Dik J Mevius

Publications (12)23.42 Total impact

• Article: [In Process Citation].

  Joost Hordijk, Jaap A Wagenaar, Dik J Mevius
  Tijdschrift voor diergeneeskunde 03/2013; 138(3):156-9, 161. · 0.19 Impact Factor
• Article: [Identical resistance genes and plasmids in Escherichia coli from Dutch patients, poultry and retail chicken meat].

  Maurine A Leverstein-van Hall, Cindy M Dierikx, James Cohen Stuart, Guido M Voets, Thijs P van den Munckhof, Tamara N Platteel, Ad C Fluit, Nienke van de Sande-Bruinsma, Marc J M Bonten, Dik J Mevius
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  ABSTRACT: To determine the distribution of extended-spectrum beta-lactamase (ESBL) genes and plasmids in Escherichia coli present in broilers and retail chicken meat from the Netherlands as well as in a representative sample of clinical E. coli isolates from patients in the Netherlands. Descriptive. The ESBL-producing E. coli isolates originated from 98 chicken fillets, an ESBL-surveillance study of broilers dating from 2006, and 516 human clinical samples from 31 laboratories collected during a 3-month period in 2009. The distribution of ESBL genes and plasmids in E. coli present in broilers and fresh chicken was determined and the ESBL genes that were found were defined as 'poultry-associated' (PA). In a random survey, representative for the Netherlands, of the clinical E. coli isolates, the proportion of PA ESBL genes and plasmids was quantified. Isolates were analysed using an ESBL-specific microarray, an ESBL gene sequence determination method and two plasmid-typing methods: the so-called PCR-based replicon typing, and plasmid multi-locus sequence typing (pMLST). Six ESBL genes were defined as PA: blaCTXM-1, blaCTXM-2, blaSHV-2, blaSHV-12, blaTEM-20, blaTEM-52; 35% of the human ESBL-producing isolates contained PA ESBL genes and 19% contained PA ESBL genes located on IncI1 plasmids that were genetically indistinguishable from those obtained from broilers. In human isolates with a PA ESBL, the most prevalent (86%) genes were blaCTXM-1 and blaTEM-52, which were also the predominant genes in broilers (78%), and chicken fillets (75%). 94% of the 98 chicken fillets contained ESBL producing E. coli. These findings suggest that ESBL producing E. coli may be transmitted from poultry to humans via the food chain.
  Nederlands tijdschrift voor geneeskunde 01/2011; 155:A3401.
• Source

  Available from: Xander W Huijsdens

  Article: Unexpected sequence types in livestock associated methicillin-resistant Staphylococcus aureus (MRSA): MRSA ST9 and a single locus variant of ST9 in pig farming in China.

  Jaap A Wagenaar, Hua Yue, Jane Pritchard, Marian Broekhuizen-Stins, Xander Huijsdens, Dik J Mevius, Thijs Bosch, Engeline Van Duijkeren
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  ABSTRACT: In October 2008 nine farrow-to-finish pig farms were visited in Shuangliu County in Sichuan Province, China. One farm was empty for one month but not cleaned after depopulation. Dust samples were collected at each farm and analysed for the presence of methicillin-resistant Staphylococcus aureus (MRSA). Dust samples from four farms were also analysed for the presence of methicillin-susceptible S. aureus (MSSA). On 5/9 farms MRSA was isolated and on 2/4 farms MSSA was isolated. On two farms, including the empty farm, no MRSA or MSSA could be detected. All MRSA isolates (n=43) belonged to spa type t899. MSSA isolates belonged to spa type t899 (n=12) and spa type t034 (n=2). From 4/9 farms the MRSA isolates of spa type t899 were assigned to multilocus sequence type (MLST) ST9 whereas on one farm the MRSA spa type t899 isolates belonged to a single locus variant of MLST ST9 (ST1376). MSSA isolates with spa type t899 belonged to MLST ST9 and the MSSA with spa type t034 belonged to MLST ST398. This is the first report on MRSA in pig farms in China and the first time that MRSA ST9 and a single locus variant of ST9 are detected in pig farms. This study shows that livestock associated MRSA is not restricted to clonal lineage ST398 as found in Europe and Northern America in commercial pigs but that other MRSA lineages are able to spread in livestock as well. The study confirms that livestock may act as a reservoir for MRSA.
  Veterinary Microbiology 07/2009; 139(3-4):405-9. · 3.33 Impact Factor
• Source

  Available from: Ingeborg Marianne van Geijlswijk

  Article: [Quantity of veterinary antibiotic use].

  Inge M van Geijlswijk, Dik J Mevius, Linda F Puister-Jansen
  Tijdschrift voor diergeneeskunde 02/2009; 134(2):69-73. · 0.19 Impact Factor
• Source

  Available from: Rene S Hendriksen

  Article: Occurrence of antimicrobial resistance among bacterial pathogens and indicator bacteria in pigs in different European countries from year 2002 - 2004: the ARBAO-II study.

  Rene S Hendriksen, Dik J Mevius, Andreas Schroeter, Christopher Teale, Eric Jouy, Patrick Butaye, Alessia Franco, Andra Utinane, Alice Amado, Miguel Moreno, Christina Greko, Katharina D C Stärk, Christian Berghold, Anna-Liisa Myllyniemi, Andrzej Hoszowski, Marianne Sunde, Frank M Aarestrup
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  ABSTRACT: The project "Antibiotic resistance in bacteria of animal origin - II" (ARBAO-II) was funded by the European Union (FAIR5-QLK2-2002-01146) for the period 2003-05. The aim of this project was to establish a program for the continuous monitoring of antimicrobial susceptibility of pathogenic and indicator bacteria from food animals using validated and harmonised methodologies. In this report the first data on the occurrence of antimicrobial resistance among bacteria causing infections in pigs are reported. Susceptibility data from 17,642 isolates of pathogens and indicator bacteria including Actinobacillus pleuropneumoniae, Streptococcus suis and Escherichia coli isolated from pigs were collected from fifteen European countries in 2002-2004. Data for A. pleuropneumoniae from infected pigs were submitted from five countries. Most of the isolates from Denmark were susceptible to all drugs tested with the exceptions of a low frequency of resistance to tetracycline and trimethoprim - sulphonamide. Data for S. suis were obtained from six countries. In general, a high level of resistance to tetracycline (48.0 - 92.0%) and erythromycin (29.1 - 75.0%) was observed in all countries whereas the level of resistance to ciprofloxacin and penicillin differed between the reporting countries. Isolates from England (and Wales), France and The Netherlands were all susceptible to penicillin. In contrast the proportion of strains resistant to ciprofloxacin ranged from 12.6 to 79.0% (2004) and to penicillin from 8.1 - 13.0% (2004) in Poland and Portugal. Data for E. coli from infected and healthy pigs were obtained from eleven countries. The data reveal a high level of resistance to tetracyclines, streptomycin and ampicillin among infected pigs whereas in healthy pigs the frequency of resistance was lower. Bacterial resistance to some antimicrobials was frequent with different levels of resistance being observed to several antimicrobial agents in different countries. The occurrence of resistance varied distinctly between isolates from healthy and diseased pigs, with the isolates from healthy pigs generally showing a lower level of resistance than those from diseased pigs. The study suggests that the choice of antimicrobials used for the treatment of diseased animals should preferably be based on knowledge of the local pattern of resistance.
  Acta Veterinaria Scandinavica 02/2008; 50:19. · 1.00 Impact Factor
• Source

  Available from: Kees Veldman

  Article: External quality assurance system for antibiotic resistance in bacteria of animal origin in Europe (ARBAO-II), 2003.

  Danilo M A Lo Fo Wong, Rene S Hendriksen, Dik J Mevius, Kees T Veldman, Frank M Aarestrup
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  ABSTRACT: Initiated in 2003 by the European Union, ARBAO-II aims to establish a monitoring of antimicrobial susceptibility among the veterinary laboratories in all European countries based on validated methodologies. This includes an external quality control system for the most important bacterial pathogens. In 2003 two trials were performed. The first on Salmonella and Escherichia coli involved 31 laboratories in 18 countries and the second on Staphylococcus and Streptococcus included 22 laboratories in 15 countries. For the E. coli strains, 92.8% of the results complied with the reference MICs, for Salmonella 93.7%, for Streptococcus 80.4% and for Staphylococcus 93.1%. Most problems were observed when testing florfenicol (79.2% correct), gentamicin (84.2%) and amoxicillin + cl (84.9%) in E. coli; streptomycin in Salmonella (62.5%); gentamicin (56.7%), lincomycin (71.4%), clindamycin (75.4%), TMP + sulfonamides (75.7%) and chloramphenicol (78.5%) in Streptococcus; erythromycin (81.5%) and oxacillin (78.2.5%) in Staphylococcus. A few laboratories caused most deviations. However, there was no correlation between good performance for one bacterial group and good performance for other groups. This study showed that most laboratories are capable of performing correct susceptibility testing for E. coli and Salmonella, even though performance of some laboratories can be improved, and that some problems exist for Staphylococcus and Streptococcus. This clearly shows the need for continuous harmonisation of methodologies within the EU.
  Veterinary Microbiology 07/2006; 115(1-3):128-39. · 3.33 Impact Factor
• Source

  Available from: Chris Van den Eede

  Article: Quantitative susceptibility of Streptococcus suis strains isolated from diseased pigs in seven European countries to antimicrobial agents licensed in veterinary medicine.

  Henk J Wisselink, Kees T Veldman, Chris Van den Eede, Sarah A Salmon, Dik J Mevius
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  ABSTRACT: The susceptibility of Streptococcus suis strains (n=384) isolated from diseased pigs in seven European countries to 10 antimicrobial agents was determined. For that purpose a microbroth dilution method was used according to CLSI recommendations. The following antimicrobial agents were tested: ceftiofur, cefquinome, enrofloxacin, florfenicol, gentamicin, penicillin, spectinomycin, tetracycline, tilmicosin and trimethoprim/sulphamethoxazole. Using breakpoints established by CLSI for veterinary pathogens, all strains were susceptible to ceftiofur, florfenicol, enrofloxacin and penicillin. MIC-90 values of these antibiotics were < or = 0.03, 0.5, 2 and < or = 0.13 microg/mL, respectively. A low degree of resistance was observed for gentamicin (1.3%), spectinomycin (3.6%) and trimethoprim/sulphamethoxazole (6.0%). MIC-90 values of these antibiotics were 8, 16 and 2 microg/mL, respectively. A high level of resistance was observed for tetracycline (75.1%). A MIC-90 value of 64 microg/mL was found for this antibiotic. Serotype-associated differences in MIC-90 values were observed for tetracycline, tilmicosin and trimethoprim/suphamethoxazole.
  Veterinary Microbiology 03/2006; 113(1-2):73-82. · 3.33 Impact Factor
• Article: Integrons in Escherichia coli from food-producing animals in The Netherlands.

  Adrienne T A Box, Dik J Mevius, Pepijn Schellen, Jan Verhoef, Ad C Fluit
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  ABSTRACT: The presence and character of class 1 integrons in multidrug-resistant Escherichia coli from slaughter animals and meat was determined by integrase-specific PCR and conserved segment PCR-restriction fragment length polymorphism (RFLP). At least five different class 1 integron types were found and three types were shared between hospitalized patients, humans in the community, meat, and slaughter animals. Common integron types indicate that antibiotic resistance genes are exchanged via the food chain between different reservoirs of both human and animal origin.
  Microbial Drug Resistance 02/2005; 11(1):53-7. · 2.15 Impact Factor
• Article: Salmonella gene rma (ramA) and multiple-drug-resistant Salmonella enterica serovar typhimurium.

  Tahar van der Straaten, Riny Janssen, Dik J Mevius, Jaap T van Dissel
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  ABSTRACT: MarA and its homologue, RamA, have been implicated in multidrug resistance (MDR). RamA overexpression in Salmonella enterica serovar Typhimurium and Escherichia coli conferred MDR independently of marA. Inactivation of ramA did not affect the antibiotic susceptibilities of wild-type S. enterica serovar Typhimurium or 15 unrelated clinical MDR isolates. Thus, ramA overexpression is not a common MDR mechanism in Salmonella.
  Antimicrobial Agents and Chemotherapy 07/2004; 48(6):2292-4. · 4.84 Impact Factor
• Article: Rapid selection of quinolone resistance in Campylobacter jejuni but not in Escherichia coli in individually housed broilers.

  Michiel van Boven, Kees T Veldman, Mart C M de Jong, Dik J Mevius
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  ABSTRACT: To determine the within-host population dynamics of Campylobacter jejuni and Escherichia coli in chickens during and after treatment with fluoroquinolones. Total and resistant faecal counts were determined from cloacal swabs during and after treatment with enrofloxacin. Chickens were housed individually to avoid confounding as a result of interaction between animals, and to be able to focus solely on the within-host dynamics. To determine the molecular basis of resistance, a number of isolates were checked for mutations in gyrA. Treatment with enrofloxacin at doses routinely prescribed (50 ppm) rapidly reduced the faecal counts of E. coli below the detection limit and did not induce resistance. In C. jejuni, on the other hand, treatment with enrofloxacin quickly selected for high frequencies of fluoroquinolone-resistant strains. In all phenotypically resistant isolates, resistance was traced to mutations in the gyrA gene. (1) A licensed dosage (50 ppm) of enrofloxacin in drinking water of chickens is effective (i.e. markedly reduced faecal counts) and is safe on a short time scale in E. coli (i.e. did not rapidly select for resistance), but is neither safe nor effective in C. jejuni. (2) The rapid emergence of resistance to quinolones in C. jejuni does not necessarily result from horizontal transmission of resistant strains among chickens, but could solely be the result of de novo selection of resistance in individual chickens.
  Journal of Antimicrobial Chemotherapy 11/2003; 52(4):719-23. · 5.07 Impact Factor
• Source

  Available from: Dariusz Wasyl

  Article: Prevalence of antimicrobial resistance among bacterial pathogens

  Rene S Hendriksen, Dik J Mevius, Andreas Schroeter, Christopher Teale, Danièle Meunier, Patrick Butaye, Alessia Franco, Andra Utinane, Alice Amado, Miguel Moreno, Christina Greko, Katharina Stärk, Christian Berghold, Anna-Liisa Myllyniemi, Dariusz Wasyl, Marianne Sunde, Frank M Aarestrup
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  ABSTRACT: Background: The project "Antibiotic resistance in bacteria of animal origin – II" (ARBAO-II) was funded by the European Union (FAIR5-QLK2-2002-01146) for the period 2003–2005, with the aim to establish a continuous monitoring of antimicrobial susceptibility among veterinary laboratories in European countries based on validated and harmonised methodologies. Available summary data of the susceptibility testing of the bacterial pathogens from the different laboratories were collected. Method: Antimicrobial susceptibility data for several bovine pathogens were obtained over a three year period (2002–2004). Each year the participating laboratories were requested to fill in excelfile templates with national summary data on the occurrence of antimicrobial resistance from different bacterial species. A proficiency test (EQAS – external quality assurance system) for antimicrobial susceptibility testing was conducted each year to test the accuracy of antimicrobial susceptibility testing in the participating laboratories. The data from this testing demonstrated that for the species included in the EQAS the results are comparable between countries. Results: Data from 25,241 isolates were collected from 13 European countries. For Staphylococcus aureus from bovine mastitis major differences were apparent in the occurrence of resistance between countries and between the different antimicrobial agents tested. The highest frequency of resistance was observed for penicillin. For Mannheimia haemolytica resistance to ampicillin, tetracycline and trimethoprim/sulphonamide were observed in France, the Netherlands and Portugal. All isolates of Pasteurella multocida isolated in Finland and most of those from Denmark, England (and Wales), Italy and Sweden were susceptible to the majority of the antimicrobials. Streptococcus dysgalactiae and Streptococcus uberis isolates from Sweden were fully susceptible. For the other countries some resistance was observed to tetracycline, gentamicin and erythromycin. More resistance and variation of the resistance levels between countries were observed for Escherichia coli compared to the other bacterial species investigated. Conclusion: In general, isolates from Denmark, England (and Wales), the Netherlands, Norway, Sweden and Switzerland showed low frequencies of resistance, whereas many isolates from Belgium, France, Italy, Latvia and Spain were resistant to most antimicrobials tested. In the future, data on the prevalence of resistance should be used to develop guidelines for appropriate antimicrobial use in veterinary medicine.
• Source

  Available from: Patrick Butaye

  Article: Occurrence of antimicrobial resistance among bacterial pathogens

  Rene S Hendriksen, Dik J Mevius, Andreas Schroeter, Christopher Teale, Eric Jouy, Patrick Butaye, Alessia Franco, Andra Utinane, Alice Amado, Miguel Moreno, Christina Greko, Katharina D.C. Stärk, Christian Berghold, Anna-Liisa Myllyniemi, Andrzej Hoszowski, Marianne Sunde, Frank M Aarestrup
  [show abstract] [hide abstract]
  ABSTRACT: Background: The project "Antibiotic resistance in bacteria of animal origin – II" (ARBAO-II) was funded by the European Union (FAIR5-QLK2-2002-01146) for the period 2003–05. The aim of this project was to establish a program for the continuous monitoring of antimicrobial susceptibility of pathogenic and indicator bacteria from food animals using validated and harmonised methodologies. In this report the first data on the occurrence of antimicrobial resistance among bacteria causing infections in pigs are reported. Methods: Susceptibility data from 17,642 isolates of pathogens and indicator bacteria including Actinobacillus pleuropneumoniae, Streptococcus suis and Escherichia coli isolated from pigs were collected from fifteen European countries in 2002–2004.Results: Data for A. pleuropneumoniae from infected pigs were submitted from five countries. Most of the isolates from Denmark were susceptible to all drugs tested with the exceptions of a low frequency of resistance to tetracycline and trimethoprim – sulphonamide. Data for S. suis were obtained from six countries. In general, a high level of resistance to tetracycline (48.0 – 92.0%) and erythromycin (29.1 – 75.0%) was observed in all countries whereas the level of resistance to ciprofloxacin and penicillin differed between the reporting countries. Isolates from England (and Wales), France and The Netherlands were all susceptible to penicillin. In contrast the proportion of strains resistant to ciprofloxacin ranged from 12.6 to 79.0% (2004) and to penicillin from 8.1 – 13.0% (2004) in Poland and Portugal. Data for E. coli from infected and healthy pigs were obtained from eleven countries. The data reveal a high level of resistance to tetracyclines, streptomycin and ampicillin among infected pigs whereas in healthy pigs the frequency of resistance was lower. Conclusion: Bacterial resistance to some antimicrobials was frequent with different levels of resistance being observed to several antimicrobial agents in different countries. The occurrence of resistance varied distinctly between isolates from healthy and diseased pigs, with the isolates from healthy pigs generally showing a lower level of resistance than those from diseased pigs. The study suggests that the choice of antimicrobials used for the treatment of diseased animals should preferably be based on knowledge of the local pattern of resistance.