A. A. Shapovalova

Winogradsky Institute Of Microbiology, Moskva, Moscow, Russia

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Publications (3)6.29 Total impact

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    A. A. Shapovalova · T. V. Khijniak · T. P. Tourova · D. Yu. Sorokin ·
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    ABSTRACT: A heterotrophic bacterial strain AGD 8-3 capable of denitrification under extreme haloalkaline conditions was isolated from soda solonchak soils of the Kulunda steppe (Russia). The strain was classified within the genus Halomonas. According to the results of 16S rRNA gene sequencing, Halomonas axialensis, H. meridiana, and H. aquamarina are most closely related to strain AGD 8-3 (96.6% similarity). Similar to other members of the genus, the strain can grow within a wide range of salinity and pH. The strain was found to be capable of aerobic reduction of chromate and selenite on mineral media at 160 g/l salinity and pH 9.5–10. The relatively low level of phylogenetic similarity and the phenotypic characteristics supported classification of strain AGD 8-3 as a new species Halomonas chromatireducens.
    Microbiology 01/2009; 78(1):102-111. DOI:10.1134/S0026261709010135 · 0.64 Impact Factor
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    A Chmura · A A Shapovalova · S van Pelt · F van Rantwijk · T P Tourova · G Muyzer · D Yu Sorokin ·
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    ABSTRACT: An enrichment culture from saline soda soils, using acetate as carbon and energy source and 2-phenylpropionitrile as nitrogen source (PPN) at pH 10, resulted in the isolation of strain ANL-alpha CH3. The strain was identified as a representative of the genus Halomonas in the Gammaproteobacteria. The bacterium was capable of PPN utilization as a nitrogen source only, while phenylacetonitrile (PAN) served both as carbon, energy and nitrogen source. This capacity was not described previously for any other haloalkaliphilic bacteria. Apart from the nitriles mentioned above, resting cells of ANL-alpha CH3 also hydrolyzed mandelonitrile, benzonitrile, acrylonitrile, and phenylglycinonitrile, presumably using nitrilase pathway. Neither nitrile hydratase nor amidase activity was detected. The isolate showed a capacity to grow with benzoate and salicylate as carbon and energy source and demonstrated the ability to completely mineralize PAN. These clearly indicated a potential to catabolize aromatic compounds. On the basis of unique phenotype and distinct phylogeny, strain ANL-alpha CH3 is proposed as a novel species of the genus Halomonas--Halomonas nitrilicus sp. nov.
    Applied Microbiology and Biotechnology 10/2008; 81(2):371-8. DOI:10.1007/s00253-008-1685-x · 3.34 Impact Factor
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    AA Shapovalova · T V Khijniak · T P Tourova · G Muyzer · DY Sorokin ·
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    ABSTRACT: In this paper we describe denitrification at extremely high salt and pH in sediments from hypersaline alkaline soda lakes and soda soils. Experiments with sediment slurries demonstrated the presence of acetate-utilizing denitrifying populations active at in situ conditions. Anaerobic enrichment cultures at pH 10 and 4 M total Na+ with acetate as electron donor and nitrate, nitrite and N2O as electron acceptors resulted in the dominance of Gammaproteobacteria belonging to the genus Halomonas. Both mixed and pure culture studies identified nitrite and N2O reduction as rate-limiting steps in the denitrification process at extremely haloalkaline conditions. Electronic supplementary material The online version of this article (doi:10.1007/s00792-008-0166-6) contains supplementary material, which is available to authorized users.
    Extremophiles 06/2008; 12(5):619-25. DOI:10.1007/s00792-008-0166-6 · 2.31 Impact Factor