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Bayin Chahan,
Zijian Jian,
Jilintai,
Kazuro Miyahara,
Shigeyuki Tanabe,
Xuenan Xuan,
Yukita Sato,
Tadaaki Moritomo,
Sadao Nogami,
Takeshi Mikami,
Soichi Maruyama,
Hisashi Inokuma
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ABSTRACT: Tick DNA samples from cattle in Xinjiang Uygur Autonomous Region Area, China, were examined for Rickettsia infection by citrate synthase gene-based PCR and sequencing. Four positive samples were detected from Haemaphysalis danieli and high levels of similarity were found with recently detected 'Candidatus Rickettsia principis.'
Veterinary Parasitology 04/2007; 144(1-2):184-7. · 2.58 Impact Factor
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Bayin Chahan,
Soufa Zhang,
Jin-Young Seo,
Chinatsu Nakamura,
Guohong Zhang,
Hiroshi Bannai,
Zijian Jian,
Hisashi Inokuma,
Kotaro Tuchiya,
Yukita Sato,
Hidenori Kabeya,
Soichi Maruyama,
Takeshi Mikami,
Xuenan Xuan
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ABSTRACT: The prevalence of Babesia (Theileria) equi and B. caballi infections in donkeys in western Xinjiang China was investigated. In total, 93 serum samples were randomly taken from donkeys in the Kashi and Ili areas, and examined for B. equi and B. caballi infections by enzyme-linked immunosorbent assays using recombinant antigens. Of the 93 samples, 9 (9.6%) and 36 (38.7%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 2 (2.2%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine babesiosis might be extensively prevalent in donkeys in western Xinjiang.
Journal of Veterinary Medical Science 08/2006; 68(7):753-5. · 0.85 Impact Factor
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Bayin Chahan,
Zijian Jian,
Xuenan Xuan,
Yukita Sato,
Hidenori Kabeya,
Kotaro Tuchiya,
Kazuhito Itamoto,
Masaru Okuda,
Takeshi Mikami,
Soichi Maruyama,
Hisashi Inokuma
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ABSTRACT: Serological methods were utilized to detect Anaplasma and Ehrlichia infection in domestic animals in Xinjiang Uygur Autonomous Region, China. By using an indirect immunofluorescence assay (IFA), antibodies that reacted with Anaplasmaphagocytophilum and Ehrlichiachaffeensis were detected mainly in ruminants kept on pastureland in Altai, Ili and Kashgar area. Antibody titers up to 1:320 were recorded. These results indicate that ruminants kept in these areas may be infected with some species of Anaplasma and Ehrlichia.
Veterinary Parasitology 01/2006; 134(3-4):273-8. · 2.58 Impact Factor
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Irungu Gaturaga, Bayin Chahan,
Xuenan Xuan,
Xiaohong Huang,
Min Liao,
Shinya Fukumoto,
Haruyuki Hirata,
Yoshihumi Nishikawa,
Yasuhiro Takashima,
Hiroshi Suzuki,
Kozo Fujisaki,
Chihiro Sugimoto
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ABSTRACT: The surface antigen 1-related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal signal peptide and C-terminal hydrophobic regions was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. ELISA could discriminate clearly between known N. caninum-positive and -negative sera from cattle. Field serum samples collected from cattle in Brazil were examined for the diagnosis of N. caninum infection using ELISA. Of the 197 samples analyzed, 64 (32.5%) samples were positive for antibodies to N. caninum. Of the 64 ELISA-positive samples, 58 (90.6%) were confirmed as positive by Western blot analysis with whole-parasite antigens. These results suggest that ELISA with recombinant NcSRS2t is an effective method for diagnosis of N. caninum infection in cattle.
Journal of Parasitology 03/2005; 91(1):191-2. · 1.40 Impact Factor
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Veterinary Parasitology - VET PARASITOL. 01/2005; 134:273-278.
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Bayin Chahan,
Irungu Gaturaga,
Xiaohong Huang,
Min Liao,
Shinya Fukumoto,
Haruyuki Hirata,
Yoshihumi Nishikawa,
Hiroshi Suzuki,
Chihiro Sugimoto,
Hideyuki Nagasawa,
Kozo Fujisaki,
Ikuo Igarashi,
Takeshi Mikami,
Xuenan Xuan
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ABSTRACT: Neospora caninum is a veterinary medically important pathogen capable of causing abortion in cattle and neuromuscular paralysis in dogs. The surface antigen 1 of N. caninum (NcSAG1) is an important candidate for the development of a diagnostic reagent for neosporosis. In order to establish an effective diagnostic method, the gene encoding truncated NcSAG1 (NcSAG1t) lacking a signal peptide and C-terminal hydrophobic regions was cloned and expressed in Escherichia coli as a fusion protein with glutathione S-transferase (GST). The purified GST-NcSAG1t was tested in an enzyme-linked immunosorbent assay (ELISA) for the detection of N. caninum antibodies in cattle. The ELISA with GST-NcSAG1t clearly differentiated between immunofluorescent antibody test (IFAT)-positive and -negative sera from cattle. In addition, the ELISA detected no cross-reactivity with sera from mice experimentally infected with the closely related parasite Toxoplasma gondii. Field serum samples collected from cattle in Brazil were examined for the diagnosis of neosporosis by using the ELISA. Of the 197 samples analyzed, 66 (33.5%) samples were positive for antibodies to N. caninum. Of the 66 ELISA-positive samples, 60 (90%) samples were confirmed as positive by Western blot analysis with whole parasite antigens. These results suggest that the recombinant NcSAG1t could be a reliable reagent for use as an antigen in ELISA for the serodiagnosis of N. caninum infection in cattle.
Veterinary Parasitology 01/2004; 118(3-4):177-85. · 2.58 Impact Factor
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ABSTRACT: The prevalence of equine piroplasmosis in Xinjiang province, China, was examined by enzyme-linked immunosorbent assays (ELISAs). A total of 70 serum samples were taken from horses pastured on three farms in western Xinjiang, and examined for diagnosis of equine Babesia equi (B. equi) infection and B. caballi infection by ELISAs using recombinant equi merozoite antigen 1 (EMA-1) and recombinant P48 antigen, respectively. Of the 70 samples, 28 (40.0%) and 17 (24.3%) samples were positive for B. equi infection and B. caballi infection, respectively. In addition, 11 (15.7%) samples were positive for both B. equi and B. caballi infections. These results indicate that equine piroplasmosis is widespread and therefore a cause for serious concern in western Xinjiang. To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China.
Veterinary Parasitology 10/2002; 108(2):179-82. · 2.58 Impact Factor
