Publications (23)28.2 Total impact
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Article: [Analysis of chemical constituents of Chuanxiong Rhizoma absorbed into rat brain tissues by UPLC-Q-TOF-MS].
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ABSTRACT: To analyze and identify the chemical constituents in rat brain tissues after oral administration of Chuanxiong Rhizoma extracts. The dosed and blank rat brain tissues were analyzed by UPLC-Q-TOF-MS. Different peaks were observed in total ion chromatograms and then identified according to their retention time, accurate mass weight, MS and MS/MS data. After oral administration of Chuanxiong Rhizoma extracts, 3 compounds were absorbed into rat brain tissues through BBB. They were identified as senkyunolide I, senkyunolide A and ligustilide. The study is helpful for interpreting effective substance of Ligusticum chuanxiong.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 12/2012; 37(23):3647-50. -
Article: [Research progress studies on pharmacology and pharmacokinetics of ligustilide].
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ABSTRACT: Ligustilide is contained highly or around 1% in such umbelliferous plants as Angelica sinensis and Ligusticum chuanxiong, is one of main bioactive constituents. It shows many pharmacological activities related to their efficacy. At present, ligustilide has attracted extensive attention and more and more studies have been reported, indicating that it is a promising compound. This essay summarizes the progress of pharmacological effects of ligustilide on neuroprotection, vasodilatation, anti-caner and anti-tumor, analgesia and anti-inflammation, and pharmacokinetics including absorption, distribution, metabolism and excretion, providing basis for further studies and development of ligustilide.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 11/2012; 37(22):3350-3. -
Article: [Screening of bioactive components in epimedium for osteoporosis treatment by model population analysis].
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ABSTRACT: Epimedium was obtained from different habitats, and their bioactive components for inhibiting RAW264.7 were screened by MTT assay. Results indicate that epimedium from different habitats displayed significant different activities. By means of model population analysis (MPA), a latent bioactive component, baohuoside-I was got. Activity of baohuoside-I wasvalidated and prior to icariin. MPA can be used for bioactive components screening.Yao xue xue bao = Acta pharmaceutica Sinica 09/2012; 47(9):1205-9. -
Article: Two new phenolic glycosides from the rhizome of Gastrodia elata.
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ABSTRACT: Two new phenolic glycosides, named parishins F-G (1-2), together with known parishin E, were isolated from the rhizome of Gastrodia elata. The new structures were established as 1,3-di-[4-O-(β-D-glucopyranosyl) benzyl]-2-{4-O-[β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranosyl] benzyl} citrate (1) and 2-[4-O-(β-D-glucopyranosyl)benzyl] citrate (2), by means of MS, 1D, and 2D NMR spectral analyses, as well as chemical methods.Journal of Asian natural products research 03/2012; 14(5):457-62. · 0.61 Impact Factor -
Article: Taxol-induced alteration of intracellular amino-acid profile related to human cervical carcinoma HeLa cell death.
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ABSTRACT: The anti-tumor action of Taxol was investigated in the changes of amino-acids involved in tumor cell survival. By tracing the intracellular amino-acid profiles of HeLa cells treated with non-conditioned and three conditioned media (Taxol, L-alanine, and Taxol + L-alanine), it was observed that an alteration of amino-acid metabolism participates in Taxol-induced death of HeLa cells. The contents of 18 out of 21 detected amino-acids are 5-95% and the ones of lysine and methionine are 158 and 117% of the corresponding contents in the control after treatment with Taxol for 24 h, respectively. Addition of L-alanine inhibited cell apoptosis upon Taxol treatment by partially blocking the increase of lysine and methionine and reversing decrease trend of alanine, glycine, and glutamic acid. These results suggest that interference of amino-acid metabolism might be an important mechanism of Taxol cytotoxicity.Biotechnology Letters 09/2011; 34(1):1-7. · 1.68 Impact Factor -
Article: A new diterpenoid from Rhizophora apiculata.
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ABSTRACT: A new diterpenoid, 15(S)-isopimar-7-en-1-oxo-15,16-diol (1), was isolated from the stems of mangrove plant Rhizophora apiculata. The structure of the new compound was elucidated by MS, IR, 1D, and 2D NMR techniques, including HMQC, HMBC, and NOESY correlations. In addition, seven known constituents were isolated from this plant for the first time.Journal of Asian natural products research 08/2011; 13(8):776-9. · 0.61 Impact Factor -
Article: Bioactive compounds from the aerial parts of Brachystemma calycinum and structural revision of an octacyclopeptide.
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ABSTRACT: Four new cyclic peptides, brachystemins F-I (1-4), and 11 known compounds were isolated from the aerial parts of Brachystemma calycinum. The absolute configurations of compounds 1-4 were assigned using Marfey's method. The structure of compound 5 was revised from cyclo(Pro¹-Phe²-Leu³-Ala⁴-Thr⁵-Pro⁶-Ala⁷-Gly⁸) to cyclo(Pro¹-Pro²-Ala³-Gly⁴-Leu⁵-Ala⁶-Thr⁷-Phe⁸) with QTOF/MS and X-ray diffraction analysis. The N-containing compounds were assessed for their inhibitory effects on the secretion of monocyte chemokine ligand 2 (CCL-2), interleukin 6 (IL-6), and collagen IV against high-glucose-stimulated mesangial cells. Compound 5 was evaluated for its effects on collagen I, reactive oxygen species (ROS), superoxide anion (O₂(•⁻)) production, and cell viability in mesangial cells, and on nitric oxide (NO) production in macrophage cells.Journal of Natural Products 06/2011; 74(6):1392-400. · 3.13 Impact Factor -
Article: Rhizoma coptidis and berberine-induced activation of murine microglia N9 cells.
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ABSTRACT: To investigate the effect of water extract of Rhizoma coptidis (WEC) and berberine on the activation of murine microglia N9 cells and corresponding mechanism related to mitochondria. Phagocytic activity of murine microglia N9 cells was measured by neutral red staining method after the cells were treated with various concentrations of WEC and alkaloids for 24h. Flow cytometric analysis was performed to determine the level of intracellular ROS, Ca(2+), and mitochondrial transmembrane potential (Delta psi) after 87 microg/ml of WEC and 12.4 microg/ml of berberine treatment. Global changes of gene expression in WEC- and berberine-treated N9 cells were measured using cDNA microarray. WEC and berberine, but not palmatine and jatrorrhizine, enhanced phagocytic activity of murine N9 cells in a dose-dependent manner. Both of WEC and berberine stimulated free radical generation, enhanced mitochondrial Delta psi and induced gene expression of Ndufab1, Cox6a2 and Atp5a1. However, a more significant phagocytic effect was observed for WEC. WEC, but not berberine, increased intracellular Ca(2+) concentration. The gene expression of Atp5c1 was selectively up-regulated by WEC, while three genes of Uqcrq, Cox8b, and Atp5g2 were induced by berberine. WEC and berberine activated murine microglia N9 cells by the regulation of mitochondrial function and mitochondria-related signal molecules. The action of WEC is stronger than that of berberine, indicating that the effect of WEC is ascribed partially, but not totally, to berberine.Journal of ethnopharmacology 03/2010; 129(1):121-6. · 2.32 Impact Factor -
Article: Taxane's substituents at C3' affect its regioselective metabolism: different in vitro metabolism of cephalomannine and paclitaxel.
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ABSTRACT: To investigate how taxane's substituents at C3' affect its metabolism, we compared the metabolism of cephalomannine and paclitaxel, a pair of analogs that differ slightly at the C3' position. After cephalomannine was incubated with human liver microsomes in an NADPH-generating system, two monohydroxylated metabolites (M1 and M2) were detected by liquid chromatography/tandem mass spectrometry. C4'' (M1) and C6alpha (M2) were proposed as the possible hydroxylation sites, and the structure of M1 was confirmed by (1)H NMR. Chemical inhibition studies and assays with recombinant human cytochromes P450 (P450s) indicated that 4''-hydroxycephalomannine was generated predominantly by CYP3A4 and 6alpha-hydroxycephalomannine by CYP2C8. The overall biotransformation rate between paclitaxel and cephalomannine differed slightly (184 vs. 145 pmol/min/mg), but the average ratio of metabolites hydroxylated at the C13 side chain to C6alpha for paclitaxel and cephalomannine varied significantly (15:85 vs. 64:36) in five human liver samples. Compared with paclitaxel, the major hydroxylation site transferred from C6alpha to C4'', and the main metabolizing P450 changed from CYP2C8 to CYP3A4 for cephalomannine. In the incubation system with rat or minipig liver microsomes, only 4''-hydroxycephalomannine was detected, and its formation was inhibited by CYP3A inhibitors. Molecular docking by AutoDock suggested that cephalomannine adopted an orientation in favor of 4''-hydroxylation, whereas paclitaxel adopted an orientation favoring 3'-p-hydroxylation. Kinetic studies showed that CYP3A4 catalyzed cephalomannine more efficiently than paclitaxel due to an increased V(m). Our results demonstrate that relatively minor modification of taxane at C3' has major consequence on the metabolism.Drug metabolism and disposition: the biological fate of chemicals 03/2008; 36(2):418-26. · 3.74 Impact Factor -
Article: Functional analysis of cultured neural cells for evaluating cold/cool- and hot/warm-natured Chinese herbs.
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ABSTRACT: Recently, modern scientific research has been required to understand pharmacological basis of traditional Chinese medicine (TCM) theory based on the ancient clinical experience, and to investigate the molecular mechanisms of action of Chinese herbs. Here, 20 Chinese herbs, classified into 4 properties (hot, warm, cold and cool) of TCM, were analyzed for their ability to exhibit antioxidant action, to enhance glucose uptake by murine microglia N9 cells, and to influence neurotransmitter norepinephrine (NE) release from rat pheochromocytoma PC12 cells. We found a generally protective effect of both hot/warm-natured and cold/cool-natured herbs against H(2)O(2)-induced N9 cell death, partially by elevating superoxide dismutase (SOD) activity. Glucose uptake was elevated after treatment with some hot/warm-natured herbs. In addition, most herbs with hot/warm nature tended to stimulate NE release, while such stimulatory effect was not observed in the herbs with cold/cool nature. Two cold/cool-natured herbs, Rhizoma coptidis and Radix scutellariae, even significantly suppressed the release. These results suggest that the distinct abilities of Chinese herbs to regulate neural cell functions appear to be correlated with their natures identified in traditional TCM theory, and may be a useful guide for their utility in neural degenerative diseases.The American Journal of Chinese Medicine 02/2008; 36(4):771-81. · 1.98 Impact Factor -
Article: Simultaneous characterization of isoflavonoids and astragalosides in two Astragalus species by high-performance liquid chromatography coupled with atmospheric pressure chemical ionization tandem mass spectrometry.
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ABSTRACT: A method was developed for the simultaneous identification of astragalosides (AGs) and isoflavonoids (IFs) in the roots of Astragalus membranaceus and Astragalus mongholicus by HPLC coupled with atmospheric pressure chemical ionization MS/MS (HPLC-APCI-MS/MS). Diagnostic fragment ions of AGs and different group of IFs were obtained with one AG and eight IF standards analyzed by CID-MS, which were adopted as characteristic MS/MS fingerprints for further identification of these compounds in the two Astragalus species by using HPLC-APCI-MS/MS. A total of 20 IFs and 10 AGs were identified or tentatively identified. Among them, six IFs were detected in A. membranaceus for the first time and five IFs were firstly identified in A. mongholicus. The results indicate that HPLC-APCI-MS/MS is a powerful tool for the simultaneous characterization of IFs and AGs in complex matrix.Journal of Separation Science 09/2007; 30(13):2059-69. · 2.73 Impact Factor -
Article: Kinetics and mechanism of degradation of lithospermic acid B in aqueous solution.
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ABSTRACT: The degradation of lithospermic acid B (LAB) was investigated as a function of buffer concentration, pH and temperature. Stability tests were performed using a stability-indicating high-performance liquid chromatography (HPLC) with UV-vis detection. The degradation followed pseudo-first-order kinetics under all experimental conditions. The maximum stability of LAB was observed at pH 2.0. The logk(pH)-pH profile described by specific acid-base catalysis and water molecules agreed with the experimental results. The overall degradation rate constant as a function of the temperature under the given conditions obeyed the Arrhenius equation. The chemical fate of LAB in mild acidic solution was investigated, and nine degradation products were detected and tentatively identified by LC-MS analysis. The primary degradation pathway involving the cleavage of ester bond and ring-opened of benzofuran in the LAB are proposed.Journal of Pharmaceutical and Biomedical Analysis 04/2007; 43(4):1249-55. · 2.97 Impact Factor -
Article: Hydrolytic kinetics of lithospermic acid B extracted from roots of Salvia miltiorrhiza.
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ABSTRACT: The hydrolytic kinetics of lithospermic acid B (LAB) extracted from the roots of Salvia miltiorrhiza (Chinese herb: danshen) was investigated by using reversed-phase high-performance liquid chromatography (HPLC) with UV-vis detection. The influences of initial drug concentration, pH and temperature on hydrolysis of LAB were studied in aqueous solutions. The results showed that initial concentration of LAB has no effect on the degradation rate at pH 2.0. The hydrolysis followed pseudo-first-order kinetics at 90 degrees C. The log k(obs)-pH profile indicated that the optimal stability range was at pH 2.0-5.0. The rate constant of overall hydrolysis as a function of temperature under the given conditions obeyed the Arrhenius equation. Analysis of the acid-induced degraded solution of LAB by liquid chromatography-mass spectrometry (LC-MS) revealed at least four degradation products [M-H](-) ion at m/z 197, 137, 537 and 537, respectively. Three of these degradation products, i.e. danshensu (DSU), protocatechuic aldehyde (PRO), and lithospermic acid, were further identified by comparing the retention times with standard samples. According to the structure of LAB and its hydrolysis behavior in solution, the other product was proposed to be the isomer of lithospermic acid.Journal of Pharmaceutical and Biomedical Analysis 02/2007; 43(2):435-9. · 2.97 Impact Factor -
Article: Xanthone glycosides from Swertia franchetiana.
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ABSTRACT: A new xanthone glycoside (1) has been isolated from Swertia franchetiana together with five known xanthone glycosides. Their structures were elucidated as 7-O-[beta-D-xylopyranosyl-(1-->2)-beta-D-xylopyranosyl]-1,7,8-trihydroxy-3-methoxyxanthone (1), 7-O-[alpha-L-rhamnopyranosyl-(1-->2)-beta-D-xylopyranosyl]-1,7,8-trihydroxy-3-methoxyxanthone (2), 8-O-beta-D-glucopyranosyl-1,3,5,8-tetrahydroxyxanthone (3), 1-O-beta-D-glucopyranosyl-1-hydroxy-3,7,8-trimethoxyxanthone (4), 1-O-[beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranosyl]-1-hydroxy-2,3,5-trimethoxyxanthone (5) and 1-O-[beta-D-xylopyranosyl-(1-->6)-beta-D-glucopyranosyl]-1-hydroxy-3,5-dimethoxyxanthone (6) on the basis of spectroscopic evidence.Journal of Asian Natural Products Research 05/2005; 7(2):175-9. · 0.94 Impact Factor -
Article: Note
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ABSTRACT: A new xanthone glycoside ( 1 ) has been isolated from Swertia franchetiana together with five known xanthone glycosides. Their structures were elucidated as 7- O -[- d -xylopyranosyl-(12)-- d -xylopyranosyl]-1,7,8-trihydroxy-3-methoxyxanthone ( 1 ), 7- O -[- l -rhamnopyranosyl-(12)-- d -xylopyranosyl]-1,7,8-trihydroxy-3-methoxyxanthone ( 2 ), 8- O- - d -glucopyranosyl-1,3,5,8-tetrahydroxyxanthone ( 3 ), 1- O- - d -glucopyranosyl-1-hydroxy-3,7,8-trimethoxyxanthone ( 4 ), 1- O -[- d -xylopyranosyl-(16)-- d -glucopyranosyl]-1-hydroxy-2,3,5-trimethoxyxanthone ( 5 ) and 1- O -[- d -xylopyranosyl-(16)-- d -glucopyranosyl]-1-hydroxy-3,5-dimethoxyxanthone ( 6 ) on the basis of spectroscopic evidence.Journal of Asian Natural Products Research 03/2005; 7(2):175-179. · 0.94 Impact Factor -
Article: A new amide from Asarum forbesii Maxim.
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ABSTRACT: A highly unsaturated new amide, (2E,4Z,8Z,10Z)-N-isobutyl-2,4,8,10-dodecatetraenamide (1), was isolated in very small quantities from the whole plant of Asarum forbesii Maxim. together with four known compounds, (2E,4E,8Z,10E)-N-isobutyl-2,4,8,10-dodecatetraenamide (2), (-)-sesamin (3), (-)-asarinin (4) and (E)-asarone (5). The Z/E isomers, 1 and 2, were separated successfully by developed silver-ion medium-pressure liquid chromatography (SIMPLC). Compound 2 and the two diastereoisomers, 3 and 4, were isolated from this plant for the first time. The characterization of these compounds was achieved by various spectroscopic methods.Journal of Asian Natural Products Research 03/2005; 7(1):1-5. · 0.94 Impact Factor -
Article: [Study on GC fingerprint of the constituents in Herba Asari].
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ABSTRACT: To establish a method for GC fingerprint determination of the chemical constituents in Herba Asari. GC and GC-MS were used to optimize the fingerprint determination method, and identify the main peaks in the GC fingerprint. A preferable method for GC fingerprint determination of the chemical constituents in Herba Asari was established. A general acquaintance of the chemical constituents in Herba Asari can be obtained by using the preferable GC fingerprint determination method, which is useful for quality evaluation of the crude drug of Herba Asari.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 06/2004; 29(5):411-3. -
Article: Pharmacokinetic study on spinosin in rat plasma after oral administration of suanzaoren extract at a single dose.
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ABSTRACT: To study the pharmacokinetics of spinosin in rat plasma after oral administration of Suanzaoren extract using sulfamethoxazole (SMZ) as internal standard by RP-HPLC method. Plasma samples were deproteined with acetonitrile, followed by evaporation of the acetonitrile to dryness. The residual was then resolved in mobile phase and HPLC separation was achieved on a Hypersil C18 (5 microns, 200 mm x 4.6 mm ID) column at 35 degrees C. The mobile phase consisted of acetonitrile-water-acetic acid (15:85:1) at a flow rate of 0.7 mL.min-1. The UV detection wavelength was set at 334 nm. The calibration curve was shown to be linear over the range from 18.1 to 903.5 micrograms.L-1 (r2 > or = 0.995). Mean recovery was 94.5%. Within-day and between-day precisions RSD were less than 9.0%. The limit of quantitation was 18.1 micrograms.L-1. The plasma spinosin was stable at -20 degrees C. The simple, sensitive and accurate HPLC method developed has been applied to determine the pharmacokinetics of spinosin in rat plasma after having taken Suanzaoren extract at a single dose.Yao xue xue bao = Acta pharmaceutica Sinica 06/2003; 38(6):448-50. -
Article: [Determination of hesperidin and naringin in Fructus Aurantii Immaturus and Fructus Aurantii by reversed-phase high performance liquid chromatography].
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ABSTRACT: An HPLC method was established for the determination of hesperidin and naringin in the Fructus Aurantii Immaturus and Fructus Aurantii from different places. Hesperidin and narigin were separated on a Hypersil ODS1 column with acetonitrile-0.5% acetic acid (volume ratio of 22:78) as the mobile phase and detected at 283 nm. The flow rate was 1.0 mL/min. The column temperature was set at 35 degrees C. The calibration curves were linear in the ranges of 0.060 g/L-0.604 g/L and 0.0125 g/L-0.125 g/L for naringin and hesperidin respectively. The average recoveries were 97.1% for naringin and 95.3% for hesperidin. The method is simple, rapid, accurate and reliable.Se pu = Chinese journal of chromatography / Zhongguo hua xue hui 12/2002; 20(6):585-6. -
Article: [Vectorial angle method for evaluating the similarity between two chromatographic fingerprints of Chinese herb].
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ABSTRACT: To evaluate the similarity between two chromatographic fingerprints automatically with computer. Chromatogram can be treated as vector of hyperspace, and the similarity between them can be counted according to vectorial angle formula. This process was performed with software written in Visual Basic 6.0. The two main functions of this software are automatic peak tracking in two fingerprints under the same analytic condition and computing the similarity automatically. The HPLC fingerprints of eleven kinds of Evodia rutaecarpa (Juss.) Benth (a traditional Chinese herb) from different sources were obtained and the similarities were calculated with this software. This method was shown to be a good way to evaluate the similarity between two fingerprints. A sample washed seven times with hot water can be clearly discriminated from other samples of Evodia rutaecarpa (Juss.) Benth with similar results. This method is a good way to evaluate the similarity between two fingerprints and is helpful in quality control of traditional Chinese medicine.Yao xue xue bao = Acta pharmaceutica Sinica 10/2002; 37(9):713-7.
Top co-authors
Top Journals
Institutions
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2012
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Dalian Institute of Chemical Sciences
Dalian, Liaoning, China
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2004–2011
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Chinese Academy of Sciences
- Laboratory of Analytical Chemistry for Life Science
Beijing, Beijing Shi, China
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2007
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Dalian University of Technology
- Department of Bioscience and Biotechnology
Dalian, Liaoning, China
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