[show abstract][hide abstract] ABSTRACT: We applied PCR–RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) analysis to identify seven gadoid
species of different biogeographical origin and commercial relevance, namely Gadus morhua (Atlantic ocean); Trisopterus minutus capelanus, Trisopterus minutus minutus, Molva elongata, Phycis blennoides, Micromesistius poutassou (Atlantic ocean and Mediterranean sea); Theragra chalcogramma (Pacific ocean). Two DNA fragments belonging to mitochondrial 12S and 16S rRNA genes of about 430 and 630bp, respectively,
were isolated by PCR amplification. Their direct sequencing showed a significant genotypic diversity among gadoid species,
useful for species identification. Digestion of 16S rRNA gene PCR fragment with MvaI or Bsh1285I restriction enzymes, followed by agarose gel electrophoresis of the cleaved products, yielded specific restriction profiles
that enabled direct, visual identification of the species analyzed. This PCR–RFLP method allowed a clear and rapid discrimination
of the gadoid species studied.
European Food Research and Technology 04/2012; 225(3):337-344. · 1.44 Impact Factor
[show abstract][hide abstract] ABSTRACT: A study on the oxidative defenses during larval growth and under stress conditions was carried out in the bred sea bass, Dicentrarchus labrax. A high-pressure liquid chromatography (HPLC) method was used for the quantitative determination of vitamins C and E; glutathione peroxidase activity was measured by an enzymatic assay. Vitamin E was measured in the seminal fluid, eggs, embryos and larvae. Vitamins C and E, and glutathione peroxidase activity were measured in adults of Dicentrarchus labrax under normal conditions and subjected to hypoxia. Vitamin E content was high in seminal fluid, eggs, and embryos and at the early stage of larval development. It decreased slowly, but steadily, throughout the larval growth. In adults exposed to hypoxia, vitamins C and E levels were significantly lower with respect to the control group. Glutathione peroxidase levels showed a decrease in the hypoxia-subjected group, although the values were not significant.
Advances in experimental medicine and biology 02/2003; 530:681-8. · 1.83 Impact Factor
[show abstract][hide abstract] ABSTRACT: Antioxidant plasma activities of ascorbate, alpha-tocopherol and glutathione peroxidase were analysed in adult male sea bass, Dicentrarchus labrax, in normal conditions and after hypoxia-recovery. In addition, tank measurements of temperature, pH, salinity and chlorine changes were carried out. Ascorbate and alpha-tocopherol were measured using a high-pressure liquid chromatography method and glutathione peroxidase activity enzymatically. Ascorbate and alpha-tocopherol showed a relationship with the velocity of body growing in normal and hypoxia-recovery conditions. In sea bass exposed to hypoxia, only ascorbate and alpha-tocopherol levels were significantly lower compared with the control group. Slope study and expression percent of antioxidants reduction after stress conditions revealed a predominant role of plasma alpha-tocopherol. Sea bass subjected to variations of salinity and chlorine showed a significant decrease in plasma alpha-tocopherol. A relationship could be suggested between antioxidant defence and fish response in aquaculture.
Comparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 06/2002; 132(1):205-11. · 2.17 Impact Factor