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Ceskoslovenská oftalmologie 10/1986; 42(5):322-30.
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Ceskoslovenská oftalmologie 10/1986; 42(5):315-21.
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Ceskoslovenská oftalmologie 04/1985; 41(2):101-6.
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ABSTRACT: In order to assess the extent of injury and the response of the cornea to alkali burns, NaOH in concentrations of 0.5, 0.25, 0.1, 0.05, and 0.01 N was applied to rabbit eyes and the histologic and metabolic changes studied. The activities of alkaline and acid phosphatases in homogenates and in cold microtome sections were examined on days 1, 4, and 7 after injury. At all time intervals 0.5 N and 0.25 N NaOH induced a remarkable decrease in enzyme activities. On the other hand, after 0.05 N and 0.01 N NaOH only very slight changes were observed. Using 0.1 N NaOH, both phosphatases decreased on day 4 after treatment and acid phosphatase reached normal values in a week, whereas alkaline phosphatase increased with a maximum on day 7. Its role in synthetic processes during corneal regeneration is discussed. Both histologic and metabolic patterns in the experimentally burned cornea were shown to be a function of NaOH concentration and the duration of contact. The process of re-epithelization of the cornea during healing after 0.1 N NaOH for 1 min is described.
Albrecht von Graæes Archiv für Ophthalmologie 02/1984; 222(2):86-9. · 2.17 Impact Factor
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ABSTRACT: Alkaline and acid phosphatases were studied in the rabbit cornea following acetone and ethanol exposure to the eye. Changes in enzyme activities were investigated in homogenates of epithelium and stroma quantitatively and in frozen cryostat sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings showed a remarkable increase in alkaline phosphatase of the epithelium beginning on day 7. This activation persisted until day 28 after instillation of both noxae with maximum activity on day 14. However, acetone was proved to be significantly more effective than ethanol. In addition, a different topochemistry of alkaline phosphatase was found in the epithelium of treated corneas, i.e., enzyme activity was observed not only superficially but in all epithelial layers of the cornea as compared to a normal one. The effect of acetone and ethanol on a regenerating corneal epithelium is discussed.
Albrecht von Graæes Archiv für Ophthalmologie 02/1983; 220(2):96-9. · 2.17 Impact Factor
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Ceskoslovenská oftalmologie 04/1982; 38(2):76-81.
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Ceskoslovenská oftalmologie 12/1979; 35(6):415-20.
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ABSTRACT: Activities of alkaline and acid phosphates were investigated in rabbit corneas after a complete epithelial denudation in vivo (limbus to limbus). Dynamics of enzymatic changes during corneal healing were followed quantitatively in homogenates of regenerated epithelium and stroma (denuded cornea) and in cryostate frozen sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings at these times showed a different response in each enzyme. Acid phosphatase displayed a gradual increase of activity in epithelium as well as in stroma; on day 28 after injury its content was normal. In contrast, alkaline phosphatase showed delayed activity during the repair process, and even a month after de-epithelization was still subnormal, particularly in superficial layers of epithelium.
Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 01/1979; 209(2):137-43.
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Ceskoslovenská oftalmologie 02/1978; 34(1):3-9.
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Ceskoslovenská oftalmologie 12/1977; 33(6):397-401.
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ABSTRACT: The distribution of alkaline phosphatase was investigated in corneas of various animals by means of histochemical and biochemical methods. Special attention was paid to keratocytes that proved to be positive when a proper substrate and technique were used even if the activity differed according to animal species. Naphthol-AS-MX-phosphatate with Variamine-Blue-RT salt in the simultaneous azocoupling method was the most sensitive substrate; less suitable were naphthol-AS-phosphate, and particularly l-naphthyl-phosphate with Fast-Blue-BB-salt in the same method. Keratocytes were completely negative with beta-glycerophosphate in the Gomori technique. Contrary to keratocytes, epithelial and endothelial cells were without substrate predilection. The results of both approaches showed a wide inter-species variability. The overall activity of alkaline phosphatase detected histochemically and its activity in the unsedimentable fraction of whole corneas determined biochemically was in good agreement. The highest activity was found in the calf cornea; enzyme levels decreased gradually in the bovine, rabbit, dog, sheep, and pig cornea.
Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 12/1977; 204(3):209-14.
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A Bolková
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ABSTRACT: The activities of acid phosphatase, beta-glucuronidase, beta-galactosidase, and N-acetyl-beta-D-glucosaminidase were investigated in the normal rabbit cornea. For all spectrophotometric assays, appropriate p-nitrophenyl derivates were used. Only beta-glucuronidase were determined employing phenolphthalein glucuronid as a substrante. Acid phosphatase revealed the highest activity, followed by N-acetyl-beta-D-glucosaminidase and beta-galactosidase. In the case of beta-glucuronidase the lowest activity was found. The results on the rabbit cornea are compared with those on some other tissues described in the literature. Correlation of biochemical data and histochemical findings in the same species is briefly discussed.
Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 08/1977; 203(1):67-71.
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ABSTRACT: In the rabbit and bovine cornea the activity of alkaline phosphatase using histochemical as well as biochemical methods was investigated. Biochemically the enzyme activity was studied in separated corneal layers. In the histochemical investigation the best results were obtained in cryostat sections using the azocoupling method with naphthol AS-MX phosphate and Variamine Blue RT Salt. The enzyme activity was found not only in the epithelium and endothelium (as was described previously) but even in keratocytes. The mutual relation of activities in the epithelium and in keratocytes differed in both species. The overall activity found by histochemical methods is in good agreement with the biochemical determination of alkaline phosphatase (p-nitrophenyl phosphate as the substrate). Besides the histochemical approach shows an uneven distribution of alkaline phosphatase activity in individual cells which cannot be assessed by the biochemical determination.
Histochemistry 11/1976; 49(3):237-43.
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ABSTRACT: In the rabbit and bovine cornea the activity of alkaline phosphatase using histochemical as well as biochemical methods was investigated. Biochemically the enzyme activity was studied in separated corneal layers. In the histochemical investigation the best results were obtained in cryostat sections using the azocoupling method with naphthol AS-MX phosphate and Variamine Blue RT Salt. The enzyme activity was found not only in the epithelium and endothelium (as was described previously) but even in keratocytes. The mutual relation of activities in the epithelium and in keratocytes differed in both species. The overall activity found by histochemical methods is in good agreement with the biochemical determination of alkaline phosphatase (p-nitrophenyl phosphate as the substrate). Besides the histochemical approach shows an uneven distribution of alkaline phosphatase activity in individual cells which cannot be assessed by the biochemical determination.
Histochemie 08/1976; 49(3):237-243. · 2.59 Impact Factor
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Ceskoslovenská oftalmologie 08/1976; 32(4):252-7.
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ABSTRACT: Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.
Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 02/1976; 200(3):251-5.
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Ceskoslovenská oftalmologie 08/1975; 31(4):291-9.
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Ceskoslovenská oftalmologie 08/1974; 30(4):303-11.
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Ceskoslovenská oftalmologie 06/1974; 30(3):175-80.
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Ceskoslovenská oftalmologie 06/1974; 30(3):171-4.
