticum aestivum L. -Psathyrostachys juncea (Fisch.) Nevski intergeneric hybrids. No complete P. juncea chromosomes were detected in the 25 lines studied. The results suggest that intact P. juncea chromosomes may be difficult to isolate in a wheat background.
    Euphytica 01/1998; 99(2):85-88. · 1.64 Impact Factor
  • EWAC Newsletter. 01/1998;
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    C N Law, E Suarez, T E Miller, A J Worland
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    ABSTRACT: By using aneuploid lines of wheat in the variety Chinese Spring, each of the homoeologous group 1 chromosomes was found to delay ear emergence. More than one gene per chromosome was probably involved, and, because of the delays in ear emergence, at least one of them may be responsible for producing an inhibitor of flowering. The genes interacted with each other and with vernalization and day length. The genes on chromosome 1A were the most potent and 1B the least. The genes on the group 1 chromosomes may be related to the genes for vernalization and day-length sensitivity found on the homoeologous chromosome 1H of barley. Reciprocal monosomic analyses of three varieties detected allelic variation between homologues of group 1 for ear-emergence time.Keywords: day length, ear-emergence time, flowering inhibition, group 1 chromosomes, vernalization, wheat
    Heredity 12/1997; 80(1):83-91. · 4.11 Impact Factor
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    ABSTRACT: Eleven wheat-Ae. umbellulata recombinant lines involving chromosome 1U, including an important high-molecular-weight glutenin locus, were characterized by protein and RFLP markers. Four 1U-1A recombinants, one 1U-1B recombinant, two 1U deletions with either nullisomy for chromosome 1A or 1B and a 1U ditelosomic addition line were detected, while 3 recombinant lines involved 1U and non-homoeologous wheat chromosomes. Similar recombination events were found in independent lines, and no small segmental translocations of Ae. umbellulata chromatin were detected. Correlation of the markers with physical maps of the wheat-Ae. umbellulata breakpoints obtained using in situ hybridization enabled the marker order to be established on chromosomes 1A, 1B and 1U. The short arm of chromosome 1A probably differs from both 1U and 1B by one inversion. As now being found to be universal in the Triticeae, clustering of the genetical map in the distal physical regions of the group 1 chromosomes was found.
    Theoretical and Applied Genetics 01/1997; 94(2):293-297. · 3.66 Impact Factor
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    ABSTRACT: Salt-affected soil is a major world-wide problem with many hectares of land lost to cultivation each year .To combat this problem, the development and assessment of a novel salt tolerant cereal, tritipyrum, was carried out. This is a hybrid between wheat and Thinopyrum bessarabicum. a very salt-tolerant member of the Triticeae.A range of tritipyrums derived from crosses between tetraploid wheat, Triticum durum, and Th. bessarabicum was produced. Although meiosis in ihe tritipyrums was generally regular, chromosome pairing failure was observed in each of the genotypes. In addition, the level of fertility was relatively low, the fertility of bagged spikes ranging from 29% to 51%. One iritipyrum produced multiple seeds in some florets. Three tritipyrums selected at random performed better than their wheat parents in hydroculture experiments in each of three treatments (150, 200 and 250 mol/ m3 Nacl). The potential for the exploitation of tritipyrum in salt-affected soils is discussed.
    Plant Breeding 01/1997; 116(2):127 - 132. · 1.18 Impact Factor
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    ABSTRACT: Two pairs of rDNA sites, with significantly different signal strength, were detected on two pairs of chromosomes of Pennisetum glaucum and P. violaceum. Both sites were physically located at the distal ends of the short arms of these two chromosome pairs, with the major pair being located on the two shortest chromosomes. Restriction fragment length polymorphism (RFLP) analysis enabled one of the two pairs of rDNA sites, designated Nor-P1, to be mapped on the fifth linkage group in Pennisetum.Keywords: genetic mapping, in situ hybridization, Pennisetum, physical mapping, rDNA
    Heredity. 01/1997; 78(5).
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    ABSTRACT: summaryHigh levels of soil aluminium place serious constraints on wheat production on acidic soils, especially in the tropical areas of Africa and South America. Conventional plant breeding has improved the tolerance of the wheat crop, but available genetic variation is limited. The wild relatives of wheat provide a valuable gene pool for the introduction of further genetic variation. One wild species, Aegilops uniaristata Vis. (2n = 2x = 14,NN), is being utilized as a new source of tolerance. Of the addition lines of individual N genome chromosomes of A. uniaristata to wheat (Triticum aestivum L.) which have been established and characterized, chromosome 3N has been shown to confer tolerance to wheat. The three substitution lines in which 3N replaces the homoeologous wheat chromosomes, 3A, 3B or 3D, have also been produced. Growing plants to maturity in a low pH/high Al hydroponics system confirmed that chromosome 3N conferred tolerance to the substitution lines as well as to the addition line. By manipulating the genetic control of homoeologous chromosome pairing, chromosome 3N is being recombined with its wheat homoeologues in order to introduce a smaller alien segment which carries the gene(s) for tolerance but not the agronomically unacceptable brittle rachis gene also carried on chromosome 3N.
    New Phytologist 01/1997; 137(1):93-98. · 6.74 Impact Factor
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    ABSTRACT: Two alleles, Glu-A1r encoding high-molecular-weight (HMW) glutenin subunits 39+40 and Glu-A1s encoding HMW glutenin subunits 41+42, were introgressed to bread wheat (Triticum aestivum L.) cv. Sicco from two accessions of T. boeoticum Boiss. ssp. thaoudar (A genome species, 2n=2x=14). Alleles at Glu-A1 in current commercial bread wheats encode zero or one subunit, and alleles at the homoeoloci Glu-B1 and Glu-D1 encode a maximum of two subunits; hence the maximum number of subunits found in commercial wheats is five, whereas the lines incorporating Glu-A1r and Glu-A1s carry six. Using near-isogenic lines, the current results demonstrated that the introduction of Glu-A1r resulted in diminished dough stickiness and improved stability during mixing compared with Glu-A1a encoding subunit 1, and a small improvement in gluten strength as shown by the SDS- sedimentation test. The introduction of Glu-A1a also resulted in a small improvement in gluten strength predicted by the SDS-sedimentation test. Thus the alleles are of potential value in breeding programmes designed to improve bread-making quality.
    Euphytica 12/1996; 93(1):19-29. · 1.64 Impact Factor
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    ABSTRACT: Aegilops umbellulata Zhuk. carries genes at Glu-U1 loci that code for a pair of high-molecular-weight glutenin subunits not found in common wheat, Triticum aestivum. Wheat-Ae. umbellulata recombinant lines were produced with the aim of transferring genes coding for glutenin subunits from Ae. umbellulata into wheat with minimal flanking material. We used fluorescent genomic in situ hybridization to evaluate the extent of recombination and to map physically the translocation breakpoints on 11 wheat-Ae. umbellulata recombinant lines. In situ hybridization was able to identify alien material in wheat and showed breakpoints not only near the centromeres but also along chromosome arms. To characterize and identify chromosomes further, including deletions along the 1U chromosome, we used simultaneous multiple target in situ hybridization to localize a tandemly repeated DNA sequence (pSc119.2) and the 18S-25S and 5S rRNA genes. One line contained an Ae. umbellulata telocentric chromosome and another two had different terminal deletions, mostly with some wheat chromosome rearrangements. Although from six independent original crosses, the other eight lines included only two types of intercalary wheat-Ae. umbellulata recombination events. Five occurred at the 5S rRNA genes on the short arm of the Ae. umbellulata chromosome with a distal wheat-origin segment, and three breakpoints were proximal to the centromere in the long arm, so most of the long arm was of Ae. umbellulata origin. The results allow characterization of recombination events in the context of the karyotype. They also facilitate the design of crossing programmes to generate lines where smaller Ae. umbellulata chromosome segments are transferred to wheat with the potential to improve bread-making quality by incorporating novel glutenin subunits without undesirable linked genes.
    Theoretical and Applied Genetics 10/1996; 93(5-6):816-25. · 3.66 Impact Factor
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    ABSTRACT: Genetic maps of the homoeologous group-6 chromosomes of bread wheat, Triticum aestivum, have been constructed spanning 103 cM on 6A, 90 cM on 6B and 124 cM on 6D. These maps were transferred to a Chinese Spring (CS) x line #31 cross to locate a dominant powdery mildew resistance gene, Pm12, introgressed into line #31 from Aegilops speltoides. Pm12 was shown to lie on the short arm of translocation chromosome 6BS-6SS.6SL in line #31, but could not be mapped more precisely due to the lack of recombination between the 6S Ae. speltoides segment and chromosome 6B. Possible strategies to reduce the size of the alien segment, which probably encompasses the complete long arm and more than 82% of the short arm of chromosome 6B, are discussed.
    Theoretical and Applied Genetics 04/1996; 92(5):559-65. · 3.66 Impact Factor
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    ABSTRACT: Lines of Triticum aestivum cv. Chinese Spring carrying an additional chromosome 5Eb from Thinopyrum bessarabicum or having chromosome 5A or 5D replaced by chromosome 5Eb were screened in hydroculture for tolerance to salt. The previously reported tolerance of the 5Eb addition line was confirmed and the two substitution lines were shown to have a higher level of survival in 175 mol/m3 NaCl than both the addition and the ‘Chinese Spring’ parent. Reasons for the better tolerance of the substitutions are discussed.
    Plant Breeding 01/1996; 115(1):77-78. · 1.18 Impact Factor
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    ABSTRACT: Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4Sl from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4Sl L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
    Euphytica 01/1996; 89(1):103-106. · 1.64 Impact Factor

Publication Stats

994 Citations
133.08 Total Impact Points

Institutions

  • 1994–2000
    • John Innes Centre
      • Department of Crop Genetics
      Norwich, England, United Kingdom
  • 1996
    • National University of Central Buenos Aires
      Тандиль, Buenos Aires, Argentina
  • 1995
    • University of Reading
      Reading, England, United Kingdom
  • 1991
    • Donald Danforth Plant Science Center
      San Luis, Missouri, United States
  • 1987
    • Max Planck Institute for Plant Breeding Research
      Köln, North Rhine-Westphalia, Germany