María Jesús Sagües

Comunidad de Madrid, Madrid, Madrid, Spain

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Publications (8)15.46 Total impact

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    ABSTRACT: The aim of this paper is to compare the seroprevalence of cytomegalovirus (CMV) in women in the Autonomous Region of Madrid (ARM) obtained in two different years (1993 and 1999), according to age and seroprevalence of a virus transmitted by the faecal-oral route (hepatitis A virus, HAV) and another virus of respiratory transmission (varicella zoster virus, VZV). A total of 1813 serum samples were studied, taken from females aged 2-40 in two different serosurveys which were representative of the general population in the ARM. Of these, 933 were taken in 1993, and 880 in 1999. In each survey the samples were distributed over six groups, according to age group (2-5, 6-10, 11-15, 16-20, 21-30 and 31-40 years). CMV- and VZV-specific IgG was tested by indirect ELISA (Dade-Behring, Germany); and HAV-IgG by ImX (Abbott, USA) in the 1993 samples, or by Vidas (BioMérieux, France) in the case of those taken in 1999. A significant age-related rise in CMV seroprevalence was observed in both serosurveys. The seroprevalence obtained was lower in all age groups in 1999 than in 1993. The differences were statistically significant in two age groups: 6-10 years old (43.7 vs. 56.7%) and 31-40 years old (79.1 vs. 90.3%). In the younger age groups concurrent seroprevalence of CMV and VZV was significant lower in 1999. In older age groups a significant decrease in concurrent seroprevalence of both CMV and HAV was also seen. Agreement between serological results for CMV-HAV, CMV-VZV and HAV-VVZ during the two time periods and in every age group was poor or fair (kappa index < or = 0.2 or between 0.21 and 0.4) in all age groups. To conclude, a change in CMV epidemiology seems to be taking place in Madrid. The increase in the proportion of CMV seronegative women of childbearing age may have some impact on the incidence of congenital diseases related to vertical transmission of CMV. Apparently, such a change, among children, could be related to a lower close contact transmission rate (as in VZV), and among adults to improvements in standards of public health (as in HAV). However, due to the poor or fair agreement between serological results for CMV-HAV, CMV-VZV and HAV-VVZ, other independent factors may affect the fall in CMV seroprevalence.
    European Journal of Epidemiology 01/2004; 19(1):85-9. · 5.12 Impact Factor
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    ABSTRACT: Clostridium perfringens is a classical agent of food-borne disease but because of the mildness and self-limiting nature of the illness, many cases are undiagnosed. This study describes the investigation of an outbreak of diarrhea due to C. perfringens in a public restaurant. An epidemiological survey was performed and the restaurant was inspected. The specific attack rates for the items on the menu were calculated. Odds ratios were calculated to investigate the independent association between each item and the disease using a logistic regression model. Investigation of C. perfringens toxin in the feces of four symptomatic subjects and one exposed but asymptomatic subject was performed by the reverse passive latex agglutination test. The overall attack rate was 70.8%. The main symptoms were diarrhea (100%) and abdominal pain (94%). Significant differences were found in specific attack rates for consumption of different menu items. However, the independent contribution of each item was significant only for consumption of "ravioli with cheese sauce". Fecal detection of C. perfringens enterotoxin was positive in the four symptomatic subjects and negative in the exposed but asymptomatic subject. The overall attack rate in this outbreak was high. The clinical symptomatology was similar to previously published data. The epidemiological analysis revealed "ravioli with cheese sauce" to be responsible for transmission of the disease and clinical investigation together with the fecal enterotoxin detection established C. perfringens as the etiological agent.
    Enfermedades Infecciosas y Microbiología Clínica 04/2002; 20(3):117-22. · 1.48 Impact Factor
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    ABSTRACT: Objective Clostridium perfringens is a classical agent of food-borne disease but because of the mildness and self-limiting nature of the illness, many cases are undiagnosed. This study describes the investigation of an outbreak of diarrhea due to C. perfringens in a public restaurant. Methods An epidemiological survey was performed and the restaurant was inspected. The specific attack rates for the items on the menu were calculated. Odds ratios were calculated to investigate the independent association between each item and the disease using a logistic regression model. Investigation of C. perfringens toxin in the feces of four symptomatic subjects and one exposed but asymptomatic subject was performed by the reverse passive latex agglutination test. Results The overall attack rate was 70.8%. The main symptoms were diarrhea (100%) and abdominal pain (94%). Significant differences were found in specific attack rates for consumption of different menu items. However, the independent contribution of each item was significant only for consumption of “ravioli with cheese sauce”. Fecal detection of C. perfringens enterotoxin was positive in the four symptomatic subjects and negative in the exposed but asymptomatic subject. Conclusions The overall attack rate in this outbreak was high. The clinical symptomatology was similar to previously published data. The epidemiological analysis revealed “ravioli with cheese sauce” to be responsible for transmission of the disease and clinical investigation together with the fecal enterotoxin detection established C. perfringens as the etiological agent.
    Enfermedades Infecciosas y Microbiología Clínica 01/2002; 20(3):117–122. · 1.48 Impact Factor
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    ABSTRACT: Although there are several ELISA procedures to determine IgG against Bordetella pertussis, there are scarce data that allow to compare the seroprevalence detected using different kits. The objective of this study was to evaluate and to compare by three ELISA techniques the seroprevalence against B. pertussis in children. 172 children 6-10 years old, vaccinated with three dose of DTPwc were studied. IgG against Bordetella pertussis was determined by two quantitative techniques (Labsystems and Serion). The qualitative detection of IgG against FHA and PT was performed by a semiquantitative method (Pertusscan). The Labsystems ELISA classified a 42.4% of the samples as positive and a 32% as borderline. The Serion method showed 52.9% of positive and 20.9% of borderline results. IgG-FHA was detected in 81.4% and IgG-PT in 75% of the samples by Pertusscan. The level of "immunity" recommended by this method was of 21.5%. The agreement between Labsystems and Serion was 45.9% (kappa index 5 0.157; p < 0.01). In the IgG-TP or IgG-FHA positive samples, the proportion of positive results obtained by Serion was superior to those obtained by Labsystems while the number of borderline results were inferior (p < 0.05). Both quantitative techniques showed a high rate of not conclusive results and a very weak concordance. The large number of doubtful results obtained by Labsystems and its worse concordance with the other techniques dissuades its employment in inmunogenicity studies. The results obtained by the other two techniques present a problematic interpretation in terms of seroprevalence.
    Enfermedades Infecciosas y Microbiología Clínica 01/2002; 20(1):10-5. · 1.48 Impact Factor
  • Enfermedades Infecciosas y Microbiología Clínica 04/2000; 18(3):147-8. · 1.48 Impact Factor
  • Enfermedades Infecciosas y Microbiología Clínica 21(7):383–384. · 1.48 Impact Factor
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    ABSTRACT: Background Although there are several ELISA procedures to determine IgG against Bordetella pertussis, there are scarce data that allow to compare the seroprevalence detected using different kits. The objective of this study was to evaluate and to compare by three ELISA techniques the seroprevalence against B. pertussis in children. Patients and method 172 children 6-10 years old, vaccinated with three dose of DTPwc were studied. IgG against Bordetella pertussis was determined by two quantitative techniques (Labsystems and Serion). The qualitative detection of IgG against FHA and PT was performed by a semiquantitative method (Pertusscan). Results The Labsystems ELISA classified a 42.4% of the samples as positive and a 32% as borderline. The Serion method showed 52.9% of positive and 20.9% of borderline results. IgG-FHA was detected in 81.4% and IgG-PT in 75% of the samples by Pertusscan. The level of “immunity” recommended by this method was of 21.5%. The agreement between Labsystems and Serion was 45.9% (kappa index □ 0.157; p < 0.01). In the IgG-TP or IgG-FHA positive samples, the proportion of positive results obtained by Serion was superior to those obtained by Labsystems while the number of borderline results were inferior (p < 0.05). Conclusions Both quantitative techniques showed a high rate of not conclusive results and a very weak concordance. The large number of doubtful results obtained by Labsystems and its worse concordance with the other techniques dissuades its employment in inmunogenicity studies. The results obtained by the other two techniques present a problematic interpretation in terms of seroprevalence.
    Enfermedades Infecciosas y Microbiología Clínica 20(1):10–15. · 1.48 Impact Factor
  • Enfermedades Infecciosas y Microbiología Clínica 21(7):383-4. · 1.48 Impact Factor