Hua-Ming Yu

Publications (3)3.78 Total impact

• Article: Determination of trace bismuth by flow injection-hydride generation collection-atomic absorption spectrometry.

  Shu Yu Chen, Zhi Feng Zhang, Hua Ming Yu
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  ABSTRACT: Bismuth hydride gas was collected on-line and determined via a new flow injection-hydride generation collection-flame atomic absorption spectrometry system. The performance of the gas-liquid separator, hydride gas collection time, acidity of the sample solution, NaBH(4) concentration, and the effects of concomitant interferents were investigated to optimize the conditions of this new method. Interferences from concomitant elements were investigated, and recoveries of 94.7-105.3% for 10 ng mL(-1) Bi were obtained after the addition of 0.2% ascorbic acid-thiourea masking reagents. The sensitivity of this new method was one order of magnitude higher than the continuous flow-hydride generation-flame atomic absorption method with a detection limit of 0.25 ng mL(-1) and a precision of 2.3%. The method was evaluated by determining trace bismuth in standard biological reference material human hair GBW07601, and the results were consistent with the certified value. The proposed method was then employed to determine trace bismuth in ten colored gelatin samples; recoveries of 94.2-105.8% were obtained.
  Analytical and Bioanalytical Chemistry 10/2002; 374(1):126-30. · 3.78 Impact Factor
• Article: Fluorescence Analysis of the Fibrinolytic Principle (FP) from Agkistrodon acutus Venom.

  Shou-Ye Wang, Hua-Ming Yu, Zhen-Long Chen, Zu-De Zhang, Shuang-Ding Wu, Qing-Liang Liu
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  ABSTRACT: The conformation and the properties of the fibrinolytic principle (FP) from Agkistrodon acutus venom were studied by chemical modification and fluorescence spectroscopy. Results showed that there are more than one tryptophan (Trp) residue in the FP molecule and they are located in the more hydrophobic core, could be quenched by acrylamide (Acr), a polarized quencher without electric charge. The collisional quenching constants of FP at different concentrations of Acr were calculated in terms of Stern-Volmer equation, and the fraction of the Trp quenched was obtained by the modified Stern-Volmer equation as 83%.
  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 02/1998; 30(3):303-306.
• Article: Biochemical Properties of Bound-Ca(2+) in Fibrinolytic Principle (FP) Separated from Agkistrodon acutus Venom.

  Shou-Ye Wang, Zu-De Zhang, Zhen-Long Chen, Hua-Ming Yu, Shuang-Ding Wu, Qing-Liang Liu
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  ABSTRACT: It has been determined that each FP molecular contains one Ca(2+)-binding site. By the use of fluorescence probe Tb(3+), the distance between Tb(3+) and tryptophan (Trp) residue was obtained to be 0.375. Tb(3+) ion is coordinated with FP more strongly than Ca(2+) ion, and can bind to FP and replace the Ca(2+) ion in FP completely.
  Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 02/1998; 30(3):299-302.