H Hrselová

Publications (10)10.68 Total impact

• Source

  Available from: cssm.info

  Article: Humic acid-like material from sewage sludge stimulates culture growth of ectomycorrhizal fungi in vitro.

  H Hrselová, L Soukupová, M Gryndler
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  ABSTRACT: Significant effects of humic acid-like material (HALM) extracted from sewage sludge on dry matter production of cultures of ectomycorrhizal basidiomycetes were found in vitro. Mycelial growth of the majority of isolates tended to increase in the presence of the HALM and this effect was significant for 6 isolates. Strongest stimulation was observed in the case of Amanita muscaria, Leccinum aurantiacum and Lactarius deterrimus. The results suggest that the HALM can be used as an additive to media for cultivation of ectomycorrhizal basidiomycetes.
  Folia Microbiologica 02/2007; 52(6):627-30. · 0.68 Impact Factor
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  Available from: Petr Baldrian

  Article: Influence of mineral and organic fertilization on soil fungi, enzyme activities and humic substances in a long-term field experiment.

  V Rezácová, P Baldrian, H Hrselová, J Larsen, M Gryndler
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  ABSTRACT: Changes in microfungal communities, fungal activities and humic substances (HS) in agricultural soils kept under different fertilization regimes were observed and their causal relationships were investigated in a long-term field experiment. Fertilization did not change the abundance of HS-utilizing microfungi and, except for organic amendment alone, total culturable microfungi were also unaffected by this factor. Organic fertilization increased activities of manganese peroxidase (MnP) and proteinase, but decreased endo-1,4-beta-glucanase activity compared to the corresponding control without organic fertilization. In soils treated with mineral fertilizers, the activities of MnP, endo-1,4-beta-glucanase and proteinase were higher than in control without any mineral treatment. Both the aromaticity of fulvic acid and the molar mass of humic acid was lower in soil with organic fertilization, which may be a result of oxidative degradation mediated by higher MnP activity observed in treatments with organic fertilization.
  Folia Microbiologica 02/2007; 52(4):415-21. · 0.68 Impact Factor
• Article: Organic and mineral fertilization, respectively, increase and decrease the development of external mycelium of arbuscular mycorrhizal fungi in a long-term field experiment.

  M Gryndler, J Larsen, H Hrselová, V Rezácová, H Gryndlerová, J Kubát
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  ABSTRACT: Effects of long-term mineral fertilization and manuring on the biomass of arbuscular mycorrhizal fungi (AMF) were studied in a field experiment. Mineral fertilization reduced the growth of AMF, as estimated using both measurements of hyphal length and the signature fatty acid 16:1omega5, whereas manuring alone increased the growth of AMF. The results of AMF root colonization followed the same pattern as AMF hyphal length in soil samples, but not AMF spore densities, which increased with increasing mineral and organic fertilization. AMF spore counts and concentration of 16:1omega5 in soil did not correlate positively, suggesting that a significant portion of spores found in soil samples was dead. AMF hyphal length was not correlated with whole cell fatty acid (WCFA) 18:2omega6,9 levels, a biomarker of saprotrophic fungi, indicating that visual measurements of the AMF mycelium were not distorted by erroneous involvement of hyphae of saprotrophs. Our observations indicate that the measurement of WCFAs in soil is a useful research tool for providing information in the characterization of soil microflora.
  Mycorrhiza 06/2006; 16(3):159-66. · 2.63 Impact Factor
• Article: Hyphal growth and mycorrhiza formation by the arbuscular mycorrhizal fungus Glomus claroideum BEG 23 is stimulated by humic substances.

  M Gryndler, H Hrselová, R Sudová, H Gryndlerová, V Rezácová, V Merhautová
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  ABSTRACT: Effects of humic substances (humic acid or fulvic soil extract) or saprophytic microorganisms (Paecilomyces lilacinus and an unidentified actinomycete) on growth of mycelium and mycorrhiza formation by Glomus claroideum BEG23 were studied in a hydroponic system. Humic substances stimulated root colonization and production of extraradical mycelium by the mycorrhizal fungus. Both humic and fulvic acids tended to decrease populations of culturable bacteria and fungi in the cultivation system, indicating a moderately antibiotic activity. The addition of saprophytic microorganisms able to use humic substances to the cultivation system further stimulated the development of the mycorrhizal fungus. However, stimulation of G. claroideum was also observed when the saprophytic microorganisms were heat-killed, suggesting that their effect was not linked to a specific action on humic substances. The results indicate that humic substances may represent a stimulatory component of the soil environment with respect to arbuscular mycorrhizal fungi.
  Mycorrhiza 12/2005; 15(7):483-8. · 2.63 Impact Factor
• Source

  Available from: cssm.info

  Article: Response of saprotrophic microfungi degrading the fulvic fraction of soil organic matter to different N fertilization intensities, different plant species cover and elevated atmospheric CO2 concentration.

  V Strnadová, H Hrselová, M Kolarík, M Gryndler
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  ABSTRACT: The response of the cenosis composition of soil saprotrophic microfungi able to utilize the fulvic fraction of soil organic matter to increased concentration of atmospheric carbon dioxide, plant species cover quality and different levels of nitrogen fertilization was determined under field conditions in a free-air carbon dioxide enrichment experiment. Twenty-nine species of microfungi were isolated from the tested soil. The effects of CO2 enrichment and plant species cover were not significant. Nitrogen fertilization was identified as the only significant factor inducing changes in the abundance of soil microorganisms. This was reflected in a relatively low value of quantitative Sørensen similarity index on comparing fertilized and unfertilized treatments and in 2-way ANOVA of total CFU counts. Some differences were observed in species diversity between the two variants of all treatments. No association between microfungi and the factors under study was found by using the Monte Carlo Permutation test in redundancy analysis.
  Folia Microbiologica 02/2004; 49(5):563-8. · 0.68 Impact Factor
• Article: Long-term fertilization affects the abundance of saprotrophic microfungi degrading resistant forms of soil organic matter.

  M Gryndler, H Hrselová, J Klír, J Kubát, J Votruba
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  ABSTRACT: The effect of mineral and organic fertilization on the occurrence of soil microorganisms was determined in a field experiment. The colony-forming unit counts of saprotrophic microfungi, when estimated on a silicate gel medium containing fulvic acid as a sole carbon source, increased significantly with increasing doses of mineral and organic fertilization. Partial correlation analysis indicated that, unlike bacteria and actinomycetes, microfungi utilizing fulvic acid were significantly associated with soil organic carbon. No significant effects on bacteria and microfungi counted on common microbiological media were observed but counts of actinomycetes increased in a manured soil extensively fertilized by a mineral fertilizer. Fulvic acid utilizing microfungi, which are associated with areas rich in organics, play possibly the main role in mineralization of resistant forms of soil organic matter.
  Folia Microbiologica 02/2003; 48(1):76-82. · 0.68 Impact Factor
• Article: The effect of fulvic acids on the toxicity of lead and manganese to arbuscular mycorrhizal fungus Glomus intraradices.

  R Malcová, M Gryndler, H Hrselová, M Vosátka
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  ABSTRACT: The effect of fulvic acids (FA) on arbuscular mycorrhizal (AM) fungi and on the toxicity of lead and manganese toward these symbionts were demonstrated in vitro. Incubation of root segments colonized with the AM fungus Glomus intraradices in undiluted fraction of FA (813 mg/L carbon) decreased an outgrowth of intraradical hyphae. Diluted FA solutions (< 271 mg/L C) did not influence the proportion of root segments bearing proliferating hyphae; solution containing 27.1 mg/L C even increased the proliferation. A decrease of heavy metal toxicity toward the fungus was observed when FA (81.3 mg/L C) were added to the solutions containing higher concentrations (< or = 100 mumol/L) of Mn and Pb; the positive effect of FA was not significant at higher concentrations of metals (0.5 mmol/L). A short-term cultivation of six different saprophytic microorganisms--three actinomycetes and three filamentous fungi--on the FA media (81.3 mg/L C) did not result in a modification of hyphal proliferation from the root segments subsequently incubated in these media.
  Folia Microbiologica 01/2002; 47(5):521-6. · 0.68 Impact Factor
• Article: Organic fertilization changes the response of mycelium of arbuscular mycorrhizal fungi and their sporulation to mineral NPK supply.

  M Gryndler, H Hrselová, M Vosátka, J Votruba, J Klír
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  ABSTRACT: The synergetic effect of organic (cow manure) and mineral fertilization on the development arbuscular mycorrhizal (AM) fungi was demonstrated. The length of AM mycelium and sporulation were used as sensitive markers of the physiological state of soil AM fungal population. In manured treatments, both parameters increased in proportion with increasing mineral fertilization. In unmanured soil, the opposite trend was observed for the length of AM hyphae, which decreased with increasing mineral fertilization. Correlation analysis showed the dependence of length of AM hyphae and sporulation on soil available phosphorus. The correlation was negative in soil with no mineral fertilization and positive in soil supplied with luxury doses of mineral fertilizer.
  Folia Microbiologica 02/2001; 46(6):540-2. · 0.68 Impact Factor
• Article: Effect of soil bacteria on hyphal growth of the arbuscular mycorrhizal fungus Glomus claroideum.

  M Gryndler, H Hrselová, D Stríteská
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  ABSTRACT: The effect of 46 bacterial strains isolated from tilled and non-tilled soils collected at 3 localities on the growth of intraradical hyphae of the arbuscular mycorrhizal (AM) fungus Glomus claroideum was demonstrated. A larger number of stimulatory bacterial isolates was obtained from tilled soils, but the bacteria showing the strongest stimulation of hyphal growth were isolated from a soil that had not been cultivated. Isolates obtained from hyphae of AM fungi showed no substantial stimulatory effects, but produced more uniform effects on hyphal growth than the isolates of bacteria obtained from soil. Bacterial cenoses present in 3 different soils differ significantly in their effects on AM fungi.
  Folia Microbiologica 02/2000; 45(6):545-51. · 0.68 Impact Factor
• Article: Effect of spermine on proliferation of hyphae of Glomus fistulosum, an arbuscular mycorrhizal fungus, in maize roots.

  H Hrselová, M Gryndler
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  ABSTRACT: Effects of two oligoamines, putrescine and spermine, on proliferation of intraradical hyphae in surface disinfected root segments were studied under axenic conditions in vitro. No significant effects of putrescine were observed. Spermine significantly stimulated hyphal growth at a concentration of about 1.5 mumol/L. High concentration (> 150 mumol/L) caused a strong inhibition of hyphal growth and of the percentage of root segments bearing proliferating hyphae. DL-alpha-difluoromethylornithine, a metabolic inhibitor of polyamine synthesis, caused a significant inhibition of proliferation of the hyphae only in the presence of 2 mumol/L spermine.
  Folia Microbiologica 02/2000; 45(2):167-71. · 0.68 Impact Factor