Ingrid Brucknerova

Slovak Academy of Sciences, Bratislava, Bratislavsky Kraj, Slovakia

Are you Ingrid Brucknerova?

Claim your profile

Publications (27)20.1 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the study was to determine the value of the total antioxidant system (TAS) and level of malondialdehyde (MDA), and the activity of glutathione peroxidase, superoxide dismutase in the case of premature twins - identical twins, monozygotic, on the 1st and 5th day of life and to compare the values between the first-born twin A and the second-born twin B. We confirmed the difference between A and B twins in values of TAS and MDA, as well as the difference between the 1st and 5th day of life. The values of TAS, which show the total activity of antioxidant enzymes in a newborn's organism, reflect the ability of protection against oxidative stress before and during delivery. In the case of twin pregnancy, the value of TAS is crucial and determines the degree and severity of consequences of asphyxia. MDA values indicate the presence of lipoperoxidation.
    Neuro endocrinology letters 11/2013; 34(Suppl2):71-73. · 0.93 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Approximately 3% of annual births suffer from birth asphyxia and one million of these newborns die. The aim of this study was to develop a model for studying subchronic perinatal asphyxia (SPA) in rats. Pregnant animals were exposed to 10.5% O2 during sensitive stages of brain development for 4 hours a day. Biochemical variables were analyzed immediately and 24 hours after asphyxia. SPA caused significant reduction of foetal weight, produced abnormalities of distal parts of the skeleton, and anomalies in the development of brain ventricles. Time-dependent changes were observed in several parameters indicating adjustment of the developing organism to the delivery. Whereas lactate was elevated immediately after asphyxia, glucose mirrored high energy needs 24 hours after the insult. Immunohistochemical examination of the placentas revealed overgrowth of acidic glycoconjugates in the extracellular matrix of vascular walls in the animals exposed to asphyxia. We observed the presence of muscle fibres in chorionic plate arteries and also in intraplacental arteries. The present model proved to be useful for the study of asphyxial conditions during pregnancy. As it is non-invasive and allows to control asphyxial conditions, it appears suitable for the screening and investigation of indicators of asphyxia in the mother and foetus.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 07/2013; · 2.99 Impact Factor
  • Ingrid Brucknerova, Anna Holomanova
    [Show abstract] [Hide abstract]
    ABSTRACT: The paper highlights the personality of the founder of modern anatomy, who was able to use his knowledge and skills to change the view on the construction of the human body extending over centuries. He introduced a new scientific approach and highlighted the importance of autopsies for understanding of human body which carefully demonstrated and documented. De humani corporis fabrica - the spectacular work, in which he summarized results of his theoretical and practical findings, has opened a new path for the study of anatomy. Andreas Vesalius became a pioneer in the history of medical education. In 2014 will pass 500 years since his birth.
    Neuro endocrinology letters 01/2013; 34(6):498-500. · 0.93 Impact Factor
  • Ingrid Brucknerova, Anna Holomanova, Mojmir Mach, Eduard Ujhazy
    [Show abstract] [Hide abstract]
    ABSTRACT: The paper highlights the personality of the founder of European student exchange program ERASMUS (EuRopean Community Action Scheme for the Mobility of University Students) Erasmus of Rotterdam. He was one of the leading European humanists and has left a literary legacy of large dimensions. His thoughts, ideas, opinions, and mainly the works have a great benefit for society even today. From 16th century to the present time they are the subject of unchanged interest.
    Neuro endocrinology letters 01/2013; 34(6):501-3. · 0.93 Impact Factor
  • Ingrid Brucknerova, Anna Holomanova, Eduard Ujhazy, Mojmir Mach
    [Show abstract] [Hide abstract]
    ABSTRACT: It is well known that the names of discoverers are commonly used in medical terminology, especially in everyday use. The advantage of an eponym is that a complex description of a certain syndrome, surgical procedure, manifestations of a particular disease, or its details, can be expressed in a single word (Holomáňová & Brucknerová 2003). Current official anatomical nomenclatures do not use eponyms. Some exceptions do however exist. In pediatrics, we frequently use the names of two famous persons: Virginia Apgar and Ernst Moro.
    Neuro endocrinology letters 12/2012; 33(8). · 0.93 Impact Factor
  • Ingrid Brucknerova, Anna Holomanova, Mojmir Mach, Eduard Ujhazy
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study is to present a short biography of some important physicians and describe the most prominent differences between trisomy 13, 18 and 21. The authors present the most prominent differences between trisomy 13, 18 and 21. The work of many important physicians, geneticists, has helped in the process of recognition of congenital anomalies. This group of famous persons includes Patau, Edwards and Down.
    Neuro endocrinology letters 12/2012; 33(7). · 0.93 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Teratology is the science that studies the causes, mechanisms, and patterns of abnormal development. The authors present an updated overview of the most important milestones and stages of the development of modern teratology. Development of knowledge and society led to the recognition that causes of congenital developmental disorders (CDDs) might be caused by various mechanical effects, foetal diseases, and retarded or arrested development of the embryo and foetus. Based on the analysis of the historical development of hypotheses and theories representing a decisive contribution to this field, we present a survey of the six Wilson's fundamental principles of teratology. The aim of observing these principles is to get insight into developmental relations and to understand mechanisms of action on the level of cell populations (elementary morphogenetic processes), tissues and organs. It is important to realise that any negative intervention into the normal course of these processes, either on genetic or non-genetic basis, inevitably leads to a sequence of subsequent changes resulting in CDDs. Moreover, the classical toxicologic monotonic dose-response paradigm recently has been challenged by the so-called "low dose-hypothesis", particularly in the case of endocrine active substances. These include some pesticides, dioxins, polychlorobiphenyls (PCBs), and bisphenol A. Despite modern approaches of molecular biology and genetics, along with top diagnostic techniques, we are still not able to identify the actual cause in more than 65 to 70% of all congenital defects classified as having an unknown etiology. Today CDDs include any birth defect, either morphological, biochemical, or behavioural.
    Interdisciplinary toxicology 12/2012; 5(4):163-8.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The present study deals with effect of prenatal and neonatal administration of the synthetic pyridoindole derivative SMe1EC2 (2-ethoxycarbonyl-8-methoxy-2,3,4,4a,5,9b-hexahydro-1H-pyrido-[4,3b] indolinium chloride) on postnatal and neurobehavioral development of the rat offspring. The substance tested was administered to pregnant rats orally in the doses 5, 50 and 250 mg/kg from day 15 of gestation to day 10 post partum (PP). From the day 4 PP, the postnatal development and neurobehavioral characteritics of offspring were evaluated. The following variables were observed: body weight, pinna detachment, incisor eruption, ear opening, eye opening, testes descent and vaginal opening, righting reflex, negative geotaxia, startle reflex, dynamic air righting and exploratory behavior in a new environment. No maternal death, abortion or dead fetuses occurred either in the control or SMe1EC2 groups. Dynamic righting reflex was delayed one day in the groups of animals treated via their mothers with 5 and 50 mg/kg SMe1EC2. The delay in the development of this reflex was only transient. On day 20 PP, all pups tested had a positive score of the reflex. Administration of SMe1EC2 did not reveal any significant changes in other variables of somatic growth and maturation, reflex and neuromotor development and exploratory behavior, either of young or adult animals of both genders, assessed by analysis of variance.
    Interdisciplinary toxicology 03/2011; 4(1):47-51.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Asphyxia of the newborn has a varied etiology. Clinical consequences have a broad spectrum of presentations. Arteriovenous malformation associated with an aneurysm of the Galen vein can be the cause of focal ischemic changes in the nervous parenchyma. The authors report a case of a term newborn (birth weight 4, 000 grams, Apgar score 7/9). Physical investigation confirmed the presence of a continuous murmur in the area of the anterior fontanelle. Ultrasonic investigation of the brain detected a huge arteriovenous malformation of the Galen vein. Ultrasonic investigation of the heart excluded structural anomaly, but confirmed a huge retrograde flow in the aorta descendens, opened ductus arterious with suspected formation of coarctation of the aorta and dilatation of the vena cava superior. Congenital anomaly of the Galen vein has a negative influence on prenatal and postnatal development of the brain of a newborn. In the case of our patient, it led to rapid severe asphyxiated changes of the brain parenchyma. Diagnosis and management were established, yet endovascular therapy was not indicated in the early neonatal period.
    Neuro endocrinology letters 01/2011; 32 Suppl 1:5-7. · 0.93 Impact Factor
  • Fuel and Energy Abstracts 01/2011; 205.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To present the case of a term newborn with rapid progression of signs of neurodegenerative disease. In a case of a term newborn with numerous dysmorphic features, with seizure activity from the 3rd day of life, hypertonia and serious changes on brain parenchyma were presented. Diagnosis of molybdenum cofactor deficiency was confirmed by the decreased level of uric acid, 31 μmol/l, in serum, increased excretion of thiosulfate and S-sulfocysteine in urine, taurine (1729.3 μmol/mmol crea; normal range 30-300 μmol/mmol crea) and xanthine (276.9 μmol/mmol crea; normal range < 25 μmol/mmol crea) in urine. Sulfite oxidase activity on skin fibroblasts in culture was not detectable. The patient died at the age of 28 days of life. Deficiency of molybdenum cofactor leads to accumulation of toxic metabolites (levels of sulfite), which causes disturbances of neurotransmitters even before delivery. Therapy is symptomatic, no effective therapy is available. Seizures are difficult to suppress. This case report is about the first patient in Slovakia.
    Neuro endocrinology letters 12/2010; 31 Suppl 2:5-7. · 0.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To present clinical and laboratory findings in the case of a term newborn with conjugated hyperbilirubinaemia and to stress the importance of differential diagnosis. A term newborn delivered by caesarean section (birth weight 2550 g, birth length 47 cm, value of Apgar score 9/10) with good direct adaptation had on the first day of life increased levels of conjugated bilirubin (23 micromol/l), unconjugated bilirubin (55 micromol/l) and C-reactive protein 39.4 g/l. The diagnosis of mevalonic aciduria was confirmed by urine analysis (mevalonolactone 393 micromol/mmol crea, normal range <2.0 micromol/mmol crea; mevalonic acid 40.5 micromol/mmol crea, normal range <0.04 micromol/mmol crea). Mevalonic aciduria can be clinically distinguished based on symptoms of neurological involvement. It can also present itself with hepatosplenomegaly, lymphadenopathy, anaemia, leukocytosis, increased sedimentation rates and levels of C-reactive protein. In cases of conjugated hyperbilirubinaemia of unknown aetiology it is important to exclude mevalonic aciduria by urine investigation for organic acids.
    Neuro endocrinology letters 12/2009; 30 Suppl 1:29-31. · 0.93 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The important role of equilibrium of environmental factors during the embryo-fetal period is undisputable. Women of reproductive age are increasingly exposed to various environmental risk factors such as hypoxia, prenatal viral infections, use of drugs, smoking, complications of birth or stressful life events. These early hazards represent an important risk for structural and/or functional maldevelopment of the fetus and neonates. Impairment of oxygen/energy supply during the pre- and perinatal period may affect neuronal functions and induce cell death. Thus when death of the newborn is not occurring following intrauterine hypoxia, various neurological deficits, including hyperactivity, learning disabilities, mental retardation, epilepsy, cerebral palsy, dystonia etc., may develop both in humans and in experimental animals. In our animal studies we used several approaches for modeling hypoxia in rats during pregnancy and shortly after delivery, i.e. chronic intrauterine hypoxia induced by the antiepileptic drug phenytoin, neonatal anoxia by decreased oxygen saturation in 2-day-old pups. Using these models we were able to test potential protective properties of natural (vitamin E, melatonin) and synthetic (stobadine) compounds. Based on our results, stobadine was also able to reduce hypoxia-induced hyperactivity and the antioxidant capacity of stobadine exceeded that of vitamin E and melatonin, and contrary to vitamin E, stobadine had no adverse effects on developing fetus and offspring.
    Interdisciplinary toxicology 03/2009; 2(1):28-32.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Exposure of the developing organism to industrial chemicals and physical factors represents a serious risk factor for the development of neurobehavioral disorders, such as attention-deficit hyperactivity disorder, autism and mental retardation. Appropriate animal models are needed to test potentially harmful effects and mechanisms of developmental neurotoxicity of various chemical substances. However, there are significant human vs. rat differences in the brain developmental profile which should be taken into account in neurotoxicity studies. Subtle behavioral alterations are hard to detect by traditional developmental toxicity and teratogenicity studies, and in many cases they remain hidden. They can however be revealed by using special behavioral, endocrine and/or pharmacological challenges, such as repeated behavioral testing, exposure to single stressful stimulus or drugs. Further, current neurobehavioral test protocols recommend to test animals up to their adulthood. However some behavioral alterations, such as anxiety-like behavior or mental deficiency, may become manifest in later periods of development. Our experimental and scientific experiences are highly suggestive for a complex approach in testing potential developmental neurotoxicity. Strong emphasis should be given on repeated behavioral testing of animals up to senescence and on using proper pharmacological and/or stressful challenges.
    Interdisciplinary toxicology 12/2008; 1(3-4):206-10.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Acute perinatal asphyxia is a major cause of death and neurological injury in newborn infants. Severe asphyxia can occur in infants around the time of birth for several reasons. The aim of our study was to find the most sensitive, easily obtainable and fast assessable parameter of the presence and quantification of asphyxia.In our study 39 term newborns (15 healthy term newborns and 24 asphyxial term newborns), from vaginal deliveries admitted within 24 hours of life were monitored and parameters of blood count from venous blood were assessed. Laboratory findings of blood count parameters revealed significant differences between term asphyxial and healthy newborns in erythrocyte count and hemoglobin and hematocrit values.Hematological changes observed early after delivery can determine the duration of hypoxemia (acute vs. chronic) and asphyxia of short duration may be accompanied without occurrence of polyglobulia.
    Interdisciplinary toxicology 12/2008; 1(3-4):211-3.
  • [Show abstract] [Hide abstract]
    ABSTRACT: To present a term newborn with severe asphyxial status due to dysrrhythmia induced by the neonatal form of carnitine palmitoyltransferase II deficiency (CPT II). Term newborn delivered spontaneously (birth weight 3450 grams, birth length 52 cm, values of Apgar score 10/10) with good direct adaptation, on second day of life he manifested severe asphyxial status followed by cardiorespiratory insufficiency with circulatory failure. After prolonged resuscitation of 3 hours, the child was admitted to our neonatological department. Diagnosis of CPT II was confirmed (free carnitine level in blood 12.2 micromol/l; ratio (C16+C18):1/C2 was 0.760 by tandem mass spectrometry; activity of CPT II in leukocytes was 0.082 micromol/min x gram protein). After appropriate treatment the patient survived the critical period. Neonatal form of CPT II deficiency is the most severe form and is considered to be invariably fatal. This kind of metabolic disease is congenital, but cardiac problems are not detectable during the prenatal period. Fasting in the early newborn period is a main trigger of CPT II deficiency signs. The authors emphasise the relevance of investigating acylcarnitine profiles and carnitine in serum in all cases of severe postnatal asphyxia and in cases of unusual newborn arrhythmias since some forms of disturbances in beta oxidation of fatty acids are partially treatable.
    Neuro endocrinology letters 11/2008; 29(5):627-30. · 0.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The 2-ethoxycarbonyl-8-methoxy-2,3,4,4a,5,9b-hexahydro-1H-pyrido-[4,3b] indolinium chloride (SMe1EC2) is a prospective antioxidant and neuroprotectant drug. The aim of the study was to evaluate the effect of SMe1EC2 on embryofetal development of rats. The substance tested was administered orally to Wistar/DV rats from day 6 to day 15 of gestation at the doses 5, 50 and 250 mg/kg/day. The animals were killed on day 20 of gestation and uterine content was inspected. Live fetuses were examined for gross, skeletal and visceral anomalies. Administration of SMe1EC2 did not induce any signs of maternal toxicity. No adverse effect of the substance tested was found on reproductive variables. Morphological examination of fetuses revealed no evidence of teratogenesis. The prenatal toxicity study showed that the substance SMe1EC2 tested did not have embryotoxic and teratogenic effects on developing rats. Neither were any signs of maternal toxicity found.
    Neuro endocrinology letters 11/2008; 29(5):639-43. · 0.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Intrauterine growth retardation (IUGR) is associated with fetal adverse conditions. The most important cause of growth restriction and poor perinatal outcome is chronic fetal hypoxemia (CFH). Adaptation to CFH can be studied by Doppler velocity waveform on umbilical and fetal arteries and cardiotocography (CTG). Preterm delivery, as an elimination of CFH, has to be confronted with the risks of prematurity. A special situation may occur when CTG is normal at the absence of end-diastolic velocity (AEDV). AEDV in the umbilical artery precedes the onset of abnormal CTG, whose duration differs considerably among the fetuses. The time after the onset of AEDV in pregnancy may be utilized for performing exact diagnosis by fetal blood analysis. Primigravida at 30 gestational weeks was referred because of IUGR. IUGR, AEDV, oligohydramnion, and normal fetal anatomy were revealed. CTG was normal. Indication for cordocentesis was to perform cord blood gases analysis and to obtain fetal caryotype. Cordocentesis revealed normal caryotype, values of pH, and fetal blood gases were considered satisfactory. Continuation of pregnancy was decided in spite of persistent AEDV. At 33 gestational weeks pathological CTG was an indication for induction of labor. Labor, delivery, umbilical blood gases, postpartal and neonatal outcome were normal. In the case of fetal monitoring controversy assessment of umbilical blood analysis may be crucial. This examination is significant and independent of the interval between cordocentesis and the onset of CTG pathology. This interval may be utilized for intrauterine treatment and for optimizing obstetric management.
    Neuro endocrinology letters 11/2008; 29(5):635-8. · 0.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: In healthy term newborns (HTN) to determine on the 1st and 5th day of life the activity of total antioxidant capacity (TAS), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPX) and to compare the values with the group of asphyxiated term newborns (ATN). The series consisted of 15 HTN and 24 ATN. In both groups TAS, MDA, GPX and SOD were investigated. Reference values in HTN (1st/5th day of life) for TAS were 0.52+/-0.03/0.49+/-0.04 mmol/l, for MDA 0.72+/-0.07/1.08+/-0.09 micromol/l, for SOD 594.20+/-16.47/591.23+/-14.14 Ug/Hb and for GPX 25.48+/-1.32/25.98+/-1.20 Ug/Hb. In a group of ATN the obtained values were (1st/5th day of life): TAS 1.1+/-0.08/0.98+/-0.08 mmol/l, MDA 2.08+/-0.22/2.21+/-0.34 micromol/l, SOD 509.18+/-26.8/564.49+/-36.4 Ug/Hb and GPX 30.2+/-1.9/32.45+/-2.69 Ug/Hb. Statistically significant differences were found on the 1st and 5th day of life between the two groups investigated in values of MDA (**p<0.01) and TAS (**p<0.01). Increased values of MDA in the group of ATN on the 1st and 5th day of life confirmed the presence of lipoperoxidation. The obtained values of TAS on the 1st and 5th day of life in the group of ATN were surprisingly higher than in HTN. The increase of TAS in ATN could point to a certain ability of ATN to prevent the damage of balance between overproduction of MDA and antioxidants. The results of SOD and GPX activity were not statistically significant, yet they are indicative of the biochemical reaction of the organism of term newborns to asphyxia.
    Neuro endocrinology letters 01/2007; 27 Suppl 2:65-8. · 0.93 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to evaluate the effect of the synthetic pyridoindole antioxidant stobadine (STO, 50mg/kg/day) on behavioural alterations induced by acute neonatal anoxia in rat offspring. STO was administered orally from day 15 of gestation up to day 7 post partum (PP). Pups of both genders were exposed to anoxia (100% N(2) for 10 minutes in a special glass chamber) on day 6 PP. After the anoxic insult, neurobehavioural development of rats (somatic growth and maturation, neuromotor and reflex development, spontaneous behaviour in open field) was evaluated up to adulthood. Neonatal anoxia did not affect somatic growth and maturation. The anoxic insult resulted in hyperactivity of male offspring in the open field test. STO pretreatment was found to decrease the anoxia induced hyperactivity in male offspring, and affected also the performance of pups on rotating rod. Since STO reduced anoxia-induced hyperactivity, we suggest its potential protective effect in acute hypoxic insults in the perinatal period.
    Neuro endocrinology letters 01/2007; 27 Suppl 2:82-5. · 0.93 Impact Factor