Chrisostomos I Dovas

Aristotle University of Thessaloniki, ThessalonĂ­ki, Kentriki Makedonia, Greece

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Publications (2)4.87 Total impact

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    ABSTRACT: ABSTRACT An isometric virus ca. 25 nm in diameter with angular contour was isolated from onion plants showing yellow leaf striping and necrotic tips. The virus was mechanically transmitted onto 28 species of indicator plants belonging to five families, viz. Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Leguminosae, and Solanaceae where it causes ring spots, malformations, and/or tip necrosis. Cytopathological studies in infected Nicotiana benthamiana tissues revealed cytoplasmic inclusions resembling those caused by Artichoke yellow ringspot virus (AYRSV), a member of the family Comoviridae. Host range and symptomatology of the onion virus were also similar to AYRSV. A high seed transmission rate (20%) was found in onion. Reverse transcription-polymerase chain reaction using degenerate primers specific for the family Comoviridae allowed amplification of RNA-dependent RNA polymerase sequences, which upon sequence analysis and comparison with AYRSV isolates from Cynara scolymus (AYRSV-AtG) and Vicia faba (AYRSV-F) were highly similar, thus providing evidence that the nepovirus AYRSV is infecting onion in the field.
    Phytopathology 07/2006; 96(6):622-9. · 2.97 Impact Factor
  • Chrisostomos I Dovas, Konstantinos Efthimiou, Nikolaos I Katis
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    ABSTRACT: A spot nested RT-PCR-RFLP method to detect and identify all members of the Tobamovirus genus is described. It involves a one-step RT-PCR, in which the combination of degenerate deoxyinosine (dI)-substituted primers amplified part of the polymerase region of tobamoviruses, followed by a nested PCR amplification that increased specificity and sensitivity of detection. Virus species differentiation was achieved by subsequent restriction enzyme analysis. The sensitivity of the method was increased further when along with one primer containing many dIs, another homologous primer in which dIs were substituted by dGs was used. The homologous primer was shorter than the dI-containing primer and with lower degeneracy, resulting in higher overall amplification efficiency due to the increased stability of the primer-target duplex. With this strategy, highly degenerate primers containing many dIs can be used effectively to improve detection sensitivity, alleviating problems of primer-target duplex destabilisation that can occur due to many dI substitutions. This method can be useful on the diagnosis, epidemiological investigation, and characterisation of known and unidentified Tobamovirus species.
    Journal of Virological Methods 06/2004; 117(2):137-44. · 1.90 Impact Factor