Marlene Brandes

Publications (2)15.69 Total impact

• Article: Human thymus exports naive CD8 T cells that can home to nonlymphoid tissues.

  Alfred Zippelius, Gilles Bioley, Frédérique-Anne Le Gal, Nathalie Rufer, Marlene Brandes, Pascal Batard, Magda De Smedt, Jean Plum, Daniel E Speiser, Jean-Charles Cerottini, Pierre-Yves Dietrich, Pedro Romero, Mikaël J Pittet
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  ABSTRACT: Functionally naive CD8 T cells in peripheral blood from adult humans can be fully described by their CD45RA(bright)CCR7(+)CD62L(+) cell surface phenotype. Cord blood lymphocytes, from healthy newborns, are homogenously functionally naive. Accordingly, the majority of cord blood CD8 T cells express the same pattern of cell surface molecules. Unexpectedly, however, a significant fraction of cord blood CD8 T cells express neither CCR7 nor CD62L. Yet these cells remain functionally naive as they contain high levels of TCR excision circles, have long telomeres, display highly polyclonal TCRs, and do not exhibit immediate effector functions. In addition, these CD8 T cells already represent a significant fraction of the mature naive CD8 single-positive thymocyte repertoire and may selectively express the cutaneous lymphocyte Ag. We suggest that CD8 single-positive thymocytes comprise two pools of naive precursors that exhibit distinct homing properties. Once seeded in the periphery, naive CCR7(+)CD62L(+) CD8 T cells patrol secondary lymphoid organs, whereas naive CCR7(-)CD62L(-) CD8 T cells selectively migrate to peripheral tissues such as skin.
  The Journal of Immunology 04/2004; 172(5):2773-7. · 5.79 Impact Factor
• Article: Flexible migration program regulates γδ T-cell involvement in humoral immunity.

  Marlene Brandes, Katharina Willimann, Alois B Lang, Ki-Hoan Nam, Chenggang Jin, Michael B Brenner, Craig T Morita, Bernhard Moser
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  ABSTRACT: gamma delta T cells are inadequately defined both in terms of their migration potential and contribution to antimicrobial immunity. Here, we have examined the migration profile of human blood gamma delta T cells and related cell lines and correlated these findings with their distribution in secondary lymphoid tissues and their function in B-cell cocultures. We find that resting gamma delta T cells are characterized by an inflammatory migration program similar to cells of the innate immune system. However, T-cell receptor (TCR) triggering resulted in the rapid but transient induction of a lymph node (LN)-homing program, as evidenced by functional CCR7 expression and concomitant reduction in expression and function of CCR5 and, to a lesser degree, CCR2. Moreover, the LN-homing program was reflected by the presence of gamma delta T cells in gastrointestinal lymphoid tissues, notably in clusters within germinal centers of B-cell follicles. In line with these findings, V gamma V delta-TCR triggering resulted in prominent expression of essential B-cell costimulatory molecules, including CD40L, OX40, CD70, and ICOS. Furthermore, gamma delta T cells were shown to provide potent B-cell help during in vitro antibody production. Collectively, our findings agree with a role for gamma delta T cells in humoral immunity during the early phase of antimicrobial responses.
  Blood 12/2003; 102(10):3693-701. · 9.90 Impact Factor