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Publications (11)2.48 Total impact

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    ABSTRACT: Pulmonary sarcoidosis may progress to fibrosis in some patients, so that close monitoring of its activity is essential for recommending clinical strategy. Examination of airway inflammatory markers in bronchoalveolar lavage (BAL) is one of the methods applied to assess the disease severity. Recently, the expired breath condensate (EBC) has become another source of cytokines and mediators. In sarcoidosis, except for NO and oxidative stress markers, no other mediators have yet been estimated in the exhaled air. In the present study we attempted to answer the question of whether airway inflammatory markers in pulmonary sarcoidosis patients might be assessable in EBC and to what extend these markers might reflect the disease activity in the lungs IL-6, TNF-alpha, PAI-1, and IGF-1 were measured by Elisa method in EBC and BALF samples from 9 patients with newly-diagnosed pulmonary sarcoidosis. TNF-alpha, IGF-1, and PAI-1 levels in EBC and BAL samples were comparable and closely positively correlated [TNF-alpha (r=0.79, P<0.001), IGF-1 (r=0.94, P<0.001), and PAI-1 (r=0.81, P<0.001)]. In contrast, IL-6 concentration in EBC was significantly lower compared with that in BALF, while the correlation between both materials was negative (r=-0.47, P<0.05). An important distinction in IL-6 performance, which might explain this inconsistency, is its tendency to form more complex molecular forms of a higher weight than that of other cytokines. Our study shows that EBC reflects cytokine production in the lung as effectively as BALF, providing that the characteristics of proteins evaluated allow their easy transfer into the exhaled air. Further studies are required before accepting EBC samples as an equivalent to BALF.
    Journal of physiology and pharmacology: an official journal of the Polish Physiological Society 09/2006; 57 Suppl 4:335-40. · 2.48 Impact Factor
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    ABSTRACT: The aim of the study was to evaluate factors that could predict smoking cessation after a minimal antismoking counseling during spirometric screening for COPD. Every subject filled-in a simple questionnaire on clinical signs of COPD and tobacco habit, had a spirometry performed according to ATS standards and received a short antitobacco counseling together with a booklet on how to quit smoking. Out of 800 smokers over 40 years of age, smoking history of more than 10 packyears, screened for COPD in 1999, four hundred were invited a year later for a follow-up spirometry and evaluation of anti-smoking intervention. Of 383 patients, who responded to the invitation (208 M and 175 F, mean age 56.6 +/- 10.7 yrs), 52 (13.6%) quit smoking for one year and another 48 (12.5%) quit smoking temporarily and than resumed smoking. Smokers who permanently succeeded in quitting smoking were older (60.5 vs 55.9 years p < 0.01), started smoking later (age at starting smoking 22 vs 19.5 years p < 0.001), had a shorter tobacco exposition (28.8 vs 34.3 packyears p < 0.05), had lower lung function (FEV1%pred 80.5 vs 89.2% p < 0.05) and were less nicotine dependent (FTQ score 1 vs 4.8 p < 0.00001).
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 01/2001; 69(11-12):611-6.
  • E Puścińska, A Goljan
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/2000; 68(11-12):491-7.
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    ABSTRACT: The aim of this study was to analyze the association between HLA class I and class II DR frequency in the different stages of sarcoidosis in Polish population. 88 patients and 30 healthy controls have been typed. Patients were divided into three groups depending on radiological findings. In the first group were 28 cases presenting the regression of the disease. In the second were 33 patients in stable stage II or III and in the third group 27 patients with pulmonary fibrosis (stage IV). The typing was performed by NIH method using commercial sera. There were no statistically significant differences between studied group in HLA-A class I. The frequency of HLA B-18 was statistically more frequent in patients with sarcoidosis compared to healthy controls. HLA-DR1 was not present in third group of patients and the difference was significant compared to healthy controls.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/2000; 68(11-12):498-509.
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    ABSTRACT: The aim of this study was evaluation of the significance of HLA-DRB1,-DRB3,-DRB4,-DRB5 associated alleles in the genetic susceptibility to sarcoidosis. We investigated 17 Polish 'families with' familial occurrence of the disease. Thirty three affected family members and 78 healthy relatives and 101 healthy individuals (control group) have been typed for HLA class II DR antigens. Assuming that relatives from families with two or more affected members are more susceptible to develop sarcoidosis we considered two classes: affected and healthy family members taken together (class 1, N = 111) and healthy control group (class 2, N = 101). HLA antigens from both classes were compared using a statistical pattern recognition method. This method, called k-NN method, assumes that objects (individuals) are described by a certain number of variables called features. Selected features that played an important role in the decision to which class an individual person would be classified were: HLA-DR7, HLA-DR2(16), HLA-DR1, HLA-DR5(12), HLA-DR6(14), HLA-DR6(13), HLA-DR9, HLA-DR5(11) and HLA-DRw52. K-NN method allowed to classify properly 76% of studied subjects to healthy or disease susceptible group. However, 24 out of 100 individuals would be misclassified which gives the total error rate of 0.24. We concluded that using HLA-DR antigens as features characterising every individual we can predict with high probability to which class ("high risk" or "healthy") individual would belong.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/2000; 68(11-12):545-56.
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    ABSTRACT: Sarcoidosis is a systemic disease of undetermined etiology although it can be related to genetic or environmental factors or both. We investigated 19 polish caucasian families with at least two affected relatives and healthy families members in order to evaluate the clinical aspects of familial sarcoidosis and the influence of environmental factors. We have found three types of familial sarcoidosis: 10 sib pairs, 1 sib triplet, 5 parent offspring pairs and one case of sarcoidosis in cousins. Most of sarcoidosis cases were histologically proved. A high frequency of chronic onset of the disease, chronic form with extrapulmonary sarcoidosis and fibrosis were observed. In only three families the course of the disease was similar in both affected relatives. There was no specific environmental agent found that could be related to the development of the disease. We concluded that it may be possible that familial sarcoidosis can have poorer prognosis that non-familial form, but there is no epidemiological data available about the clinical aspects of sarcoidosis in Poland. The clinical aspects of familial sarcoidosis can suggest the inherited susceptibility to the disease. The etiologic extrinsic factor has not been identified, but it doesn't exclude its role in the pathogenesis.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/2000; 68(11-12):510-22.
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    ABSTRACT: Several studies suggested association between some human leukocyte antigen (HLA) alleles and sarcoidosis, but none has been conclusive. To confirm possible association of sarcoidosis with HLA-DRB1, -DRB3,- DRB4, -DRB5 associated alleles HLA-DR genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 17 polish families with familial sarcoidosis and in 101 healthy controls. The families with sarcoidosis consisted of 31 affected first-degree relatives from 16 families, 2 affected cousins from 1 family and 78 healthy relatives of those patients. We found 3 varieties of familial sarcoidosis: a) in parent and offspring (5 pairs), b) in siblings (10 sib pairs and 1 sib triplet) and in cousins (1 family). Genotyping for HLA-DRB1,-DRB3,-DRB4,-DRB5 revealed an over-representation of HLA-DR5(12) and DRw52 among antigens shared by affected relatives comparing to the control group. A significant increase in the frequency of HLA-DR7 and HLA-DRw53 antigens (p < 0.05) was found in subjects from the control group. Comparing the group of family members (affected and healthy relatives taken together, n = 111) with the control group (n = 101) we found a significant differences in the distribution of HLA-DR2(15), HLA-DR5(12), HLA-DR6, HLA-DR9 and HLA-DRw52. Those antigens were more frequent (p < 0.05) in members from families with sarcoidosis. The frequencies of HLA-DR1, HLA-DR2(16), HLA-DR7 and HLA-DRw53 were significantly higher (p < 0.05) in the control group. Presented results suggest that HLA-DRB alleles contribute to the susceptibility to sarcoidosis in the Polish population.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 01/2000; 68(11-12):533-44.
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    ABSTRACT: Thirty three affected members from 17 Polish sarcoidosis families and their healthy relatives (61 subjects) have been typed for HLA class I (A and B). Controls consisted of 101 healthy Polish subjects. The HLA typing was performed by the serological method using the standard microcytotoxicity test. No significant differences were observed between group 1 (33 affected family members), group 2 (61 healthy relatives) and group 3 (healthy controls) considering the HLA class I locus A antigens. The frequencies of HLA B8, HLA B16 and HLA B40 were significantly higher in affected sarcoidosis families members compared to control subjects (p < 0.05). When comparing all families members (affected and non-affected as a one group) to the control group we found a significant overrepresentation of HLA B12 and B35 in the control group. We concluded that HLA antigens locus B: B12 and B35 may have a protective function against the disease; the role of HLA class I antigens in the pathogenesis of sarcoidosis needs further evaluation.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 01/2000; 68(11-12):523-32.
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    ABSTRACT: The deletorious health effects of smoking are generally known. In spite of that, great numbers of people still smoke tobacco in the whole world. It is primarily due to the addictive properties of nicotine. Cigarette smoking is also dependent on various social and psychologic factors making quitting very difficult. Among various treatment modalities for tobacco dependence we aimed to assess the efficacy of nicotine replacement therapy (NRT) vs group therapy. 325 subjects smoking at least 15 cigarettes/day for more than 3 years were studied. They were allocated to group therapy (neurolinguistic programming) or NRT (gum or patch) at their will. Non-smoking was validated at each of follow-up visits, at 1 and 2 weeks 1, 3, 6, 12 months by measuring CO in expired air. All groups were matched in age, smoking history and nicotine dependence. The best quit rate was observed as a result of group therapy (41% at 1 year, p. < 0.001) as compared to nicotine patch (2%) and nicotine gum (9%).
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/1999; 67(3-4):95-102.
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    ABSTRACT: From 1960 to 1994 2150 sarcoid patients were observed in our Sarcoidosis Clinic. Before the diagnosis of sarcoidosis was confirmed, 52 of those patients were treated with tuberculostatics because of the radiological changes in the lung, diagnosed as tuberculosis. In no case any conventional method-smear examination for acid bacilli or culture identification was positive. There was no radiological improvement after treatment in any patient. Subsequently in all those patients sarcoidosis was diagnosed, by typical histology (48 cases) or on clinical grounds (4 patients). In 15 cases spontaneous remission was observed. 37 patients were treated with steroids and in 31 of them radiological improvement was found. In six cases stabilization of the disease was noted. The observation time after steroids treatment was from one year to 27 years. In only one case tuberculosis of the lung has developed.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 02/1996; 64(5-6):261-6.
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    ABSTRACT: Although very little is known about the aetiology of sarcoidosis, its immunopathogenesis is now better known. The interaction of alveolar macrophages and T cells may play a role in the pathomechanism of the disease. To infiltrate the tissue, lymphocytes have to migrate through the subendothelial basement membrane and interstitium, rich in extracellular matrix (ECM). The interaction of lymphocytes with proteins of the ECM may play an important role in the migration, accumulation and activation of these cells. The aim of our study was to estimate the ability of the ECM components (collagen I, collagen IV and fibronectin) to co-stimulate T-cells in patients with sarcoidosis. The peripheral blood was obtained from 14 sarcoid patients. The disease was confirmed histologically in 9 cases and in 5 patients on clinical grounds. In radiological findings 4 persons were at the first stage of the disease, 4 at the second, in three cases interstitial changes were found and in three patients, the fibrosis on the X-ray was noticed. No one of those patients were treated with steroids during last 2 months. Normal peripheral blood T cells are strongly co-stimulated by ECM proteins. In contrast, lymphocytes from patients with sarcoidosis were inhibited by the ECM proteins. The mean co-stimulation ratios (OKT3 + ECM proteins:OKT3 alone) were significantly lower for all ECM proteins (collagen I: p < 0.00009; collagen IV: p < 0.02; fibronectin: p < 0.04). Our data shed a new light on the nature of sarcoidosis associated immunodeficiency and suggest that disturbed T cells: ECM interactions may play a role in the pathogenesis.
    Pneumonologia i alergologia polska: organ Polskiego Towarzystwa Ftyzjopneumonologicznego, Polskiego Towarzystwa Alergologicznego, i Instytutu Gruzlicy i Chorob Pluc 01/1996; 64(9-10):671-8.