Bud Bromley

Publications (2)42.54 Total impact

• Source

  Available from: Thomas A. Cebula

  Article: The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements.

  Leming Shi, Laura H Reid, Wendell D Jones, Richard Shippy, Janet A Warrington, Shawn C Baker, Patrick J Collins, Francoise de Longueville, Ernest S Kawasaki, Kathleen Y Lee, [......], Alex Wong, Jie Wu, Chunlin Xiao, Qian Xie, Jun Xu, Wen Yang, Liang Zhang, Sheng Zhong, Yaping Zong, William Slikker
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  ABSTRACT: Over the last decade, the introduction of microarray technology has had a profound impact on gene expression research. The publication of studies with dissimilar or altogether contradictory results, obtained using different microarray platforms to analyze identical RNA samples, has raised concerns about the reliability of this technology. The MicroArray Quality Control (MAQC) project was initiated to address these concerns, as well as other performance and data analysis issues. Expression data on four titration pools from two distinct reference RNA samples were generated at multiple test sites using a variety of microarray-based and alternative technology platforms. Here we describe the experimental design and probe mapping efforts behind the MAQC project. We show intraplatform consistency across test sites as well as a high level of interplatform concordance in terms of genes identified as differentially expressed. This study provides a resource that represents an important first step toward establishing a framework for the use of microarrays in clinical and regulatory settings.
  Nature Biotechnology 10/2006; 24(9):1151-61. · 23.27 Impact Factor
• Article: The External RNA Controls Consortium: a progress report.

  Shawn C Baker, Steven R Bauer, Richard P Beyer, James D Brenton, Bud Bromley, John Burrill, Helen Causton, Michael P Conley, Rosalie Elespuru, Michael Fero, [......], Richard Shippy, Jesus V Soriano, Elizabeth A Wagar, Janet A Warrington, Mickey Williams, Frederike Wilmer, Mike Wilson, Paul K Wolber, Xiaoning Wu, Renata Zadro
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  ABSTRACT: Standard controls and best practice guidelines advance acceptance of data from research, preclinical and clinical laboratories by providing a means for evaluating data quality. The External RNA Controls Consortium (ERCC) is developing commonly agreed-upon and tested controls for use in expression assays, a true industry-wide standard control.
  Nature Methods 11/2005; 2(10):731-4. · 19.28 Impact Factor