Ingrid Brucknerová

Comenius University in Bratislava, Presburg, Bratislavský, Slovakia

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Publications (36)25.86 Total impact

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    ABSTRACT: Folate plays one of the most important functions for nucleotide biosynthesis and cellular methylation reactions in cells. Folate-mediated one-carbon metabolism is essential for metabolic processes in the human body. During periods of rapid cell growth, such as perinatal period, increased amounts of folate are required. The determination of red blood cell (RBC) folate concentration levels is the most accurate indicator of long-term folate level status in the body. This prospective study determined RBC folate concentration levels on the first day of life from umbilical cord blood samples in the whole group of full-term newborns (n = 150), who were hospitalized at the Department of Neonatology at the University Hospital in Bratislava. Immunochemical analysis for the determination of folate levels in erythrocytes was performed (Roche Diagnostics, Germany). Mothers were asked to select different types of food and use folic acid or other multivitamin supplements containing also folic acid. Our results of RBC folate ranged from 625 to 1748 ng/mL (5th-95th percentile). The median was 935 ng/mL and deficiency was not observed in any sample. RBC folate concentration levels in newborns due to mother's intake of multivitamin supplements were significantly increased (p = 0.02). No differences were observed in the levels of RBC folate concentration when mothers used only folic acid. The RBC folate concentration tended to change based on many factors on both the mother's and the newborn's sides. Our results showed different results of RBC folate when focused on neonatal period and maternal intake of vitamins during pregnancy.
    Biologia 12/2015; 69(12):1784-1789. DOI:10.2478/s11756-014-0482-5 · 0.70 Impact Factor
  • T Revakova · A Vasilenkova · E Ujhazy · M Dubovicky · M Mach · I Brucknerova
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    ABSTRACT: To determine whether red blood cell (RBC) folate concentration levels are correlated with the occurrence of neonatal asphyxia and to study the effects of gestational age, gender, and mode of delivery on RBC folate concentration levels in newborns. Asphyxia is one of the frequent causes of morbidity and mortality of newborns. Severe perinatal asphyxia can arise due to many factors. In a prospective study, the RBC folate concentrations were determined on day 1 of life in the whole group (n = 181) of full-term (n = 121) and preterm (n = 60) newborns. Immunochemical analysis for the determination of folate in erythrocytes was performed. RBC folate concentration levels in asphyxiated newborns (n = 16) were significantly decreased (median 974 ng/ml; p = 0.023) in comparison with healthy newborns. On the other hand, the RBC folate concentration levels were significantly increased in preterm newborns (median 1,212 ng/ml; p = 0.01) in comparison with full-term newborns (median 1,098 ng/ml). Higher RBC folate concentration levels were found in newborns which had been delivered by Caesarean section (median 1,188 ng/ml; p = 0.02) compared to those born vaginally (median 1,098 ng/ml). Our results confirmed a significant decrease in RBC folate concentration in asphyxiated newborns on their first day of life (Fig. 4, Ref. 36).
    Bratislavske lekarske listy 01/2015; 116(7):417-21. · 0.45 Impact Factor
  • T Revakova · O Revak · A Vasilenkova · D Behulova · I Brucknerova
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    ABSTRACT: Folic acid is one of the important supplements for adequate development during pregnancy. A higher intake of folic acid during pregnancy and lactation is recommended. In prospective study the group of premature newborns (n = 52) and the type of nutrition were observed. The red blood cell (RBC) folate concentration levels were determined in the first 24 hours after delivery, before and after oral treatment with folic acid (100 µg/day). Immunochemical analysis for the determination of folate in erythrocytes was performed. In the group of premature newborns the RBC folate concentration levels after treatment were significantly increased (p < 0.0001). A dynamic increase in the concentration levels of folates in erythrocytes before and after treatment was observed, in spite of the reciprocally decreasing blood count parameters, but without any statistically significant correlation. The infant nutrition before treatment contained significantly higher amount of folic acid in fortified breast milk compared to breast milk (p = 0.04). The various types of nutrition for preterm newborns contain a different amount of folic acid. It poses a question of real needs of these patients. The best way to determine the optimal whole amount of folate is to know the real levels of folate in premature newborns (Tab. 2, Fig. 5, Ref. 26).
    Bratislavske lekarske listy 01/2015; 116(6):349-53. DOI:10.4149/BLL_2015_066 · 0.45 Impact Factor
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    ABSTRACT: Perinatal asphyxia is one of the major cause of mortality in newborns and cause of neurological disorders in adulthood. Brain damage is of the most concern due to high sensitivity of nervous system to suboptimal intrauterine oxygen condition. The aim of this study was to assess effect of subchronic prenatal asphyxia (SPA) during sensitive stages of brain maturation on behavioral changes in rats, as a method of prenatal programming of anxiety and depression-like behavior. Pregnant Wistar/DV females were exposed to environment containing lower oxygen (10.5% O2) during sensitive stages of brain maturation (day 19-20 of gestation) for 4h a day and anxiety- and depression-like behaviors in offspring were assessed using battery of behavioral tests - Open field (OF), Elevated plus maze (EPM), Light/dark test (L/D), Forced swim test (FST), and Stress induced hyperthermia (SIH). OF did not induced changes of locomotor and exploration activities. The anxiety-like behavior was induced by SPA in EPM and L/D. These results were significant in males SPA group only. The higher response to the stress stimulus in SIH was recorded in both males and females SPA group. The intensity of climbing on the walls of cylinder in FST in males SPA group was significantly decreased indicating depression-like behavior in adulthood. In conclusion, we found out that perinatal asphyxia on 19th and 20th day of gestation caused anxiety- and depression-like behaviors in the rat offsprings. Our model of SPA has proved to be useful to study the conditions of asphyxia during pregnancy, and could be suitable model for studies uncovering the mechanisms of prenatal programing of psychiatric diseases.
    Neuro endocrinology letters 11/2014; 35(Suppl 2):214-217. · 0.94 Impact Factor
  • Ingrid Brucknerová · Mojmir Mach · Michal Dubovický · Eduard Ujházy
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    ABSTRACT: Neuroglial heterotopia is a rare developmental abnormality. Most frequently the diagnosis is established at birth or in early childhood by a typical clinical presentation. Neuroglial heterotopia can be intracranial or extracranial. A typical example of extracranial heterotopia is nasal glioma, which can be isolated or can communicate directly with the intracranium. The most sensitive investigation for the confirmation of its site is magnetic resonance imaging. Histological investigation is crucial in establishing the diagnosis. The authors present the case of postnatally assessed nasal glioma. They emphasize the importance of detailed prenatal investigation as most important in preventing birth trauma and consequent complications.
    Neuro endocrinology letters 11/2014; 35(Suppl 2):211-213. · 0.94 Impact Factor
  • Ingrid Brucknerová · Eduard Ujházy
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    ABSTRACT: The aim of this review is to present the summarisation of the knowledge about biochemical and clinical changes that occur under the influence of asphyxia as well as about the treatment. Results of experimental works in animal models bring us the explanation about many postasphyxiated changes and help us to understand the pathophysiological changes and consequences of asphyxia. The authors present the most prominent consequences of neonatal asphyxia in clinical and experimental conditions. Asphyxia significantly contributes to neonatal morbidity and mortality and determines the prognosis of future development. New insights into the pathophysiology of birth asphyxia provide the opportunity how to prevent permanent damage by the activation of the fundamental molecular processes. Care of newborn asphyxia requires teamwork. Prognosis of the asphyxiated newborn is still dependent on developmental stage (gestational age), the length and intensity of asphyxia, on the level of medical care.
    Neuro endocrinology letters 11/2014; 35(Suppl 2):201-210. · 0.94 Impact Factor
  • T Zikavska · I Brucknerova
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    ABSTRACT: Objectives: Extremely high concentration of folates in premature newborns: case reports.Background: Folates are a group of water soluble compounds, which are important for metabolic processes in human body. These are important during periods of rapid cell growth. The most accurate indicator of long-term folate level status in the body is the determination of red blood cell (RBC) folate concentrations. The optimal level of RBC folate is not known in neonatal period. Authors discuss the reasons for extremely high level of RBC folate concentrations. Methods: In our work we present the cases of two premature newborns with extremely high level of RBC folate concentrations, which were analyzed immunochemically on the first day of life and after six weeks of life.Results: In both cases we measured RBC folate concentrations on the 1st day of life. After 6 weeks we found extremely high RBC folate concentration level (5516.67 ng/ml) in the first case after RBC transfusions. In second case after two months of life the RBC folate concentration level was doubled (2335.1 ng/ml) until 24 hours after RBC transfusion compared to levels after birth.Conclusion: The normal range of RBC folate values vary in newborns. The upper limit of daily dose of folic acid in pregnancy and neonatal period is not known. On the other hand it is an easily excreted water-soluble vitamin but in premature newborn it can lead to the disruption of metabolic balance and slow its degradation. Some factors can have an impact on RBC folate concentration. Blood transfusion can be one of the main influences on RBC folate concentration. To clarify these mechanisms further studies are required (Ref. 29). Keywords: newborn, folic acid, anaemia, red blood cell folate concentrations, blood transfusion.
    Bratislavske lekarske listy 01/2014; 115(2):103-6. DOI:10.4149/BLL_2014_022 · 0.45 Impact Factor
  • Ingrid Brucknerova · Eduard Ujhazy · Michal Dubovicky · Mojmir Mach
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    ABSTRACT: The aim of the study was to determine the value of the total antioxidant system (TAS) and level of malondialdehyde (MDA), and the activity of glutathione peroxidase, superoxide dismutase in the case of premature twins - identical twins, monozygotic, on the 1st and 5th day of life and to compare the values between the first-born twin A and the second-born twin B. We confirmed the difference between A and B twins in values of TAS and MDA, as well as the difference between the 1st and 5th day of life. The values of TAS, which show the total activity of antioxidant enzymes in a newborn's organism, reflect the ability of protection against oxidative stress before and during delivery. In the case of twin pregnancy, the value of TAS is crucial and determines the degree and severity of consequences of asphyxia. MDA values indicate the presence of lipoperoxidation.
    Neuro endocrinology letters 11/2013; 34(Suppl2):71-73. · 0.94 Impact Factor
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    ABSTRACT: Approximately 3% of annual births suffer from birth asphyxia and one million of these newborns die. The aim of this study was to develop a model for studying subchronic perinatal asphyxia (SPA) in rats. Pregnant animals were exposed to 10.5% O2 during sensitive stages of brain development for 4 hours a day. Biochemical variables were analyzed immediately and 24 hours after asphyxia. SPA caused significant reduction of foetal weight, produced abnormalities of distal parts of the skeleton, and anomalies in the development of brain ventricles. Time-dependent changes were observed in several parameters indicating adjustment of the developing organism to the delivery. Whereas lactate was elevated immediately after asphyxia, glucose mirrored high energy needs 24 hours after the insult. Immunohistochemical examination of the placentas revealed overgrowth of acidic glycoconjugates in the extracellular matrix of vascular walls in the animals exposed to asphyxia. We observed the presence of muscle fibres in chorionic plate arteries and also in intraplacental arteries. The present model proved to be useful for the study of asphyxial conditions during pregnancy. As it is non-invasive and allows to control asphyxial conditions, it appears suitable for the screening and investigation of indicators of asphyxia in the mother and foetus.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 07/2013; DOI:10.1016/j.fct.2013.07.023 · 2.61 Impact Factor
  • Ingrid Brucknerova · Anna Holomanova · Mojmir Mach · Eduard Ujhazy
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    ABSTRACT: The paper highlights the personality of the founder of European student exchange program ERASMUS (EuRopean Community Action Scheme for the Mobility of University Students) Erasmus of Rotterdam. He was one of the leading European humanists and has left a literary legacy of large dimensions. His thoughts, ideas, opinions, and mainly the works have a great benefit for society even today. From 16th century to the present time they are the subject of unchanged interest.
    Neuro endocrinology letters 01/2013; 34(6):501-3. · 0.94 Impact Factor
  • Ingrid Brucknerova · Anna Holomanova
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    ABSTRACT: The paper highlights the personality of the founder of modern anatomy, who was able to use his knowledge and skills to change the view on the construction of the human body extending over centuries. He introduced a new scientific approach and highlighted the importance of autopsies for understanding of human body which carefully demonstrated and documented. De humani corporis fabrica - the spectacular work, in which he summarized results of his theoretical and practical findings, has opened a new path for the study of anatomy. Andreas Vesalius became a pioneer in the history of medical education. In 2014 will pass 500 years since his birth.
    Neuro endocrinology letters 01/2013; 34(6):498-500. · 0.94 Impact Factor
  • Ingrid Brucknerova · Anna Holomanova · Eduard Ujhazy · Mojmir Mach
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    ABSTRACT: It is well known that the names of discoverers are commonly used in medical terminology, especially in everyday use. The advantage of an eponym is that a complex description of a certain syndrome, surgical procedure, manifestations of a particular disease, or its details, can be expressed in a single word (Holomáňová & Brucknerová 2003). Current official anatomical nomenclatures do not use eponyms. Some exceptions do however exist. In pediatrics, we frequently use the names of two famous persons: Virginia Apgar and Ernst Moro.
    Neuro endocrinology letters 12/2012; 33(8). · 0.94 Impact Factor
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    ABSTRACT: Teratology is the science that studies the causes, mechanisms, and patterns of abnormal development. The authors present an updated overview of the most important milestones and stages of the development of modern teratology. Development of knowledge and society led to the recognition that causes of congenital developmental disorders (CDDs) might be caused by various mechanical effects, foetal diseases, and retarded or arrested development of the embryo and foetus. Based on the analysis of the historical development of hypotheses and theories representing a decisive contribution to this field, we present a survey of the six Wilson's fundamental principles of teratology. The aim of observing these principles is to get insight into developmental relations and to understand mechanisms of action on the level of cell populations (elementary morphogenetic processes), tissues and organs. It is important to realise that any negative intervention into the normal course of these processes, either on genetic or non-genetic basis, inevitably leads to a sequence of subsequent changes resulting in CDDs. Moreover, the classical toxicologic monotonic dose-response paradigm recently has been challenged by the so-called "low dose-hypothesis", particularly in the case of endocrine active substances. These include some pesticides, dioxins, polychlorobiphenyls (PCBs), and bisphenol A. Despite modern approaches of molecular biology and genetics, along with top diagnostic techniques, we are still not able to identify the actual cause in more than 65 to 70% of all congenital defects classified as having an unknown etiology. Today CDDs include any birth defect, either morphological, biochemical, or behavioural.
    Interdisciplinary toxicology 12/2012; 5(4):163-8. DOI:10.2478/v10102-012-0027-0
  • Ingrid Brucknerova · Anna Holomanova · Mojmir Mach · Eduard Ujhazy
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    ABSTRACT: The aim of this study is to present a short biography of some important physicians and describe the most prominent differences between trisomy 13, 18 and 21. The authors present the most prominent differences between trisomy 13, 18 and 21. The work of many important physicians, geneticists, has helped in the process of recognition of congenital anomalies. This group of famous persons includes Patau, Edwards and Down.
    Neuro endocrinology letters 12/2012; 33(7). · 0.94 Impact Factor
  • T Zikavska · I Brucknerova · O Cervenova · M Vidiscak · L Hustavova
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    ABSTRACT: There are many causes of intestinal obstruction in the neonatal age. The most common types are mechanical and result from congenital malformations of the gastrointestinal tract. However, functional disorders also occur. In some cases, diagnosis can be made prenatally but in others manifestation occurs after birth. The aim of this article is to present the case of a newborn with intestinal obstruction characterized by microcolon, dilated small bowel and megacystis known as Megacystis-Microcolon-Intestinal Hypoperistalsis syndrome, a very rare cause of intestinal obstruction. So far, less than three hundred cases have been reported in the literature. The prognosis of this syndrome is generally poor with most affected children dying in the neonatal period or infancy. Intestinal obstruction is a serious and life threatening condition. It may be explained by gross anatomical, histological or other abnormalities affecting the gut of the fetus. Differential diagnosis may also take into account various anatomical, histological, or other abnormalities that may result in the obstruction of gut. The incidence of intestinal obstruction is estimated at about one in 1500 live births.2 The cause of obstruction can be extrinsic or intrinsic. Atresia is quite common in the anorectal region, while atresia of the stomach occurs rarely. Colonic atresia is a rare condition of bowel obstruction in neonates. Membranous or complete obstruction may be present in the small intestine and may affect multiple sites. Stenosis may result from extrinsic or intrinsic factors. It arises from the failure of physiological rotation or fixation of the intestine in utero. Malrotation is one of the most serious cases, when there is incomplete rotation of the intestine into flexura duodenojejunalis or incomplete rotation of the caecum. Duodenal obstruction may also result when adhesion bands of Ladd straps down the second part of the duodenum. The second group of causes of bowel obstruction in the newborn consists of functional intestinal obstructions. It develops due to defects of bowel inervation and differentiation of ganglion cells. It includes small left colon syndrome or neuronal intestinal dysplasia type A and neuronal intestinal dysplasia type B.
    Nigerian journal of paediatrics 08/2012; 39(4). DOI:10.4314/njp.v39i4.12
  • T Zikavska · I Brucknerova · O Cervenova · V Bzduch
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    ABSTRACT: Errors of cholesterol biosynthesis represent a heterogeneous group of metabolic disorders. The aim of the authors of this article is to present a case of a patient with typical symptoms of a rare post-squalene disorder of cholesterol biosynthesis, its diagnostics and progress in neonatal period. The differential diagnosis of a typical findings on the skin with spontaneous regression ichtyosiform erythroderma, craniofacial dysmorphic features, anomalies of organs or skeletal abnormalities in a newborn may also be the result of a disorder of cholesterol biosynthesis. The final diagnosis is definitely confirmed by DNA analysis. Prognosis depends on the different enzyme defects of cholesterol biosynthesis pathway, but typically on the post-squalene pathway. Cholesterol is an important substance that plays a significant role in membrane structure, as well as being the precursor for the synthesis of the steroid hormones and bile acids. Cholesterol synthesis occurs in the cytoplasm and microsomes from the two-carbon acetate group of acetyl coenzyme A (acetyl-CoA). The biosynthesis of cholesterol consists of several reactions. Acetyl-CoA units are converted to mevalonate by a series of reactions. Mevalonate is formed on squalene and then lanosterol. Lanosterol is converted by two different pathways, either with the creation of 7-dehydrocholesterol, or desmosterol with the creation of cholesterol. Errors of cholesterol biosynthesis represent a heterogeneous group of metabolic disorders that is characterized by multiple dysmorphic features underlining an important role for cholesterol in human embryogenesis and development. The differential diagnosis of atypical findings on the skin in newborn may be the result of disorder of cholesterol biosynthesis. It may be also associated with various dysmorphic features or anomalies including multiple anomalies of congenital and internal organs and skeletal abnormalities. Some of the post-squalene disorders may point to atypical findings on the skin in the form of psoriatic eruptions, psychomotoric delay or laboratory findings as hypocholesterolemia.
    Nigerian journal of paediatrics 12/2011; 39(1). DOI:10.4314/njp.v39i1.6
  • E. Ujhazy · M. Dubovicky · J. Navarova · I. Brucknerova · M. Mach
    Fuel and Energy Abstracts 08/2011; 205. DOI:10.1016/j.toxlet.2011.05.866
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    ABSTRACT: The present study deals with effect of prenatal and neonatal administration of the synthetic pyridoindole derivative SMe1EC2 (2-ethoxycarbonyl-8-methoxy-2,3,4,4a,5,9b-hexahydro-1H-pyrido-[4,3b] indolinium chloride) on postnatal and neurobehavioral development of the rat offspring. The substance tested was administered to pregnant rats orally in the doses 5, 50 and 250 mg/kg from day 15 of gestation to day 10 post partum (PP). From the day 4 PP, the postnatal development and neurobehavioral characteritics of offspring were evaluated. The following variables were observed: body weight, pinna detachment, incisor eruption, ear opening, eye opening, testes descent and vaginal opening, righting reflex, negative geotaxia, startle reflex, dynamic air righting and exploratory behavior in a new environment. No maternal death, abortion or dead fetuses occurred either in the control or SMe1EC2 groups. Dynamic righting reflex was delayed one day in the groups of animals treated via their mothers with 5 and 50 mg/kg SMe1EC2. The delay in the development of this reflex was only transient. On day 20 PP, all pups tested had a positive score of the reflex. Administration of SMe1EC2 did not reveal any significant changes in other variables of somatic growth and maturation, reflex and neuromotor development and exploratory behavior, either of young or adult animals of both genders, assessed by analysis of variance.
    Interdisciplinary toxicology 03/2011; 4(1):47-51. DOI:10.2478/v10102-011-0009-7
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    ABSTRACT: Asphyxia of the newborn has a varied etiology. Clinical consequences have a broad spectrum of presentations. Arteriovenous malformation associated with an aneurysm of the Galen vein can be the cause of focal ischemic changes in the nervous parenchyma. The authors report a case of a term newborn (birth weight 4, 000 grams, Apgar score 7/9). Physical investigation confirmed the presence of a continuous murmur in the area of the anterior fontanelle. Ultrasonic investigation of the brain detected a huge arteriovenous malformation of the Galen vein. Ultrasonic investigation of the heart excluded structural anomaly, but confirmed a huge retrograde flow in the aorta descendens, opened ductus arterious with suspected formation of coarctation of the aorta and dilatation of the vena cava superior. Congenital anomaly of the Galen vein has a negative influence on prenatal and postnatal development of the brain of a newborn. In the case of our patient, it led to rapid severe asphyxiated changes of the brain parenchyma. Diagnosis and management were established, yet endovascular therapy was not indicated in the early neonatal period.
    Neuro endocrinology letters 01/2011; 32 Suppl 1:5-7. · 0.94 Impact Factor
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    ABSTRACT: To present the case of a term newborn with rapid progression of signs of neurodegenerative disease. In a case of a term newborn with numerous dysmorphic features, with seizure activity from the 3rd day of life, hypertonia and serious changes on brain parenchyma were presented. Diagnosis of molybdenum cofactor deficiency was confirmed by the decreased level of uric acid, 31 μmol/l, in serum, increased excretion of thiosulfate and S-sulfocysteine in urine, taurine (1729.3 μmol/mmol crea; normal range 30-300 μmol/mmol crea) and xanthine (276.9 μmol/mmol crea; normal range < 25 μmol/mmol crea) in urine. Sulfite oxidase activity on skin fibroblasts in culture was not detectable. The patient died at the age of 28 days of life. Deficiency of molybdenum cofactor leads to accumulation of toxic metabolites (levels of sulfite), which causes disturbances of neurotransmitters even before delivery. Therapy is symptomatic, no effective therapy is available. Seizures are difficult to suppress. This case report is about the first patient in Slovakia.
    Neuro endocrinology letters 12/2010; 31 Suppl 2(Suppl 2):5-7. · 0.94 Impact Factor

Publication Stats

58 Citations
25.86 Total Impact Points

Institutions

  • 2002–2015
    • Comenius University in Bratislava
      • Faculty of Medicine
      Presburg, Bratislavský, Slovakia
  • 2014
    • Detská fakultná nemocnica s poliklinikou v Bratislave
      Presburg, Bratislavský, Slovakia
  • 2006–2013
    • Slovak Academy of Sciences
      • • Institute of Experimental Pharmacology and Toxicology
      • • Department of Experimental Pharmacology
      Bratislava, Bratislavsky Kraj, Slovakia