Daonian Zhou

Fuerkang Beijing Institute of Biotechnology, Peping, Beijing, China

Are you Daonian Zhou?

Claim your profile

Publications (12)24.51 Total impact

  • Anhua Wei, Daonian Zhou, Guanghua Wu
    [Show abstract] [Hide abstract]
    ABSTRACT: A simple and sensitive HPLC method using UV detection was developed to determine the concentration of protoapigenone in rat plasma. Chromatographic separation was conducted on a C18 column with a mobile phase consisting of an acetonitrile-methanol-aqueous phase (containing 0.2% acetic acid, pH 3.0) system at a flow rate of 1.0 mL/min. The UV detector was set at 248 nm. The calibration curve was linear over the range of 0.031-10.0 µg/mL. The lower limit of quantification was 31 ng/mL. The recoveries for plasma samples ranged from 70.3 to 82.5%. The intra- and inter-day accuracy and precision fulfilled the international standards. This method was successfully applied to a pharmacokinetic study of protoapigenone in rats after oral administration of protoapigenone. It was shown that protoapigenone could be absorbed rapidly after oral administration and could reach the maximum concentration within 1 h. Copyright © 2013 John Wiley & Sons, Ltd.
    Biomedical Chromatography 07/2013; · 1.95 Impact Factor
  • Daonian Zhou, Anhua Wei, Cheng Cao, Jinlan Ruan
    [Show abstract] [Hide abstract]
    ABSTRACT: DICO was a novel nonaromatic B-ring flavonoid obtained from Macrothelypteris torresiana. In the present work, we investigated the antitumor activity and the antineoplastic mechanism of DICO. Our study showed that DICO inhibited the growth of HepG2 cells in dose and time-dependent manners. As well as DICO induced G2/M cell cycle arrest and apoptosis via a ROS-mediated mitochondrial pathway. Western blot assay demonstrated that DICO decreased Bcl-2 level and induced Bax translocation to cause cytochrome c release. Subsequently, caspase-9 and caspase-3 were activated. Meanwhile, the alterations of cyclin A and B1, p-CDK1 and p-cdc25c levels were also observed in response to DICO treatment. Taken together, DICO displayed a significant antitumor effect through G2/M cell cycle arrest and apoptosis induction, which suggested DICO might have therapeutic potential against tumors.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 04/2013; · 2.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Macrothelypteris viridifrons is widely distributed in south of China and has been used as folk medicine to treat cancer, hydropsy, and traumatic bleeding. To investigate the chemical constituents and the anti-tumor and anti-angiogenic effects of Macrothelypteris viridifrons. An HPLC-DAD/MS technique was used to determine the flavonoid profile of Macrothelypteris viridifrons. The anti-tumor effect of Macrothelypteris viridifrons was evaluated by in vivo mice bearing H22 hepatoma cells transplantation tumor model. And the anti-angiogenic activity was investigated by measuring the effects on the in vitro proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs). Furthermore, the in vivo zebrafish model was applied to evaluate the anti-angiogenic effect of Macrothelypteris viridifrons. 18 flavonoids were identified from Macrothelypteris viridifrons. Administration of Macrothelypteris viridifrons significantly inhibited the tumor growth and the expression of vascular endothelial growth factor (VEGF) and CD34. Meanwhile, Macrothelypteris viridifrons showed significant inhibition on proliferation, migration and tube formation of HUVECs in vitro and the intersegmental vessels formation in zebrafish model. Macrothelypteris viridifrons showed significant anti-tumor and anti-angiogenic effects and might be developed as a novel anti-tumor drug.
    Journal of ethnopharmacology 11/2011; 139(2):373-80. · 2.32 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim of the present study was to elucidate the chemical structure of a novel non-aromatic B-ring flavonoid (DHEC) isolated from Macrothelypteris viridifrons and to evaluate its putative molecular mechanism of action on induction of apoptosis in human colon HT-29 cancer cell. On the basis of MS, UV, IR, 1D and 2D NMR data, DHEC was identified as 2-(cis-1, 2-dihydroxy-4-oxo-cyclohex-5-enyl)-5-hydroxy-7-ethoxy-chromone. In addition, the cytotoxicity of DHEC and its effect on induction of apoptosis were confirmed by several assays. After treatment of HT-29 cell with DHEC, we observed the accumulation of intracellular reactive oxygen species, the loss of mitochondrial membrane potential, the alteration of expression of the Bcl-2 family members, the releasing of cytochrome c, the cleavage of poly (ADP-ribose) polymerase (PARP), and the activation of caspase-3, -8, and -9. Further analysis showed that the mitogen-activated protein kinase (MAPK) related proteins were stimulated by treatment with DHEC. These results suggest that DHEC exhibits potential anti-cancer activity in HT-29 cell through induction of apoptosis, which may highly be associated with reactive oxygen species-mitochondrial dysfunction as well as activation of MAPK signaling pathway.
    Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 09/2011; 49(9):2445-52. · 2.99 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To study the chemical constituents of Macrothelypteris viridifrons and their anti-proliferative effects on tumor cell. The compounds were isolated by column chromatography with silica gel, C18 reverse-phase silica gel, sephadex LH-20, and their structures were elucidated on the basis of physiochemical propertities and spectral analysis. The antitumor activities of all compounds were tested with MOLT4, Hep G2, A-549, MCF-7, HT-29, PC-3 tumor cell lines. Five compounds were isolated and identified as protoapigenone (1), protoapigenin (2), protoapigenin-4'-O-beta-D-glucopyanoside (3), 5,7-dihydroxy-2-(1,2-isopropyldioxy-4-oxo-cyclohex-5-enyl) -chromen-4-one (4), 5,7-dihydroxy-2-(1-hydroxy-2,6-dimethoxy-cyclohex-4-oxo) -chromen-4-one (5), respectively. All compounds were obtained from this plant for the first time. Compounds 1, 4 and 5 showed strong anti-proliferative effects on six tumor cells, which were in concentration-dependent manner.
    Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 03/2011; 36(5):582-4.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Two new flavone derivatives (1 and 2) were isolated from the aerial parts of Macrothelypteris torresiana, along with four known flavonoids: protoapigenin, apigenin, kaempferol and quercetin. The structures were determined on the basis of spectroscopic data. Compound 1 showed weak cytotoxic activity against human tumour cell lines HepG₂ , MCF₇ and K562.
    Natural product research 01/2011; 25(1):36-9. · 1.01 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The present study was conducted to evaluate the antioxidant, free radical scavenging, hepatoprotective and anti-inflammatory potential of Parathelypteris nipponica (Franch. et Sav.) Ching. Antioxidant activity of the methanolic extract of Parathelypteris nipponica (Franch. et Sav.) Ching (TMPN) was studied using in vitro and in vivo models, total flavonoids content in TMPN was found to be 262 +/- 5.6 mg/g (w/w). The TMPN exhibited strong antioxidant activity with EC50 values in reductive ability (0.18 +/- 0.02 mg/ml) and ferric thiocyanate (FTC) assay (0.10 +/- 0.01 mg/ml), strong free radical scavenging activity as evidenced by the low EC50 values in DPPH (1,1-diphenyl-2-picrylhydrazyl) (2.00 +/- 0.02 mg/ml), superoxide anion (0.60 +/- 0.05 mg/ml), OH radicals (0.26 +/- 0.03 mg/ml), and hydrogen peroxide (0.45 +/- 0.03 mg/ml) methods. Acute toxicity study revealed that the LD50 value of the extract was more than the dose 2000 mg/kg bodyweight of mice. Hepatoprotective activity of TMPN was determined by the carbon tetrachloride (CCl4)-induced oxidative tissue injury in rat liver, the extract showed significant hepatoprotective activity that was evident by enzymatic examination and histopathological study. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, the extract reduced carrageenan-induced rat paw oedema in dose-dependent manner, achieving high degree of anti-inflammatory activity. This study provides a scientific basis for the ethnomedical claims that Parathelypteris nipponica (Franch. et Sav.) Ching is effective against inflammation and liver injury.
    Journal of ethnopharmacology 08/2010; 130(3):521-8. · 2.32 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: The study was aimed to investigate the ethanol extract of Arachniodes exilis for the antioxidant and hepatoprotective activity. Antioxidant activity was evaluated by different assays, including reducing power, lipid peroxidation, 2, 2'-diphenyl-1-picrylhydrazyl (DPPH), 2, 2'-azinobis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), superoxide anion, hydroxyl radicals and hydrogen peroxide. The hepatoprotective activity of ethanol extract was studied on mice liver damage induced by CCL(4) by monitoring biochemical parameters. The extract showed potent activities on reducing power, lipid peroxide, DPPH, ABTS, superoxide anion, hydroxyl radical and hydrogen peroxide. And oral administration of Arachniodes exilis at different doses resulted in significant improvement on the levels of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase, malondialchehyche and superoxidedismutase. The results indicate that this plant possesses potential antioxidant and hepatoprotective properties and has therapeutic potential for the treatment of liver diseases.
    Journal of ethnopharmacology 03/2010; 129(2):232-7. · 2.32 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A new flavonoid, 5,7-dihydroxy-2-(1-hydroxy-2,6-dimethoxy-4-oxo-cyclohex)-chromen-4-one (1), was isolated from the roots of Macrothelypteris torresiana (Gaud.) Ching. (Thelypteridaceae). The structure of the product was identified on the basis of detailed spectral analysis, including X-ray structure analysis.
    Chemistry of Natural Compounds 01/2010; 46(2):209-211. · 0.60 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: A simple and reliable identification and quality control system has been developed for monitoring a herbal plant (Macrothelypteris torresiana) based on quantitative fingerprinting analysis using liquid chromatographic method with ultra-violet detector. The herbal extract was obtained by ultrasonic-assisted extraction. The separation was performed on a Burospher-100 C18 column by gradient elution with acetonitrile and aqueous phase (containing 0.5% H3PO4, pH 3.0) at a flow rate of 1.0mLmin−1. Under the optimal chromatographic condition, the relative standard deviations of the retention time and the peak area were less than 0.39 and 5.51%, respectively. The intra- and inter-day precisions were ranged from 0.76 to 3.23%. Good linear behaviors over the investigated concentration ranges were obtained with the values of R 2 higher than 0.999 for all analytes. The recoveries for spiked samples were in the range of 92.2–106.0%. Eleven peaks in the chromatograms of M. torresiana were identified for chromatographic fingerprint analysis. The proposed method was successfully applied to determine the contents of main constituents (protoapigenone, protoapigenin 4′-O-β-D-glucoside, apigenin 4′-O-β-D-glucoside, and apigenin) in different batches of M. torresiana. The proposed analytical procedure was proved to be a reliable and rapid method for the identification and quality control of the herbal plant M. torresiana.
    Chromatographia 01/2009; 70(1):117-124. · 1.44 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Six new flavanone glycosides (1-6) were isolated from the methanol extract of the rhizomes of Cyclosorus acuminatus, together with the parent flavanone glycoside 2a. Their structures were established on the basis of spectroscopic and chemical methods. All compounds showed moderate activity against Streptococcus pneumoniae and Haemophilus influenzae.
    Journal of Natural Products 12/2006; 69(11):1641-4. · 3.29 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Four new flavan-4-ol glycosides, abacopterins A-D (1-4), were isolated from a methanol extract of the rhizomes of Abacopteris penangiana, together with two known compounds, triphyllin A (5) and 6,8-dimethyl-7-hydroxy-4'-methoxyanthocyanidin-5-O-beta-D-glucopyranoside (6). Their structures were elucidated by extensive spectroscopic analysis and chemical methods. The cytotoxic activity of 1-5 against HepG2 human hepatoma cells was investigated.
    Journal of Natural Products 03/2006; 69(2):265-8. · 3.29 Impact Factor