Charoula Psallida

N.AG.RE.F. - NATIONAL AGRICULTURAL RESEARCH FOUNDATION, Megalokastro, Crete, Greece

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Publications (3)5.36 Total impact

  • C. Psallida, C.G. Spyropoulos
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    ABSTRACT: Micropylar endosperm endo--mannanase of Lycopersicon esculentum (tomato) seeds hydrolyzes the mannan polysaccharides of the micropylar cell walls, allowing radicle protrusion. However, there is a controversy whether micropylar endo--mannanase is the limiting factor for tomato seed germination. In this report it is investigated whether endosperm endo--mannanase activity, which appeared during early tomato seed imbibition, was related to the germination ability of four cultivars (ACE, Olympia, Macedonia and Thessaloniki), the seeds of which were subjected to treatments that altered their germination behaviour and then germinated at three different temperatures. A high correlation (P Document Type: Research Article Publication date: October 1, 2006 More about this publication? Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality. Editorial Board Information for Authors Subscribe to this Title Membership Information ingentaconnect is not responsible for the content or availability of external websites $(document).ready(function() { var shortdescription = $(".originaldescription").text().replace(/\\&/g, '&').replace(/\\, '<').replace(/\\>/g, '>').replace(/\\t/g, ' ').replace(/\\n/g, ''); if (shortdescription.length > 350){ shortdescription = "" + shortdescription.substring(0,250) + "... more"; } $(".descriptionitem").prepend(shortdescription); $(".shortdescription a").click(function() { $(".shortdescription").hide(); $(".originaldescription").slideDown(); return false; }); }); Related content In this: publication By this: publisher In this Subject: Agriculture (General) By this author: Psallida, C. ; Spyropoulos, C.G. GA_googleFillSlot("Horizontal_banner_bottom");
    Seed Science and Technology 10/2006; 34(3). · 0.70 Impact Factor
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    ABSTRACT: A generic sample normalization method applicable in relative comparison of mRNAs quantified with real-time polymerase chain reaction (PCR) is proposed. The method was applied in samples obtained from tomato seeds after osmopriming and aging treatments and from untreated seeds at early imbibition stage, when seeds had not completed germination. Normalization in sample variations was accomplished by detecting synthetic DNA sequences tailing cDNA after second strand reverse transcription synthesis, while the use of the common normalizer GAPDH proved unreliable. Results, obtained from the new method and having a standard error less than 10%, verified the expression profile of a germination-specific mannanase gene that was closely recorded at different time intervals in relation to seed germination.
    FEBS Journal 03/2006; 273(4):770-7. · 4.25 Impact Factor
  • Charoula Psallida, Dimitris Argyropoulos
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    ABSTRACT: Messenger ribonucleic acid (mRNA) isolation from tomato seeds was performed with the PolyATtract System 1000 and was evaluated in two-step Real Time PCR using the fluoresence dye Sybr Green. The proposed isolation method resulted in cDNA production with Sybr Green 11-fold higher fluorescence intensity, having 87% lower standard deviation in the recorded fluorescence signals, and PCR efficiency improved by 21% in comparison to mRNA samples obtained with the manufacturer's protocol. The method for the assessment of the mRNA isolation procedure and the proposed mathematical analysis of the data are applicable to gene identification and expression studies for biotechnological applications.
    Preparative Biochemistry &amp Biotechnology 02/2005; 35(4):273-82. · 0.41 Impact Factor