Elke Stoffels

Publications of Elke Stoffels

• Histological studies of bone formation during pedicle restoration and early antler regeneration in roe deer and fallow deer.

  Authors: Uwe Kierdorf, Elke Stoffels, Dieter Stoffels, Horst Kierdorf, Thomas Szuwart, Günter Clemen

  The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology. 09/2003; 273(2):741-51.

  The purpose of the present study was to examine the process of bone formation in the regenerating cranial appendages of roe deer (Capreolus capreolus) and fallow deer (Dama dama) during the earlyThe purpose of the present study was to examine the process of bone formation in the regenerating cranial appendages of roe deer (Capreolus capreolus) and fallow deer (Dama dama) during the early postcasting period. After the antlers are cast, osteoclastic and osteoblastic activities lead to a smoothing of the pedicle's separation surface, a strengthening of the pedicle bone, and a partial restoration of the distal pedicle portion that was lost along with the cast antler. Initially, bone formation occurs by intramembranous ossification, but early during the regeneration process cartilage is formed at the tips of the cranial appendages, and is subsequently replaced by bone in a process of endochodral ossification. Shortly after the antlers are cast, the cambium layer of the periosteum in the distal pedicle is markedly enlarged, which suggests that the periosteum serves as a cell source for the bone-forming tissue covering the exposed pedicle bone. The histological findings of our study are consistent with the view that the bony component of the regenerating cranial appendages of deer is largely derived from the pedicle periosteum. Based on findings in other bone systems, we speculate that stem cells that can undergo both osteogenic and chondrogenic differentiation are present in the pedicle periosteum. The early onset of chondrogenesis in the regeneration process is regarded as an adaptation to the necessity of producing a huge volume of bone within a short period. This parallels the situation in other cases of chondrogenesis in membrane bones.

• Image-based extracorporeal tissue engineering of individualized bone constructs.

  Authors: Ulrich Meyer, Andre Büchter, Ariane Hohoff, Elke Stoffels, Thomas Szuwart, Christoph Runte, Dieter Dirksen, Hans-Peter Wiesmann

  The International journal of oral & maxillofacial implants. 20(6):882-90.

  PURPOSE: Computer-aided technologies have been recently employed for use in extracorporeal bone tissue engineering strategies. In this pilot animal experimental study, the intention was to testPURPOSE: Computer-aided technologies have been recently employed for use in extracorporeal bone tissue engineering strategies. In this pilot animal experimental study, the intention was to test whether autologous osteoblast-like cells cultured in vitro on individualized scaffolds can be used to support bone regeneration in a clinical environment. MATERIALS AND METHODS: For this purpose, mandibular bone defects were surgically introduced into the mandibles of minipigs and the scaffold of the defect site was modeled by computer-aided design/computer-aided manufacturing technique. Autologous bone cells from porcine calvaria were harvested from minipigs and grown in culture. Cells were seeded on scaffolds generated by rapid prototyping of polylactic acid/polyglycolic acid copolymers. The defects were then reconstructed by implanting the tissue constructs. RESULTS: The intraoperative sites as well as the postoperative computerized tomographic scans demonstrated an accurate fit in the defect sites. The implanted scaffold constructs enriched with osteoblast-like cells were well tolerated and appeared to support bone formation, as revealed by histologic and immunohistochemical analyses. DISCUSSION: These results indicated that in vitro expanded osteoblast-like cells spread on a resorbable individualized scaffold can be capable of promoting the repair of bony defects in vivo. CONCLUSION: These results warrant further attempts to combine computer modeling and tissue engineering for use in bone reconstructive surgery.