Publications (3)3.39 Total impact
Article: Identification and characterization of a novel adenine phosphoribosyltransferase gene (ZmAPT2) from maize (Zea mays L.).[show abstract] [hide abstract]
ABSTRACT: Adenine phosphoribosyltransferase (APRT) is the key enzyme that converts adenine to adenosine monophosphate (AMP) in the purine salvage pathway. It was found that several different forms of APRT gene exist in plants, but no APRT gene in maize has been reported up to now. In this study, a novel maize APRT gene was cloned and characterized through a combination of bioinformatic, RT-PCR and RACE strategies. The full length of APRT cDNA sequence is 1202 nucleotides, with an ORF encoding 214 amino acid residues. Alignment of the deduced protein with that of other plant APRT genes indicates that the new gene is the form 2 of maize APRT, thus it was named ZmAPT2. Through basic local alignment search tool, search in the genomic survey sequence database of MaizeGDB, the putative genomic sequence of ZmAPT2 was obtained. Comparison of the cDNA and genomic sequence of the ZmAPT2 gene revealed that it contained seven exons and six introns. The locations of the introns within the maize ZmAPT2 coding region were consistent with those in the previously isolated APRTs of arabidopsis and rice. RT-PCR analysis showed that ZmAPRT was constitutively expressing in different organs under high temperature and salt stresses. Southern blot analysis indicated that at least three APRT genes existed in maize genome. These results confirmed that the novel maize ZmAPT2 gene was truly identified, and its potential role in maize growth and development was discussed.DNA Sequence 07/2008; 19(3):357-65. · 0.75 Impact Factor
Article: Characterization and fine mapping of RppQ, a resistance gene to southern corn rust in maize.[show abstract] [hide abstract]
ABSTRACT: Southern corn rust (SCR) is a fungal disease caused by Puccinia polysora Underw, which can infect maize and may result in substantial yield losses in maize production. The maize inbred line Qi319 carries the SCR resistance gene RppQ. In order to identify molecular markers linked to the RppQ gene, several techniques were utilized including random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP). In addition, sequence characterized amplified region (SCAR) techniques combined with bulked segregant analysis (BSA) were used. Seven RAPD markers, eight SSR markers, and sixty-three AFLP primer combinations amplified polymorphisms between two parents and two bulk populations. A large F2 population was used for genetic analysis and for fine mapping of the RppQ gene region. One AFLP polymorphic band, M-CAA/E-AGC 324, was converted to a SCAR marker, MA7, which was mapped to a position 0.46 cM from RppQ. Finally, the RppQ gene was mapped between the SCAR marker MA7 and the AFLP marker M-CCG/E-AGA 157 with distances of 0.46 and 1.71 cM, respectively.Molecular and General Genetics 01/2008; 278(6):723-8. · 2.63 Impact Factor
Article: Cloning a second form of adenine phosphoribosyl transferase gene (TaAPT2) from wheat and analysis of its association with thermo-sensitive genic male sterility (TGMS)[show abstract] [hide abstract]
ABSTRACT: Adenine phosphoribosyl transferase (APRT) is the key enzyme that converts adenine to adenosine monophosphate (AMP) in the purine salvage pathway. A novel form of APRT gene from wheat (Triticum aestivum L.) was cloned through a combination of bioinformatic, RT-PCR and rapid amplification of cDNA ends (RACE) strategies. The total length of the cDNA sequence is 1097 nucleotides, in which an open reading frame (ORF) was found that encodes 221 amino acid residues. Alignment of the deduced amino acid sequence and that of the other plant APRT genes indicates that the novel cDNA in full-length is the form 2 of wheat APRT gene, which was named as TaAPT2. Twelve Single Nucleotide Polymorphism (SNP) sites were identified in the TaAPT2 gene from BNY-S, one wheat temperature-sensitive genic male sterile (TGMS) mutant line derived from the wild type of BNY-F, among which a single base substitution in the purine-binding domain resulted in the Asn to Thr transition. The protein secondary structure prediction revealed that this SNP mutation subsequently resulted in many changes in the quantity and the positions of the helix, extended strand and the loop, which perhaps affect the combining efficiency of the APRT. Northern analysis indicated that the abundance of TaAPT2 gene transcript in the young spikelets reduced obviously in BNY-S under low temperature stress (lower than 10 °C) at the early stage of pollen fertility alternation, however, the transcript of TaAPT2 gene in BNY-F was not affected, indicating that wheat TaAPT2 gene may be related to the fertility alternation and abortion of pollen development.Plant Science.