M Poornima

Publications (8)13.16 Total impact

• Article: Comparative efficacy of double-stranded RNAs targeting WSSV structural and nonstructural genes in controlling viral multiplication in Penaeus monodon.

  M Sanjuktha, V Stalin Raj, K Aravindan, S V Alavandi, M Poornima, T C Santiago
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  ABSTRACT: RNA interference (RNAi) is a potential strategy to control shrimp viral diseases, including the white spot disease caused by White Spot Syndrome Virus (WSSV). Selection of genes for targeting is an important criterion. We have compared the efficacy of dsRNAs targeting structural (vp28 and vp281) and nonstructural genes (rr1 and dnapol) of WSSV in controlling viral multiplication in Penaeus monodon. Targeting the rr1 and vp28 genes provided better protection (93.3% and 90% survival respectively) compared to vp281 and dnapol in experimentally infected shrimp. Temporal transcriptional analysis of the corresponding genes and PCR-based diagnosis of WSSV in samples collected at different time points in the experiment supported this observation, thereby indicating that targeting a combination of rr1 and vp28 would be effective in limiting WSSV multiplication.
  Archives of Virology 02/2012; 157(5):993-8. · 2.11 Impact Factor
• Article: Supra dietary levels of vitamins C and E enhance antibody production and immune memory in juvenile milkfish, Chanos chanos (Forsskal) to formalin-killed Vibrio vulnificus.

  I S Azad, J Syama Dayal, M Poornima, S A Ali
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  ABSTRACT: Juveniles of milkfish, Chanos chanos (Forsskal), were fed two independent supra dietary levels of vitamins C (500 and 1500 mg kg(-1) feed, T1 and T2) and E (50 and 150 mg kg(-1), T3 and T4). Milkfish fed diets with supra (in addition to the vitamins present in the control diet) and normal levels (T5 containing 90 and 1.2mg of vitamins C and E, respectively, kg(-1) of feed) of vitamins were immunized (ip) with formalin-killed Vibrio vulnificus (FKVV). Priming and booster antibody responses to the injected bacterin were significantly (P<0.05) better in the milkfish juveniles fed supra dietary levels. Survival response of the experimental fish fed supra dietary levels of vitamins (T1, T2 and T3) was significantly (P<0.01) better than that of the control set. Protective response against virulent bacterial challenge of the vaccinated fish fed vitamin-supplemented diets (T2 and T3) was better than the control (T5) and T1 and T4. Memory factor reflecting immunological memory was superior in the fish fed vitamin-supplemented diets. Diets supplemented with either 1500 mg of Vitamin C or 50mg of Vitamin E kg(-1) produced the best antibody responses, final survival and protective response upon challenge. No conclusive inferences could be drawn on the growth responses from the experiment.
  Fish &amp Shellfish Immunology 08/2007; 23(1):154-63. · 3.32 Impact Factor
• Article: Supra dietary levels of vitamins C & E enhance antibody production and immune memory in juvenile milkfish, Chanos chanos (Forsskal) to formalin-killed Vibrio vulnificus.

  I.S Azad, Syamadayal, M. Poornima, S.A. Ali
  Fish and Shellfish Immunology. 01/2006; 23:154-163.
• Source

  Available from: int-res.com

  Article: Nodavirus infection causes mortalities in hatchery produced larvae of Lates calcarifer: first report from India.

  I S Azad, M S Shekhar, A R Thirunavukkarasu, M Poornima, M Kailasam, J J S Rajan, S A Ali, M Abraham, P Ravichandran
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  ABSTRACT: Larvae (15 to 21 d post hatch, dph) of the Asian sea bass Lates calcarifer (Bloch) suffered heavy mortalities (60 to 90%) during the hatchery-rearing phase. Darkened and moribund larvae showed no evidence of bacterial or parasitic infections. Tissue sections of brain and spinal cord showed clear necrotic vacuolation. Electron microscopy revealed membrane-bound viral particles in the cytoplasm of the nerve cells. The viral particles measured 28 to 30 nm in diameter. Primer sets, designed for the amplification of the RNA2 segment of the piscine nodavirus coat protein gene, were used in the RT-PCR analysis of moribund larvae of 20 and 21 dph which produced the amplified product of 430 bp. The clinical manifestations, pathology and electron microscopy observations supported by the RT-PCR analysis suggest that the nerve necrosis was due to nodavirus infection in the larvae. This is the first report of piscine nodavirus infection from the Indian sub-continent.
  Diseases of Aquatic Organisms 03/2005; 63(2-3):113-8. · 2.20 Impact Factor
• Article: Nodavirus infection causes mass mortalities in hatchery produced larvae of Asian seabass, Lates calcarifer, Bloch: first report from India.

  I.S. Azad, M.S. Shekhar, A.R. Thirunavukkarasu, M. Poornima, M. Kailasam, Rajan, J.J.S., S.A. Ali, Mathew Abraham, P. Ravichandran
  Diseases of Aquatic Organisms 01/2005; 63:113-118. · 2.20 Impact Factor
• Source

  Available from: ciba.res.in

  Article: Evaluation of Pseudomonas sp. PM 11 and Vibrio fluvialis PM 17 on immune indices of tiger shrimp, Penaeus monodon.

  S V Alavandi, K K Vijayan, T C Santiago, M Poornima, K P Jithendran, S A Ali, J J S Rajan
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  ABSTRACT: Occurrence of widespread epizootics among cultured stock of shrimp has put research programmes on preventive approaches such as application of probiotics on a high priority in aquaculture. In the present study two bacteria, Pseudomonas sp. PM 11 and Vibrio fluvialis PM 17 were selected as candidate probionts from a pool of bacteria isolated from gut of farm reared sub-adult shrimp and tested for their effect on the immunity indicators of tiger shrimp. Sub-adult shrimp, weighing 14 to 22 g were treated in separate experiments with Pseudomonas sp. PM 11 and V. fluvialis PM 17 at 10(3) bacterial cells ml(-1) in the experimental shrimp culture tanks. One set of experimental animals was treated every 3 days and another set of animals every 7 days with each of the candidate probionts. Estimation of immunological indicators such as haemocyte counts, phenol oxidase and antibacterial activity showed declining trends. The haemocyte counts dropped from 31 x 10(3) to 65 x 10(3) ml(-1) on the first day to 4-16 x 10(3) ml(-1) on the 45th day. Similarly, the phenol oxidase activity declined from 12-32 units on the first day to 11-14 units on 45th day of the experiment. Antibacterial activity of haemolymph reduced to 46-67 percent on the 45th day of the experiment. The results of the study suggest that, the criteria used for the selection of putative probiotic strains in the present study, such as predominant growth on primary isolation media, ability to produce extracellular enzymes and siderophores, did not bring about the desired effect in vivo and improve the immune system in shrimp. Hence, new protocols have to be evolved for selection of microbe(s) as putative probiotics and that, detailed understanding of proven probiotics, employed presently on empirical basis may provide a clue on the selection procedure.
  Fish &amp Shellfish Immunology 09/2004; 17(2):115-20. · 3.32 Impact Factor
• Source

  Available from: icar.org.in

  Article: Diseases of mud crabs (Scylla spp.): an overview

  K P Jithendran, M Poornima, C P Balasubramanian, S Kulasekarapandian
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  ABSTRACT: Mud crabs (Scylla spp.) are the most promising group for diversification of coastal aquaculture. However, the health management vis-a-vis diseases of mud crabs attracted less attention of researchers, and therefore, there is distinct lack of description of their pathogens as compared to other commercially exploited crustacean and finfish species. Culture of mud crab is largely extensive with extremely low input; hence diseases with potential economic impact have not been widely observed and caused problems. However, intensification of mud crab culture is likely to result in occurrences of known or previously unreported or even exotic diseases. Many new pathogens and diseases are being reported with significant pathogenicity and adverse effect on the production of mud crabs across the world. In the present review, the viral, bacterial, fungal, protozoan and metazoan pathogens of mud crabs and their impact on the health of individuals and aquaculture are discussed.
• Article: Supra dietary levels of vitamins C and E enhance antibody production and immune memory in juvenile milkfish, Chanos chanos (Forsskal) to formalin-killed Vibrio vulnificus

  I.S. Azad, J. Syama Dayal, M. Poornima, S.A. Ali
  [show abstract] [hide abstract]
  ABSTRACT: Juveniles of milkfish, Chanos chanos (Forsskal), were fed two independent supra dietary levels of vitamins C (500 and 1500 mg kg−1 feed, T1 and T2) and E (50 and 150 mg kg−1, T3 and T4). Milkfish fed diets with supra (in addition to the vitamins present in the control diet) and normal levels (T5 containing 90 and 1.2 mg of vitamins C and E, respectively, kg−1 of feed) of vitamins were immunized (ip) with formalin-killed Vibrio vulnificus (FKVV). Priming and booster antibody responses to the injected bacterin were significantly (P < 0.05) better in the milkfish juveniles fed supra dietary levels. Survival response of the experimental fish fed supra dietary levels of vitamins (T1, T2 and T3) was significantly (P < 0.01) better than that of the control set. Protective response against virulent bacterial challenge of the vaccinated fish fed vitamin-supplemented diets (T2 and T3) was better than the control (T5) and T1 and T4. Memory factor reflecting immunological memory was superior in the fish fed vitamin-supplemented diets. Diets supplemented with either 1500 mg of Vitamin C or 50 mg of Vitamin E kg−1 produced the best antibody responses, final survival and protective response upon challenge. No conclusive inferences could be drawn on the growth responses from the experiment.
  Fish & Shellfish Immunology.