D P Leitão

Publications (2)2.23 Total impact

• Source

  Available from: Vinícius Pedrazzi

  Article: Construction and evaluation of an inexpensive device that simulates oral clearance.

  A C Spadaro, D P Leitão, A C Polizello, V Pedrazzi, W Mestriner Júnior
  [show abstract] [hide abstract]
  ABSTRACT: The clearance pattern of a specific substance is very important to estimate its oral availability. Devices or models that simulate clearance in the mouth are important to study the effects and retention time of foods and drugs. This report describes an efficient device which was assembled with low-cost materials in our laboratory and that can be used to study the clearance of cariogenic substrates, mouthwashes, programmed-release drugs as well as adsorption of drugs to enamel. The device can have up to three chambers with varying minimum and maximum volumes that can be eluted simultaneously at different flows. The simulated swallowed volumes are adjustable and the ratio between the maximum and minimum volumes can be programmed. We also present the results of an evaluation study using the device to determine the clearance of fluoride from a fluoride-containing mouthwash, the clearance of a 1% glucose solution and the programmed release of fluoride from a methacrylate bioadhesive using artificial saliva as eluent.
  Brazilian Dental Journal 02/2001; 12(3):183-6.
• Article: Coagulation and fibrinolysis in capybara (Hydrochaeris hydrochaeris), a close relative of the guinea-pig (Cavia porcellus).

  D P Leitão, A C Polizello, Z Rothschild
  [show abstract] [hide abstract]
  ABSTRACT: Fibrinolytic and coagulation properties of capybara (Hydrochaeris hydrochaeris, LINNAEUS, 1766) plasma were analysed and the results compared to the guinea-pig (Cavia porcellus), a close relative. Capybara fibrinogen was isolated and fibrinolysis of its plasma was carried out in a homologous system and with bovine fibrin. Undiluted plasma did not have fibrinolytic activity on fibrin plates; euglobulins gave a dose-related response. Zymography of capybara and guinea-pig plasma gave the same patterns of activity as human or bovine plasma. Human urokinase (UK) and tissue plasminogen activator (t-PA) produced lysis in capybara fibrin plates. Streptokinase (SK) (500 IU/ml) did not activate capybara or guinea-pig plasma. In this system, human plasma was extensively activated. Coagulation tests for both species of rodent were prolonged. The capybara showed values for prothrombin time (PT) shorter than activated thromboplastin time (APTT). The guinea-pig, as already shown, had longer PT values. Factors X and VII were very low for capybara and guinea-pig when tested using reference curves and diagnostic kits for human plasma. It is suggested that the capybara could be a valuable laboratory animal considering its size and closeness to the guinea-pig, and this could allow for the provision of materials from one single animal when convenient or necessary.
  Comparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 02/2000; 125(1):113-20. · 2.23 Impact Factor