Karim M.R

Publications (5)0 Total impact

• Article: Comparative Cytotoxicity Study of Six Bioactive Lectins Purified from Pondweed ( Potamogeton nodosus Poir) Rootstock on Brine Shrimp

  Pervin F, Hossain M.M, Khatun S, Siddique S.P, Salam K.A, Karim M.R, Absar N
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  ABSTRACT: Six bioactive lectins were purified from the rootstock of Pondweeds ( Potamogeton nodosus Poir) by conventional chromatographic methods. They showed cytotoxic effect in brine shrimp ( Artemia salina L.) lethality bioassay. The LD-50values of PNL-1, PNL-2, PNL-3, PNL-4, PNL-5 and PNL-6 were found to be 10.76, 7.03, 17.25, 10.52, 19.60 and 20.1 μg mL^-1, respectively which implied that they have significant uses specially of PNL-2. Experimental results revealed that PNL-2 and PNL-4 were more cytotoxic than other lectins.
  Journal of Medical Sciences. 01/2006;
• Article: Field Evaluation of Integrated Management Practices on Garlic Production

  Karim M.R, Asaduzzaman S.M, Islam F, Islam M.A, Islam S.M.N, Naher Q
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  ABSTRACT: Two types of fungicides and their mixture viz. Rovral, Ridomil and Rovral+Ridomil along with five combinations of different fertilizers were tested to find the best integrated package method for successful garlic production. The highest fertilizer dose especially nitrogenous fertilizer F₅ (200, 166, 200, 20 and 1000 kg N, P₂O₅, K₂O, S and Cow dung ha ^-1) produced the highest bulb yield of garlic (6.36 and 7.89 t ha ^-1 during 1997 and 1998 respectively), which was statistically similar with the yield of F₄ (6.28 and 7.57 t ha 1 during 1997 and 1998 respectively) where 150, 166, 200, 20 and 1000 kg N, P₂O₅, K₂O, S and CD ha ^-1 were applied. The mixed application of Rovral and Ridomil was better in case of garlic bulb yield (6.44 and 7.79 t ha 1 in 1997 and 1998 respectively). The incidence of purple blotch was significantly reduced (1.6 and 1.7 score in 1997 and 1998 respectively) by mixed application fungicide. The interaction between fertilizer and fungicide had no significant effect on garlic yield.
  Pakistan Journal of Biological Sciences. 01/2002;
• Article: Purification and Characterization of Polyphenoloxidase from Guava Infected with Fruit-rot Disease

  Razzaque M.A, Saud Z.A, Absar N, Karim M.R, Hashinaga F
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  ABSTRACT: Polyphenoloxidase (PPO), an important oxidative enzyme which played a vital role in the brewing of guava after infection with fruit-rot disease was purified from disease-affected guava. The method was accomplished by ion exchange chromatography of crude enzyme extract on DEAE-cellulose followed by CM-cellulose. The purified enzyme was found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 178 kDa and 180.5 kDa by gel filtration and SDS-PAGE respectively. The enzyme appeared to be a single polypeptide chain as revealed by SDS-PAGE either in presence or in absence of reducing agent. Optimum activity of the enzyme was at 48?C and pH around 8. Activity was destroyed completely in presence of EDTA, a metal chellator. Purified PPO showed an estimated k<SUB>m</SUB> value of 4.4 mM for catechol as substrate.
  Pakistan Journal of Biological Sciences. 01/2000;
• Article: A Comparative Analysis on the Purification and Characterization of Amylase from the Flesh of Healthy and Disease-affected Moringa Fruit ( Moringa oleifera Lam)

  Rahman M.Z, Saud Z.A, Absar N, Karim M.R, Hashinaga F
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  ABSTRACT: Two different amylase from the flesh of healthy and fruit-rot disease-affected Moringa fruit were purified by successive chromatography of the crude enzyme extract on DEAE-cellulose followed by CM-cellulose and were purified 46- and 50-fold respectively. Both the enzymes appeared to be homogeneous as judged by polyacrylamide gel electrophoresis. Molecular weights of amylases from healthy and diseased Moringa estimated by gel filtration were 59 kDa and 66.5 kDa respectively. The purified enzymes were classified as -amylase and consist of a single polypeptide chain. The amylase from healthy and diseased Moringa flesh showed the following characteristics: pH optima, 6.8 and 6.4; temperature optima, 38 ?C and 40 ?C; K<SUB>m</SUB> value, 0.28 and 0.22% for starch as substrate respectively.
  Pakistan Journal of Biological Sciences. 01/2000;
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  Article: Purification and Partial Characterization of a Protease from Tomato ( Lycopersicon esculentum Mill)

  Karim M.R, Islam M.A, Absar N, Hashinaga F
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  ABSTRACT: A protease from tomato flesh was purified to homogeneity by (NH₄)₂SO₄ precipitation followed by Sephadex G-75 and DEAE-cellulose column chromatography. Molecular weight of the enzyme was estimated to be 81-79 KD by gel filtration and SDS-PAGE respectively. The enzyme was found to be a single polypeptide chain as revealed by SDS-PAGE under either reducing or non-reducing conditions. Optimum activity was observed at pH 7.0 and 45�C with a k_m value of 0.48 per cent determined by using casein as substrate. The enzyme appears to be a serine protease being inhibited greatly by DFP and PMSF and to a lesser extent by heavy metals such as Pb²⁺ and Fe²⁺.
  Pakistan Journal of Biological Sciences. 01/1999;