Ke-Li Luo

Publications (4)4 Total impact

• Article: [Proteomic identification of necrozoospermia-related proteins].

  Ke-Li Luo, Li-Qing Fan, Guang-Xiu Lu
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  ABSTRACT: To compare the proteomic differences between normal and necrozoospermic human spermatozoa. We isolated proteins from 30 ejaculates of 10 healthy donors and 3 specimens of a necrozoospermia patient by two-dimensional gel electrophoresis (2-DE), analyzed their 2-DE maps and identified some differentially expressed proteins by mass spectrometry. A total of (905 +/- 57) and (792 +/- 28) proteins were isolated from the spermatozoal maps of the normal and necrozoospermic men, respectively, and 178 found to be differentially expressed in that of the necrozoospermia patient. The 6 missing protein spots in the necrozoospermic map were identified as sperm protein (human, accession No. 060904), zinc finger protein 174 (AW-1), F-actin capping protein alpha-3 subunit, testis-specific inhibitor of apoptosis, death domain receptor 3 soluble form (fragment) and peptide similar to the activator of CREM in the testis. Six missing protein spots were identified in the necrozoospermic spermatozoal map which may be associated with the development of necrozoospermia.
  Zhonghua nan ke xue = National journal of andrology 06/2008; 14(5):431-5.
• Article: Establishment of a high-resolution 2-D reference map of human spermatozoal proteins from 12 fertile sperm-bank donors.

  Ling-Wei Li, Li-Qing Fan, Wen-Bing Zhu, Hong-Chuan Nien, Bo-Lan Sun, Ke-Li Luo, Ting-Ting Liao, Le Tang, Guang-Xiu Lu
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  ABSTRACT: To extend the analysis of the proteome of human spermatozoa and establish a 2-D gel electrophoresis (2-DE) reference map of human spermatozoal proteins in a pH range of 3.5-9.0. In order to reveal more protein spots, immobilized pH gradient strips (24 cm) of broad range of pH 3-10 and the narrower range of pH 6-9, as well as different overlapping narrow range pH immobilized pH gradient (IPG) strips, including 3.5-4.5, 4.0-5.0, 4.5-5.5, 5.0-6.0 and 5.5-6.7, were used. After 2-DE, several visually identical spots between the different pH range 2-D gel pairs were cut from the gels and confirmed by mass spectrometry and used as landmarks for computer analysis. The 2-D reference map with pH value from 3.5 to 9.0 was synthesized by using the ImageMaster analysis software. The overlapping spots were excluded, so that every spot was counted only once. A total of 3872 different protein spots were identified from the reference map, an approximately 3-fold increase compared to the broad range pH 3-10 IPG strip (1306 spots). The present 2-D pattern is a high resolution 2-D reference map for human fertile spermatozoal protein spots. A comprehensive knowledge of the protein composition of human spermatozoa is very meaningful in studying dysregulation of male fertility.
  Asian Journal of Andrology 06/2007; 9(3):321-9. · 1.52 Impact Factor
• Article: Newly expressed proteins of mouse embryonic fibroblasts irradiated to be inactive.

  Chang-qing Xie, Ge Lin, Ke-li Luo, Shu-wei Luo, Guang-xiu Lu
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  ABSTRACT: It has been found that post-radiation mouse embryonic fibroblasts can well maintain the pluripotency in human embryonic stem cells. However, the molecular mechanism remains unclear. In the present study, the new protein expression profile of post-radiation mouse embryonic fibroblasts was analyzed by immobilized pH gradient 2-dimensional polyacrylamide gel electrophoresis. Image analysis following silver staining revealed (969+/-57) vs. (1085+/-107) spots from post-radiation mouse embryonic fibroblasts and pre-radiation ones, respectively. Some newly expressed proteins, which were only abundantly present after irradiation, were subjected to peptide mass fingerprint analysis and identified using MALDI-TOF-MS, SWISS-PROT database, and RT-PCR. Several of those proteins were preliminarily identified to participate in cytokine secretion, cell signal transduction, transcriptional regulation, and apoptosis, etc., which suggested that inactive post-radiation mouse embryonic fibroblasts expressed some new proteins that may underlie the molecular mechanisms to maintain the pluripotency in human embryonic stem cells.
  Biochemical and Biophysical Research Communications 04/2004; 315(3):581-8. · 2.48 Impact Factor
• Article: [Diagnosing achondroplasia by single cell nested-PCR].

  Chang-gao Zhong, Lu-yun Li, Chang-fu Lu, Ge Lin, Jun-jiang Fu, Ke-li Luo, Guang-xiu Lu
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  ABSTRACT: To research on the reliability of diagnosing achondroplasia (ACH) on single cell level and to provide a basis for preimplantation genetic diagnosis(PGD). The high-frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested-PCR with single lymphocyte and single blastomere. The products of PCR were digested by restriction enzyme Bfm I, then the digested products were detected by 10% polyacrylamida gel electrophoresis(PAGE). The amplification success rate, allele dropout rate and correct diagnosis rate of single lymphocyte's PCR were 90.4%, 8.2% and 91.8%,respectively. The amplification success rate of single blastomere was 75.4%. The diagnosis of ACH by single cell nested-PCR is comparatively stable and reliable.
  Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 07/2003; 20(3):228-31.