Haihan Nan

Chinese Academy of Sciences, Peping, Beijing, China

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Publications (5)8.96 Total impact

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    ABSTRACT: The mangrove plant Pongamia pinnata (Leguminosae) is well known as a plant pesticide. Previous studies have indicated that the flavonoids are responsible of the biological activities of the plant. A new high-speed counter-current chromatography (HSCCC) method for the separation of three flavonoids, karanjin (1), pinnatin (2), and pongaflavone (3), from P. pinnata was developed in the present study. The lower and intermediate phase (LP and IP) of a new three-phase solvent system, n-hexane-acetonitrile-dichloromethane-water, at a volume ratio of 5:5:1:5, were used as the stationary phases, while the upper phase (UP) was used as the mobile phase, and the volume ratio between the stationary phases in the CCC column could be tuned by varying the initial pumped volume ratio of the stationary phases. The CCC columns containing all three phases of the solvent system were considered combination columns. According to the theories of combination column, it is possible to optimize the retention time of the target compounds by varying the volume ratio of the stationary phases in the HSCCC combination columns, as well as the suitable volume ratios of the stationary phases for the separation of the target compounds were predicted from the partition coefficients of the compounds in the three-phase solvent system. Then, three HSCCC separations using the combination columns with initial pumped LP:IP volume ratios of 1:0, 0.9:0.1, and 0.7:0.3 were performed separately based on the prediction. Three target compounds were prepared with high purity when the initial pumped volume ratio of the stationary phases was 0.9:0.1. The baseline separation of compounds 2 and 3 was achieved on the combination column with an initial pumped volume ratio of 0.7:0.3. Furthermore, the three experiments clearly demonstrated that the retentions and resolutions of the target compounds increased with an increasing volume ratio of IP, which is consistent with the prediction for the retention times for the solutes on combination columns. The method proposed here reduces the need for solvent selection compared with the conventional method and may have broad potential applicability in the preparation of natural products.
    Journal of Chromatography A 09/2013; · 4.61 Impact Factor
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    Hao Yin, Jun Wu, Haihan Nan, Si Zhang
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    ABSTRACT: From the stem bark of Pongamia pinnata, two new prenylated flavones (1, 2) were isolated, along with seven known compounds (3–9). Compounds 3 and 4 are isolated for the first time from this plant. The structures of the new compounds were elucidated on the basis of spectroscopic data.
    Pharmazie 02/2006; 61(1):76-8. · 0.96 Impact Factor
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    ABSTRACT: A new prenylated flavon-4-ol with a modified ring A, which we have named pongaflavanol (1), was isolated from the stem bark of Pongamia pinnata along with the known compound tunicatachalcone (2). The structure of compound 1 was elucidated on the basis of spectroscopic data.
    Molecules 02/2006; 11(10):786-91. · 2.43 Impact Factor
  • Hao Yin, Si Zhang, Jun Wu, Haihan Nan
    Journal of The Brazilian Chemical Society - JBCS. 01/2006; 17(7).
  • Source
    Haihan Nan, Jun Wu, Si Zhang
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    ABSTRACT: A new phenylethanoid glycoside, 2-(3-methoxy-4-hydroxylphenyl) ethyl-O-2",3"-diacetyl-alpha-L-rhamnopyranosyl-(1-->3)-4-O-(E)-feruloyl-beta-D-glucopyranoside, was isolated from the aerial parts of Clerodendrum inerme (L.) Gaertn, together with monomelittoside, melittoside, inerminoside A1, verbascoside, isoverbascoside, campneoside I. Their structures were determined by spectroscopic methods.
    Pharmazie 11/2005; 60(10):798-9. · 0.96 Impact Factor

Publication Stats

17 Citations
8.96 Total Impact Points

Institutions

  • 2006
    • Chinese Academy of Sciences
      • Guangdong Key Laboratory of Marine Materia
      Peping, Beijing, China
  • 2005
    • Northeast Institute of Geography and Agroecology
      • Institute of Oceanology
      Beijing, Beijing Shi, China