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ABSTRACT: Recent studies have demonstrated that five subtypes (M1-M5) of muscarinic acetylcholine receptor (mAChR) are expressed in the vestibular periphery. However, the exact cellular location of the mAChRs is not clear. In this study, we investigated whether there is the expression of M1-M5 muscarinic receptor mRNA in isolated type II vestibular hair cells of guinea pig by using single-cell RT-PCR. In vestibular end-organ, cDNA of the expected size was obtained by RT-PCR. Moreover, mRNA was identified by RT-PCR from individually isolated type II vestibular hair cells (single-cell RT-PCR). Sequence analysis confirmed that the products were M1-M5 mAChR. These results demonstrated that M1-M5 mAChR was expressed in the type II vestibular hair cells of the guinea pig, which lends further support for the role of M1-M5 mAChR as a mediator of efferent cholinergic signalling pathway in vestibular hair cells.
Journal of Huazhong University of Science and Technology 10/2011; 31(5):682-6. · 0.38 Impact Factor
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ABSTRACT: To explore the proper diagnosis and therapy method in aged patients with bronchial foreign body.
A retrospective analysis was performed on 15 aged patients with bronchial foreign body by clinical characteristics. The experience of diagnosis and therapy in these cases were summarized.
Misdiagnosed or missed diagnosis were found in 6 of 15 aged patients with bronchial foreign body. Three bronchial foreign bodies were taken out successfully under topical anesthesia. Twelve bronchial foreign bodies were taken out under topical anesthesia and balanced anesthesia. No complications happened in all case.
Clinical manifestation of bronchial foreign body is often unrepresentative in most aged patients. The chest X-ray of most cases can not show actual appearance of foreign bodies. In addition, history of foreign body in bronchus is often uncertain. So bronchial foreign bodies have often been misdiagnosed or missed diagnosed in aged patients. Suitable anesthesia method is the key to taking out bronchial foreign body in aged patients.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2007; 21(8):359-60.
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ABSTRACT: To discuss the method of extracting nucleic acid from rat's hair.
The method with PCR buffer, SDS-proteinase K or Chelex-100 were used to extract the nucleic acid from rat's hair separately, and the isolated nucleic acid was analyzed by PCR and electrophoresis.
The success rate of antracting mt DNA from the hair gollcle, we gound the method with SDS-prot einase kis lower than the ather two methods (chi1(2) = 42.421, chi2(2) = 28.800, P<0.01). This is same as the result ot entracting nuclear DNA (chi1(2) = 49.091, chi2(2) = 30.767, P<0.01). While no alifference has been bound between the method with PCR buffer and with chelex-100 (mtDNA: chi(2) = 0.296; nuclear DNA: chi(2) = 0.048, P>0.05). The method with PCR buffer can't extract nucleic acid from hair shafts, the method with SDS-proteinase K can't extract mtDNA from one or two hairs, the method with Chelex-100 can extract mtDNA and nuclear DNA from single rat's hair or hair shafts.
The method with Chelex-100 is suitable for extracting nucleic acid from hair.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2004; 18(5):288-91.
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ABSTRACT: To evaluate the feasibility and value of integrated methods to assess auditory pathway integrity.
Twenty-four cases of bilateral profoundly-deafened individuals who were considered as the candidates of cochlear implantation were included in this study. Auditory pathway integrity from these candidates of cochlear implantation were assessed with the integrated methods established by our team, which consist of 5 categories including 1. audiological test; 2. radiological imaging study; 3. ear-canal electric audiometry; 4. response to sound in daily life; and 5. speech development.
Twenty-three candidates who meet the criteria of auditory nerve-auditory pathway integrity received cochlear implantation with improved hearing and speech development postoperatively. The remainder one of the 24 candidates was diagnosed as bilateral absence of auditory nerve. The fault of cochlear implantation was avoided.
The integrated methods for assessing auditory pathway integrity is feasible and valuable. Auditory nerve-auditory pathway integrity should be considered and included as one of the most important criteria for cochlear implantation candidate.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 01/2004; 17(12):705-8.
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ABSTRACT: To investigate the expression of the gamma-aminobutyric acid receptor gamma 1 subunit in the end-organs of rat vestibule.
Using a combination of reverse transcription followed by polymerase chain reaction, expression of the gamma-aminobutyric acid receptor gamma 1 subunit in the end-organs of rat vestibule was examined. The rat brain RNA was used as positive control.
PCR amplification products representing subunit gene expression for gamma-aminobutyric acid receptor alpha 1 subunit were amplified.
The results showed that gamma-aminobutyric acid receptor gamma 1 subunit was expressed in the rat vestibule, indicating that GABA is one of the important neurotransmitters in the vestibular system.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 05/2003; 17(4):229-30.
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ABSTRACT: To analyze the incidence of three types of mitochondrial DNA mutations in the non-syndromic sensorineural hearing loss (NSSNHL) patients and control subjects in order to investigate the possible role of mitochondrial DNA mutations in NSSNHL.
Sixty-one sporadic NSSNHL patients (from 3 to 84 years old) and 19 control subjects matched for age were selected. DNA was extracted from isolated blood leukocytes. Interrupt polymerase chain reaction (PCR) and primer-shift PCR were used to detect the mtDNA4977 deletion; mtDNA1555A-->G and mtDNA3243A-->G point mutation were detected by PCR and restriction fragment length polymorphism (RFLP) analysis. PCR products were sequenced by automated laser fluorescent DNA sequencer.
The detection rate of mtDNA4977 deletion in deafness groups and control groups are 68.85%(42/61) vs. 5.26%(1/19). Among all the samples, neither any mtDNA1555A-->G mutation nor mtDNA3243A-->G point mutation was detected.
MtDNA4977 deletion had a high detection rate in patients with NSSNHL. MtDNA1555A-->G mutation and mtDNA3243A-->G point mutation may not be common mutations in patients with NSSNHL.
Zhonghua er bi yan hou ke za zhi 10/2002; 37(5):338-42.
