Grégoire Jacques Noël Cozon

Publications (2)4.52 Total impact

• Article: Abnormal cellular reactivity to microbial antigens in patients with uveitis.

  Grégoire Jacques Noël Cozon, Florentin Martial Mbitikon-Kobo, Fathi Fatoohi, Myriam Spire, Jean-Daniel Grange, Laurent Kodjikian
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  ABSTRACT: The purpose of the present study was to evaluate the cellular response to microbial antigens in patients with idiopathic uveitis. Blood lymphocytes from 31 patients with uveitis and 24 healthy controls were cultivated with microbial antigens and analyzed by flow cytometry after staining with monoclonal antibodies against CD3, CD4, and activation markers CD69 and CD25. Although no difference was noted in circulating lymphocytes, the activation of T cells, detected with CD69, was higher in 24-hour blood culture from uveitis patients with Candida albicans antigen (Ca-Ag) than from controls, especially in posterior uveitis and panuveitis. Moreover, late response, detected with CD25, to different microbial antigens was higher in patient with uveitis. Such results suggest the role of Ca-Ag and microbial antigens in the pathogenic mechanisms of idiopathic uveitis.
  Investigative Ophthalmology &amp Visual Science 07/2008; 49(6):2526-30. · 3.60 Impact Factor
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  Article: Systemic T cell response to Toxoplasma gondii antigen in patients with ocular toxoplasmosis.

  Fatih Fatoohi, Grégoire Jacques Noël Cozon, Martine Wallon, Laurent Kodjikian, François Peyron
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  ABSTRACT: Analysis of systemic cellular response to Toxoplasma antigen in patients with ocular toxoplasmosis. Activated (CD25(+)) T cells were detected by flow cytometry after a 7-day culture of whole blood from patients with ocular (n = 16) or asymptomatic (n = 14) toxoplasmosis, and controls (n = 10), in the presence of soluble Toxoplasma antigen (ST-Ag). Interferon (IFN)-gamma, interleukin (IL) 4, and IL-10 were measured in culture supernatants by enzyme-linked immunosorbent assay. Higher percentages of CD25(+) T cells were detected in ST-Ag-activated cultures from Toxoplasma-infected patients, with or without ocular lesions (37.0 +/- 19.1% or 41.1 +/- 19.3%, respectively) than from controls (3.2 +/- 1.2%) (P < 0.0001). Differences were not statistically significant between asymptomatic and ocular toxoplasmosis (P > 0.4) or among congenital, acquired, and undetermined ocular toxoplasmosis (P > 0.2). Higher levels of IFN-gamma were detected in ST-Ag-stimulated blood cultures from infected patients than in those from controls (P < 0.0001), with no difference between patients with asymptomatic or ocular toxoplasmosis (P > 0.05). IL-10 was detected only in activated culture supernatants from three patients with ocular toxoplasmosis and two patients with asymptomatic toxoplasmosis. IL-4 was never produced in ST-Ag-activated cultures. Systemic cellular response to ST-Ag does not differ between the patients with ocular and asymptomatic toxoplasmosis with regard to activation markers and type 1 cytokine production.
  Japanese Journal of Ophthalmology 50(2):103-10. · 0.92 Impact Factor