Aurore Bogaert-Buchmann

Publications of Aurore Bogaert-Buchmann

• Cell uptake of a biosensor detected by hyperpolarized 129Xe NMR: the transferrin case.

  Authors: Céline Boutin, Antoine Stopin, Fatimazohra Lenda, Thierry Brotin, Jean-Pierre Dutasta, Nadège Jamin, Alain Sanson, Yves Boulard, François Leteurtre, Gaspard Huber, Aurore Bogaert-Buchmann, Nawal Tassali, Hervé Desvaux, Marie Carrière, Patrick Berthault

  Bioorganic & medicinal chemistry. 07/2011; 19(13):4135-43.

  For detection of biological events in vitro, sensors using hyperpolarized (129)Xe NMR can become a powerful tool, provided the approach can bridge the gap in sensitivity. Here we propose constructsFor detection of biological events in vitro, sensors using hyperpolarized (129)Xe NMR can become a powerful tool, provided the approach can bridge the gap in sensitivity. Here we propose constructs based on the non-selective grafting of cryptophane precursors on holo-transferrin. This biological system was chosen because there are many receptors on the cell surface, and endocytosis further increases this density. The study of these biosensors with K562 cell suspensions via fluorescence microscopy and (129)Xe NMR indicates a strong interaction, as well as interesting features such as the capacity of xenon to enter the cryptophane even when the biosensor is endocytosed, while keeping a high level of polarization. Despite a lack of specificity for transferrin receptors, undoubtedly due to the hydrophobic character of the cryptophane moiety that attracts the biosensor into the cell membrane, these biosensors allow the first in-cell probing of biological events using hyperpolarized xenon.

• Sensitivity and multiplexing capabilities of MRI based on polarized 129Xe biosensors.

  Authors: Patrick Berthault, Aurore Bogaert-Buchmann, Hervé Desvaux, Gaspard Huber, Yves Boulard

  Journal of the American Chemical Society. 01/2009; 130(49):16456-7.