Cathy Xiaoyan Zhong

Publications (2)12.99 Total impact

• Source

  Available from: Paul B Talbert

  Article: Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.

  Kiyotaka Nagaki, Paul B Talbert, Cathy Xiaoyan Zhong, R Kelly Dawe, Steven Henikoff, Jiming Jiang
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  ABSTRACT: The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.
  Genetics 04/2003; 163(3):1221-5. · 4.01 Impact Factor
• Source

  Available from: James A Birchler

  Article: Centromeric retroelements and satellites interact with maize kinetochore protein CENH3.

  Cathy Xiaoyan Zhong, Joshua B Marshall, Christopher Topp, Rebecca Mroczek, Akio Kato, Kiyotaka Nagaki, James A Birchler, Jiming Jiang, R Kelly Dawe
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  ABSTRACT: Maize centromeres are composed of CentC tandem repeat arrays, centromeric retrotransposons (CRs), and a variety of other repeats. One particularly well-conserved CR element, CRM, occurs primarily as complete and uninterrupted elements and is interspersed thoroughly with CentC at the light microscopic level. To determine if these major centromeric DNAs are part of the functional centromere/kinetochore complex, we generated antiserum to maize centromeric histone H3 (CENH3). CENH3, a highly conserved protein that replaces histone H3 in centromeres, is thought to recruit many of the proteins required for chromosome movement. CENH3 is present throughout the cell cycle and colocalizes with the kinetochore protein CENPC in meiotic cells. Chromatin immunoprecipitation demonstrates that CentC and CRM interact specifically with CENH3, whereas knob repeats and Tekay retroelements do not. Approximately 38 and 33% of CentC and CRM are precipitated in the chromatin immunoprecipitation assay, consistent with data showing that much, but not all, of CENH3 colocalizes with CentC.
  The Plant Cell 12/2002; 14(11):2825-36. · 8.99 Impact Factor