Anna A Lazareva

Publications (2)18.38 Total impact

• Article: The circadian output gene takeout is regulated by Pdp1epsilon.

  Juliana Benito, Valbona Hoxha, Chamala Lama, Anna A Lazareva, Jean-François Ferveur, Paul E Hardin, Brigitte Dauwalder
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  ABSTRACT: The circadian clock controls many circadian outputs. Although a large number of transcripts are affected by the circadian oscillator, very little is known about their regulation and function. We show here that the Drosophila takeout gene, one of the output genes of the circadian oscillator, is regulated similarly to the circadian clock genes Clock (Clk) and cry. takeout RNA levels are at constant high levels in Clk(JRK) mutants. The circadian transcription factor PAR domain protein 1 (Pdp1epsilon) is a transcription factor that had previously been postulated to control clock output genes, particularly genes regulated similarly to Clk. In agreement with this, we show here that Pdp1epsilon is a regulator of takeout. Takeout levels are low in flies with reduced Pdp1epsilon and high in flies with increased amounts of Pdp1epsilon. Furthermore, flies with reduced or elevated Pdp1epsilon levels in the fat body display courtship defects, identifying Pdp1epsilon as an important transcriptional regulator in that tissue.
  Proceedings of the National Academy of Sciences 02/2010; 107(6):2544-9. · 9.68 Impact Factor
• Source

  Available from: PubMed Central

  Article: A role for the adult fat body in Drosophila male courtship behavior.

  Anna A Lazareva, Gregg Roman, William Mattox, Paul E Hardin, Brigitte Dauwalder
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  ABSTRACT: Mating behavior in Drosophila depends critically on the sexual identity of specific regions in the brain, but several studies have identified courtship genes that express products only outside the nervous system. Although these genes are each active in a variety of non-neuronal cell types, they are all prominently expressed in the adult fat body, suggesting an important role for this tissue in behavior. To test its role in male courtship, fat body was feminized using the highly specific Larval serum protein promoter. We report here that the specific feminization of this tissue strongly reduces the competence of males to perform courtship. This effect is limited to the fat body of sexually mature adults as the feminization of larval fat body that normally persists in young adults does not affect mating. We propose that feminization of fat body affects the synthesis of male-specific secreted circulating proteins that influence the central nervous system. In support of this idea, we demonstrate that Takeout, a protein known to influence mating, is present in the hemolymph of adult males but not females and acts as a secreted protein.
  PLoS Genetics 02/2007; 3(1):e16. · 8.69 Impact Factor