L I Gunderina

Publications (19)4.36 Total impact

• Article: Complexity decompositions in the problem of comparison of polytene chromosome banding sequences

  L. A. Miroshnichenko, V. D. Gusev, I. I. Kiknadze, L. I. Gunderina, A. G. Istomina
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  ABSTRACT: The computation aspects of the problem of phylogenetic tree construction based on unique data of polytene chromosome banding sequences were considered. The possibilities of exact and approximate calculation of inversion distances were researched. The phylogenetic trees of relationships between 65 species of genus Chironomus (non-biting midge) were built and analyzed.
  Pattern Recognition and Image Analysis 05/2012; 21(3):534-537.
• Article: Chromosomal variability in natural populations of Chironomus cingulatus meigen (Diptera, Chironomidae)

  A. G. Istomina, I. I. Kiknadze, L. I. Gunderina
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  ABSTRACT: Chromosomal polymorphism has been studied in seven natural populations of Chironomus cingulatus from Western Europe, Western Siberia, and the Republic of Sakha (Yakutia). The banding sequences pool of the species includes 15 banding sequences. Chromosomal polymorphism was revealed in five out of seven chromosomal arms. Arm B is the most polymorphic with four banding sequences. There are three banding sequences in arm A. Arms D, E, and G have two banding sequences. None of the chromosome rearrangements were revealed in arms C and F. The populations of C. cingulatus differ clearly in their number and frequency of banding sequences, which indicates that different gene sequences are adaptive in different populations.
  Cell and Tissue Biology 04/2012; 3(2):149-161.
• Article: Divergence of genomic DNA in species of the subgenus Camptochironomus (Diptera, Chironomidae) differing in their cytogenetic similarity levels

  L. I. Gunderina, L. I. Kiknadze, A. G. Istomina, M. Butler
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  ABSTRACT: The variation and divergence of genomic DNA in four species of the subgenus Camptochironomus (C. tentans, C. dilutus, C. pallidivittatus, and C. setivalva) differing in the level of their cytological similarity were analyzed using the RAPD (Randomly Amplified Polymorphic DNA) method. A high level of variation in the RAPD markers was found in the species studied. Genetic distances (GD) were assessed between natural C. tentans populations, between different species of the camptochironomus sibling species group (C. tentans, C. dilutus, and C. pallidivittatus), and between these species and C. setivalva which is outside this sibling species group. The GD values obtained characterize the levels of genomic differentiation among natural populations (GD = 0.248), among sibling species (GD = 0.635), and between incipient species (GD = 0.784) of the subgenus Camptochironomus. The degree of genomic DNA divergence between sibling and incipient species in the subgenus Camptochironomus was found to be lower than that in the genus Chironomus. The rate of genomic DNA divergence appears to be lower than the rate of chromosomal divergence in species of the subgenus Camptochironomus.
  Entomological Review 04/2012; 87(8):935-946.
• Article: Differentiation of chromosome banding sequence pools and genomic dna in Holarctic natural populations of Chironomus entis Shobanov (Diptera, Chironomidae)

  L. I. Gunderina, I. I. Kiknadze, V. V. Golygina, M. G. Butler
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  ABSTRACT: Polymorphism and differentiation of the chromosome banding sequence pools and genomic DNA were studied in three natural populations of Chironomus entis from Europe and North America. These populations showed a moderate level of chromosomal polymorphism and high RAPD polymorphism of genomic DNA. The Palearctic and Nearctic populations of this species did not differ significantly in the levels of chromosome and genomic DNA polymorphism. Estimation of the cytogenetic (GDcg) and genetic (GDDNA) distances between these C. entis populations showed that their chromosome banding sequence pools and cytogenetic structures are differentiated to a greater extent than genomic DNA. The values of cytogenetic and genetic distances between the Palearctic and Nearctic populations of C. entis are higher than the values of the corresponding distances between the Nearctic populations, but they do not reach the level of divergence between species.
  Russian Journal of Genetics 04/2012; 45(12):1413-1421. · 0.43 Impact Factor
• Article: Geographic differentiation of genomic DNA of Chironomus plumosus (Diptera, Chironomidae) in natural holarctic populations

  L. I. Gunderina, I. I. Kiknadze, A. G. Istomina, M. Butler
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  ABSTRACT: Using RAPD markers, polymorphism and differentiation of genomic DNA was examined in seven natural populations of Chironomus plumosus from Europe, Siberia, and North America. All these populations showed high polymorphism of genomic DNA. The Palearctic and Nearctic populations of this species were not statistically significantly different in the genomic DNA polymorphism level. The genetic distance (GD), which characterizes the extent of intraspecific differentiation of population genetic structure, was determined among the natural populations of C. plumosus. The genetic distance was on average 0.245. It was demonstrated that genetic structures of the Palearctic and Nearctic populations of C. plumosus was differentiated to a higher extent than in Palearctic. However, the genetic distances between the populations from different zoogeographical zones (0.313) did not exceed the level characteristic of the among-population differences, which do not disturb the species genetic integrity.
  Russian Journal of Genetics 12/2008; 45(1):54-62. · 0.43 Impact Factor
• Article: Geographic variability of the polytene chromosome banding sequence of non-biting midge Chironomus pseudothummi Str. (Diptera, Chironomidae)

  I. I. Kiknadze, A. D. Broshkov, A. G. Istomina, L. I. Gunderina, H. Vallenduuk
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  ABSTRACT: The karyotypes and chromosomal polymorphism of Chironomus pseudothummi were investigated in different parts of its areal. It was established that chromosomal variability in the natural populations of this species was represented mainly by the inversion polymorphism of arm G. Only rare and unique inversions were found to be heterozygous in arms C, D, and E. In total, 14 banding sequences of polytene chromosomes form the banding sequence pool of C. pseudothummi. Geographic differences in the distribution of chromosomal banding sequences throughout the areal were established. The presence of banding sequences pstG1 and pstG2 is characteristic of European populations. The banding sequence pstG1 disappeared completely with a simultaneous increase in the frequency of pstG2 and with the appearance of a new inversion banding sequence pstG3 in Siberian populations. Differences in the set of rare and unique inversions in arms C, D, and E between the West-European and West-Siberian populations have been revealed.
  Cell and Tissue Biology 07/2008; 2(4):417-427.
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  Article: Mystery of Chironomus dorsalis Meigen karyotype (Diptera: Chironomidae)

  I I Kiknadze, V V Golygina, A D Broshkov, L I Gunderina, A G Istomina
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  ABSTRACT: The study of the karyotype structure of Chironomus dorsalis Meigen, 1818 from the "pseudothummi" cytocomplex was performed. Arms C and D were mapped for the first time according to Keyl-Dévai system (Keyl, 1962; Dévai et al., 1989) in addition to arms A, E, and F, mapped by Keyl (1962). The structure of the centromeric regions of Ch. dorsalis has been described in more detail and compared with the refer-ence species, Ch. piger Strenzke, 1959, and the other species of the "thummi" cyto-complex. It was discovered that Ch. dorsalis karyotype differs clearly from other Chi-ronomus species by the banding pattern of centromeric regions on the chromosomes AE and BF. Two thick C-positive bands are located on the centromeric region of each of these chromosomes. The hypothesis was suggested that chromosomes AE and BF of Ch. dorsalis are dicentric with one active centromere. The data obtained also sup-port previously postulated hypothesis that the "pseudothummi" cytocomplex should be considered as more primitive and ancestral to other cytocomplexes of the genus Chironomus Meigen, 1803. Natural populations of Ch. dorsalis from various regions of Russia were studied for the first time as well the chromosomal polymorphism in some of these populations was quantitatively estimated that allowed us to assess the size of banding sequence pool for this species, which consists of 8 banding sequences.
  01/2008; 2:21-35.
• Chapter: Chromosomes and Continents

  I. I. Kiknadze, L. I. Gunderina, M. G. Butler, W. F. Wuelker, J. Martin
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  ABSTRACT: The high level of inversion polymorphism and, correspondingly, the abundance of inversion banding sequences (BSs) of polytene chromosomes in the banding sequence pool of Chironomus species permit scientists to reconstruct the cytogenetic evolution of the genus and to evaluate the role of structural rearrangements in the genome during population divergence and speciation. We performed a quantitative assessment of the important role of inversion polymorphism in the differentiation of natural populations and demonstrated the adaptive significance of different gene orders in populations of species occurring in different regions. For the first time, it has been shown that the BS pools of populations of the same species on different continents differed much in the sets and frequencies of gene inversion orders. BS pools of populations on each continent were found to contain continent-specific BSs in addition to sequences occurring on several continents. This intraspecies diversity of the linear organization of the genome is one of the major factors maintaining the evolutionary stability of species in dramatically different environments. In addition to endemic species-specific sequences, the BS pool of the genus Chironomus contains sequences common for different species, cytocomplexes, and continents. These sequences, termed basic sequences, are very important for reconstruction of genome divergence in the course of evolution. It is suggested that they are close to the initial primitive sequences existing on ancient supercontinents, whereas continent-specific BSs were formed after continent separation. Comparison of all currently known BSs in the sequence pool of the genus Chironomus showed that the genomes of the most distant species differed by more than 90 inversion breaks, causing changes of their linear structure. In such cases, conserved genome regions span about 10 bands.
  12/2007: pages 349-369;
• Article: Cytogenetic differentiation of natural populations of Chironomus obtusidens (Diptera, Chironomidae)

  I. I. Kiknadze, A. G. Istomina, L. I. Gunderina
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  ABSTRACT: The cytogenetic structure of Siberian and European populations of Chironomus obtusidens Goetgh. was studied. The clear differentiation of these populations was revealed using quantitative analysis of sets and frequencies of inversion banding sequences. The Siberian populations were found to have six new inversion sequences, and the alternative obtG2 sequence dominated instead of the main obtG1 sequence typical of European populations. In general, chromosomal polymorphism was 3–4 times higher in the Siberian populations. Therefore, the cytogenetic distances (Dcg) between the Siberian and European populations were higher (Dcg 0.059–0.097) than Dcg between the populations in each of the geographic zones investigated (Dcg 0–0.015). The NJ phylogenetic tree constructed for the chromosomal evolution of the genus Chironomus demonstrates that Chironomus obtusidens is the most divergent member among Palaearctic species of the obtusidens group. In addition, it is closer related to the Nearctic Ch. decorus group.
  Entomological Review 09/2007; 87(6):658-669.
• Article: [Polymorphism and differentiation of multilocus DNA markers in natural populations of Drosophila melanogaster].

  O V Vaulin, L I Gunderina, I K Zakharov
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  ABSTRACT: Using multilocus (RAPD) markers, variation and divergence of genomic DNA was examined in two Drosophila melanogaster populations from Russia and three populations from Ukraine. The populations were found to exhibit high polymorphism at RAPD markers. Estimation of genetic distances between the populations showed low differentiation of geographically distant populations of D. melanogaster. Significant gene flow between the D. melanogaster populations was found, which depended on the geographical distance between them.
  Genetika 02/2007; 43(1):61-9. · 0.44 Impact Factor
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  Article: Cytogenetic divergence of genomes in Chironomus plumosus group (Diptera: Chironomidae)

  V V Golygina, I I Kiknadze, A G Istomina, L I Gunderina, L A Miroshnichenko, V D Gusev
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  ABSTRACT: The phylogenetic relationships and karyotype differentiation of 14 sibling species of Chironomus plumosus group were studied using comparative analysis of polytene chromosome banding sequences. Revision of banding sequence pools has been made for all species. Main and alternative banding sequences of 6 chromosomal arms (A, B, C, D, E, and F) of each species were used for the analysis. In total 133 banding sequences were analysed. Phylogenetic trees for each arm were constructed on the basis of break point numbers obtained from the pairwise comparison of banding sequences in the arm. Analysis of divergence of banding sequences in individual chromosomal arms has shown that different arms make an uneven contribution to differentiation of the Ch. plumosus group. Considering the level of divergence and the input of each chromosomal arm into karyotype differentiation of the Ch. plumosus group, the arms could be arranged as: E < F < A ≤ B < D (≤ G) ≤ C. To analyse the phylogenetic relationships of species of Ch. plumosus group integral phylogenetic NJ trees were constructed on the basis of combined data on separate chromosomal arms. It was shown that Chironomus sp. K, Ch. agilis, and Ch. sp. prope agilis are the most divergent species in the Ch. plumosus group. Among the other species Ch. entis, Ch. muratensis, Ch. nudiventris, and Chironomus sp. J also show high level of divergence, as they form separate subcluster. To evaluate how the number of arms used for tree construction influences its topology, 3-arm (A, E, F), 5-arm (A, C, D, E, F) and 6-arm (A, B, C, D, E, F) trees were constructed. The study shows that the combination of data on 5 out of 7 chromosomal arms (about 70% of the genome) provides rather reliable results.
  01/2007; 1:17-32.
• Article: [Variation and divergence of multilocus genome markers in the species of the genus Chironomus (Diptera, Chironomidae)].

  L I Gunderina, I I Kiknadze, A G Istomina, V V Golygina
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  ABSTRACT: Variation and divergence patterns of the multilocus genome markers in twelve Chironomus species belonging to the plumosus and piger sibling-species groups were examined by use of polymerase chain reaction with random primers (RAPD method). The chironomid species showed high levels of the RAPD markers polymorphism. The genetic distances (GD) were determined between the species within the group of closely related species, as well as between the species from different groups. The estimates obtained characterized the divergence levels between the sibling species (GD = 0.739) and morphologically distinct species (GD = 0.935). Comparison of the variation and divergence levels of the RAPD markers with those for the other genome markers, namely, the enzyme-coding genes and chromosomes (gene linkage groups) have demonstrated different divergence rates for different genome components during speciation of Chironimids.
  Genetika 01/2006; 41(12):1634-43. · 0.44 Impact Factor
• Article: [Divergence patterns of banding sequences in different polytene chromosome arms reflect relatively independent evolution of different genome components].

  L I Gunderina, I I Kinadze, A G Istomina, V D Gusev, L A Miroshnichenko
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  ABSTRACT: Divergence patterns of the banding sequences from the chromosomal arms A, C, D, E, and F were compared in 63 species of the genus Chironomus. Evaluation of the number of breakpoints between the pairs of inverted banding sequences and the analysis of the lengths of the conserved segments in the chromosomal arms in the chironomid species examined showed that different arms evolved relatively independently and at different rates. No direct correlation between the arm length and the breakpoints number was observed. The length of the conservative segment was not fixed, but was arm-specific. Robustness and fidelity of the estimates of phylogenetic relationships between the species examined increased with the arm number, i.e., with the genome proportion included in the analysis.
  Genetika 05/2005; 41(4):549-58. · 0.44 Impact Factor
• Article: Divergence patterns of banding sequences in different polytene chromosome arms reflect relatively independent evolution of different genome components

  L. I. Gunderina, I. I. Kiknadze, A. G. Istomina, V. D. Gusev, L. A. Miroshnichenko
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  ABSTRACT: Divergence patterns of the banding sequences of the chromosomal arms A, C, D, E, and F were compared in 63 species of the genus Chironomus. Evaluation of the number of breakpoints between the pairs of inverted banding sequences and the analysis of the lengths of the conserved segments in the chromosomal arms in the chironomid species examined showed that different arms evolved relatively independently and at different rates. No direct correlation between the arm length and the breakpoints number was observed. The length of the conservative segment was not fixed, but was arm-specific. Robustness and reliability of the estimates of phylogenetic relationships between the species examined increased with the arm number, i.e., with the genome proportion included in the analysis.
  Russian Journal of Genetics 03/2005; 41(4):436-444. · 0.43 Impact Factor
• Article: [Divergence of the polytene chromosome banding sequences as a reflection of evolutionary rearrangements of the genome linear structure].

  L I Gunderina, I I Kiknadze, A G Istomina, V D Gusev, L A Miroshnichenko
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  ABSTRACT: Banding sequences of five chromosomal arms (A, C, D, E, and F), accounting for about 70% of the total genome size in 63 Chironomus species, were used as phylogenetic markers to analyze divergence patterns of the linear genome structure during the evolution. The number of chromosomal breakpoints between the pairs of banding sequences compared served as a measure of divergence. It was demonstrated that the greater the divergence between the species compared, the higher the number of chromosomal breakpoints and the smaller the size of the conserved chromosomal regions. A banding sequences comparison in sibling species demonstrated a lower number of chromosomal breakpoints; the breakpoint number was maximum in a comparison of the banding sequences in the subgenera Chironomus and Camptochironomus. The use of the number of chromosomal breakpoints as a divergence measure provided establishment of phylogenetic relationships between 63 Chironomus species and discrimination of sibling species groups and cytocomplexes on a phylogenetic tree.
  Genetika 03/2005; 41(2):187-95. · 0.44 Impact Factor
• Article: [Polymorphism and divergence of multilocus DNA markers in sibling species Chironomus riparius Meigen and Chironomus piger Strenzke (Diptera, Chironomidae)].

  L I Gunderina, E A Salina
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  ABSTRACT: Intra- and interspecific variation and divergence of multilocus markers for genomic DNA of the sibling species from the thimmi group, Chironomus riparius and C. piger, were studied by PCR with arbitrary primers (RAPD). A high level of RAPD polymorphism was determined in both laboratory and natural populations of these species. The genetic distances were estimated between the C. riparius populations and between the sibling species C. riparius and C. piger. The genetic distance between C. riparius and C. piger was 4 to 5 times higher than that between the C. riparius populations. A comparison of the variation and divergence for the RAPD markers with those for other genomic markers--enzyme-coding genes and chromosomes (linked gene groups)--showed that different components of the genome differed in their contribution to the genome divergence.
  Genetika 09/2003; 39(8):1059-65. · 0.44 Impact Factor
• Article: [DNA polymorphism of Drosophila genes and factors determining it].

  L I Gunderina
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  ABSTRACT: The data on variation of DNA sequences in genes of Drosophilidae are reviewed. Intraspecific polymorphism and interspecific divergence of DNA nucleotide sequences are shown to be characteristic of most genes. The level of intraspecific polymorphism and interspecific DNA divergence and the degree of correlation between them depend on the mode, intensity, and direction of natural selection, as well as on the evolutionary history of the genes and species. The evolutionary role of synonymous and nonsynonymous nucleotide substitutions in DNA is analyzed. Genes responsible for the reproduction and genes controlling other functions are compared in respect to their variation.
  Genetika 08/2003; 39(7):888-99. · 0.44 Impact Factor
• Article: [Divergence of karyofunds in sibling species of the plumosus group (Diptera: Chironomidae)].

  L I Gunderina, I I Kiknadze
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  ABSTRACT: Cytogenetic differentiation of eight sibling species of the plumosus group was examined. The karyofunds of these sibling species were shown to diverge incompletely. In each species karyofund, the banding sequences homologous to those of the remaining species of this group were revealed. The number of banding sequences that displayed interspecific homology varied from 3 to 13 per species karyofund. In a species karyotype, the homologous sequences were localized to chromosome arms 1-6. Both similar and contrasting frequencies of homologous banding sequences were observed in karyofunds of different sibling species. The average cytogenetic distance between sibling species of the plumosus group was 2.618 +/- 0.400. The presence of species-specific banding sequences, the absence of homologous banding sequences in some chromosome arms of the karyotype, and different frequencies of the homologous banding sequences determined the cytogenetic divergence of the sibling species.
  Genetika 04/2000; 36(3):339-47. · 0.44 Impact Factor
• Article: [Possibilities of synchronizing the growth of Chironomus thummi Kieff. larvae].

  L I Gunderina, I I Kiknadze
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  ABSTRACT: The conditions for uniform synchronous development of groups of the IV instar larvae of Ch. thummi and the possibility of using the morphology of imaginal discs as a criterion of temporal uniformity (synchrony) of larval development were investigated. The degree of synchrony of larval development was shown to depend strongly on the population density. Its increase led to desynchronization and decrease in the rate of larval development. The size and morphology of imaginal discs used as criteria of developmental phases of the IV instar larvae varied at the "red head" phase. the size of imaginal discs depended on the rate of larval development and population density. No distinct correlation was found between the sex of larvae and the size of imaginal discs in them. A conclusion is drawn that the size of imaginal discs cannot serve as a criterion of temporal uniformity of larval development.
  Ontogenez 13(2):162-8.