Lidia Beatriz Conza Blanco

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Publications (2)5.17 Total impact

  • Article: Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples.
    Chul-min Kim, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, Ikuo Igarashi
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    ABSTRACT: We developed a TaqMan real-time polymerase chain reaction (PCR) assay for the quantitative detection of Theileria equi from the in vitro-cultured parasite and field blood samples collected from horses living in Ghana and Brazil. The detection limit for the assay was determined to be 1.5 parasites/microl per sample, and the quantitative capacity was demonstrated using the in vitro-cultured parasite. For field applications, the real-time PCR assay was compared to a previously established nested PCR assay used as the gold standard for the real-time PCR assay. Of 65 field blood samples, 46 samples were T. equi-positive in the nested PCR assay, while the real-time PCR assay also detected the parasite in all 46 of the nested PCR-positive samples but did not detect T. equi in the remaining 19 negative blood samples. This quantitative real-time PCR assay provides a valuable tool for fast laboratory diagnostic assessment of T. equi infection in horses.
    Veterinary Parasitology 03/2008; 151(2-4):158-63. · 2.58 Impact Factor
  • Article: Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by Babesia bovis and Babesia bigemina.
    Chul-Min Kim, Lidia Beatriz Conza Blanco, Andy Alhassan, Hiroshi Iseki, Naoaki Yokoyama, Xuenan Xuan, Ikuo Igarashi
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    ABSTRACT: With the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (BoiICT) was constructed for the simultaneous detection of Babesia bovis- and Babesia bigemina-specific antibodies using B. bovis recombinant merozoite surface antigen-2c and B. bigemina recombinant rhoptry-associated protein-1. The BoiICT selectively detected specific antibodies to B. bovis and B. bigemina. All sera from cattle infected with other protozoan parasites (i.e., Cryptosporidium parvum, Neospora caninum, and Theileria orientalis) showed negative results in the BoiICT. The relative sensitivity and specificity for detecting antibody to B. bovis were 96.7% (29 of 30) and 91.3% (73 of 80), respectively. The relative sensitivity and specificity for detecting antibody to B. bigemina were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment.
    The American journal of tropical medicine and hygiene 02/2008; 78(1):117-21. · 2.59 Impact Factor
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