Fábio Rogério Rosado

Universidade Federal do Paraná, Curitiba, Estado do Parana, Brazil

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Publications (8)10.59 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: The two main water-soluble extracellular polysaccharides produced by the basidiomycete fungus Pleurotus ostreatoroseus Sing were isolated and purified. They were characterized using 13C, 1H, and 1H,13C HMQC NMR spectroscopy, methylation analysis, and Smith degradation. One was a mannan having a main chain of (1→6)-linked -d-mannopyranosyl residues, almost all of which were branched at O-2 with side chains of different lengths, containing 2-O- and 3-O-linked mannopyranosyl units. The other was a partially 3-O-methylated (1→4)-linked -d-galactopyranan, a structure that has not been previously described.
    FEMS Microbiology Letters 01/2006; 212(2):261 - 265. · 2.05 Impact Factor
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    ABSTRACT: The glucans of basidiomycetes are an important class of polysaccharides with potential biological activities. In this work, the β-glucans were isolated from the fruiting bodies of edible mushrooms, Pleurotus eryngii and Pleurotus ostreatoroseus, via extraction with hot water, and then fractionation by freeze-thawing. The insoluble glucans gave similar 13C NMR spectra, monosaccharide composition and methylation analyses, and P. eryngii was selected for further controlled Smith degradation, and DEPT and 1H (obs.), 13C HMQC spectroscopy. It was a branched β-glucan, with a main chain of (1 → 3)-linked-Glcp residues, substituted at O-6 by single-unit β-Glcp side-chains, on average to every third residue of the backbone, as in scleroglucan.
    Carbohydrate Polymers. 01/2006;
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    ABSTRACT: The two main polysaccharides from the basidiomycetous fungus Laetiporus sulphureus were isolated, purified and characterized. The structural assignments were carried out using (13)C, (1)H, and (1)H,(13) HSQC nuclear magnetic resonance spectroscopy, methylation analysis, and Smith degradation. One was a linear beta-glucan having a (1-->3)-linked main chain, namely laminaran. The other was a fucomannogalactan, which consisted of a main chain of (1-->6)-linked alpha-D-galactopyranosyl residues, a part of them being substituted at O-2 by 3-O-D-mannopyranosyl-L-fucopyranosyl, alpha-D-mannopyranosyl and in a minor proportion, alpha-L-fucopyranosyl groups. This heteropolysaccharide is related to those of other Basidiomycetes heterogalactans, although it differs distinctly in its side-chain structures. Whereas part of the single-unit L-fucopyranosyl and/or 3-O-alpha-mannopyranosyl-L-fucopyranosyl residues are present as side chains of the other heterogalactans, additional alpha-D-mannopyranosyl units are present in our fucomannogalactan of L. sulphureus.
    FEMS Microbiology Letters 01/2004; 230(1):47-52. · 2.05 Impact Factor
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    ABSTRACT: The partially 3-O-methylated mannogalactans were isolated from the fruiting bodies of edible basidiomycetes Pleurotus ostreatus 'florida' Berk. and Pleurotus ostreatoroseus Sing. They were obtained via successive aqueous extraction, freeze thawing, and precipitation with Fehling solution and then investigated using (13)C- and (1)H-nuclear magnetic resonance spectroscopy (including COSY, TOCSY and HMQC techniques), methylation analysis and Smith degradation. The main chain consisted of (1-->6)-linked alpha-D-galactopyranosyl residues containing 3-O-Me-alpha-D-galactopyranoses, a part of these units being substituted in the position O-2 with beta-D-mannopyranose residues. The heteropolysaccharides found were similar with differences only in the levels of the 3-O-Me-alpha-D-galactopyranoses residues. The presence of partially 3-O-methylated mannogalactan appears to be typical of Pleurotus spp.
    FEMS Microbiology Letters 04/2003; 221(1):119-24. · 2.05 Impact Factor
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    ABSTRACT: The species of the genus Pleurotus produce large amounts of biomass and exopolysaccharide (EPS) in submerged cultures, which may be used for biotechnological purposes. In the present work two Brazilian autochthonous strains of edible Pleurotus (P. ostratoroseus Sing. and P. ostreatus "florida") were used. The fungi grown in liquid Potato Dextrose medium (PD) were used as inocula to cultivate those microorganisms in the POL culture medium. After a 9-day incubation, the optimal growth time for biomass production, P. ostreatus "florida" presented higher biomass production (22.8 g d.w./l) than P. ostreatoroseus (16.8 g d.w./l). After a 7-day incubation, the optimal growth time for EPS production, P. ostreatoroseus produced higher amounts of crude EPS (5.8 g d.w./l) than P. ostreatus "florida" (1.4 g d.w./l). Relative carbohydrate composition for P. ostreatoroseus and P. ostreatus "florida" EPS were: glucose (95.5-87.7), galactose (traces - 4.9), mannose (traces - 3.1), xylose (1.3-2.5), and arabinose (3.2-1.8). Lower ammonium sulfate concentration in the POL culture medium increased the exopolysaccharides production by P. ostreatoroseus.
    Journal of Basic Microbiology 02/2003; 43(3):230-7. · 1.20 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: The two main water-soluble extracellular polysaccharides produced by the basidiomycete fungus Pleurotus ostreatoroseus Sing were isolated and purified. They were characterized using 13C, 1H, and 1H, 13C HMQC NMR spectroscopy, methylation analysis, and Smith degradation. One was a mannan having a main chain of (1-->6)-linked alpha-D-mannopyranosyl residues, almost all of which were branched at O-2 with side chains of different lengths, containing 2-O- and 3-O-linked mannopyranosyl units. The other was a partially 3-O-methylated (1-->4)-linked alpha-D-galactopyranan, a structure that has not been previously described.
    FEMS Microbiology Letters 07/2002; 212(2):261-5. · 2.05 Impact Factor
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    ABSTRACT: Efficiency of solid and liquid inocula and their use for spawn production were compared so that improved cultivation conditions for the edible mushroom Pleurotus ostreatoroseus could be tested. Solid and liquid inocula were prepared respectively with Potato Dextrose Agar (PDA) and Liquid Potato Dextrose (LPD). Wheat grains and cotton residues were used as substrates for spawn preparation. Inoculum types did not affect the development of P. ostreatoroseus, and LPD spawns were cheaper, more homogenous, less contaminated. Decomposition activity of mushroom growth, as a percentage of organic matter loss (OML), was higher in the wheat grain spawn and was not influenced by the inoculum type. Advantages in the use of cotton residue for spawn production were longer storage time, lower contamination and reduced costs. The cotton residue substrate may be also used for the production of mushroom fruiting bodies.
    Journal of Basic Microbiology 02/2002; 42(1):37-44. · 1.20 Impact Factor
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    Damiana Maria, Ferdinandi, Fábio Rogério Rosado
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    ABSTRACT: RESUMO: Os cogumelos comestíveis e as plantas medicinais estão ganhando importância na atualidade pelos seus valores nutritivos e terapêuticos. O fungo Basidiomicete Pleurotus ostreatus é considerado de alta importância, porque, além da produção de exopolissacarídeos e biomassa, possui substâncias terapêuticas ativas. A planta medicinal Ginkgo biloba é oriunda da China e possui constituintes farmacológicos ativos presentes nas folhas, sementes e raízes que vêm sendo usados para a prevenção e tratamento de doenças. Foi avaliada a produção de biomassa de Pleurotus ostreatus em cultura submersa, o fungo foi inoculado em meio de cultura BD líquida em diferentes concentrações do extrato aquoso de Ginkgo biloba. Estes inóculos que contêm o fungo foram transferidos para erlenmyers e submetidos à agitação a 150 rpm por 14 dias. Após determinação de biomassa, houve crescimento micelial e o extrato desta planta pode ser uma alternativa para estimular o crescimento do fungo e aplicá-lo na indústria Nutricêutica e em Processos Biotecnológicos. ABSTRACT: The importance of comestible mushrooms and medicinal herbs is currently on the increase owing to their nutrition and therapeutic properties. The basidiomycete fungus Pleurotus ostreatus is highly important due to its active therapeutic compounds besides its production of exopolysaccharides and biomass. Hailing from China, the medicinal plant Ginkgo biloba has active pharmaceutical properties in leaves, seeds and roots which are used for the prevention and treatment of several diseases. Production of Pleurotus ostreatus products in a submerged culture has been evaluated. Fungus was inoculated in liquid BD medium at different concentrations of Ginkgo biloba aqueous extract. Inocula with fungus were transferred to Erlenmeyer flasks and stirred for 14 days at 150 rpm. Posterior to biomass determination, mycelial growth occurred and the plant extract may be an alternative to stimulate fungus growth. Its application in nutritional and biotechnological productions is highly appreciated.

Publication Stats

80 Citations
106 Downloads
539 Views
10.59 Total Impact Points

Institutions

  • 2004–2006
    • Universidade Federal do Paraná
      • Departamento de Bioquímica
      Curitiba, Estado do Parana, Brazil
  • 2002–2006
    • Universidade Estadual de Maringá
      • Departamento de Bioquímica
      Maringá, Estado do Parana, Brazil
  • 2002–2003
    • Centro Universitário de Maringá
      Maringá, Paraná, Brazil