A Bolková

Charles University in Prague, Praha, Praha, Czech Republic

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Publications (36)17.84 Total impact

  • J Cejková, A Bolková
    Ceskoslovenská oftalmologie 10/1986; 42(5):322-30.
  • A Bolková, J Cejková
    Ceskoslovenská oftalmologie 10/1986; 42(5):315-21.
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    ABSTRACT: In rabbits inflammation of the cornea was induced by intrastromal injection of horse serum. Between 2 and 4 weeks after injection, infiltration of the cornea with leukocytes and neovascularization could be observed. During this period, the rabbits were killed and their corneas analyzed for protein, alkaline phosphatase, acid phosphatase, N-acetyl-beta-D-glucosaminidase, and lactate dehydrogenase. The enzyme activities in the inflamed corneal stroma reflect the high lysosomal activity, which probably originates from the leukocytes. The enzyme activities in the epithelium indicate that the tissue is abnormal and undergoing repair processes.
    Albrecht von Graæes Archiv für Ophthalmologie 02/1986; 224(3):288-90. · 1.93 Impact Factor
  • A Bolková, J Cejková
    Ceskoslovenská oftalmologie 04/1985; 41(2):101-6.
  • A Bolková, J Cejková
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    ABSTRACT: In order to assess the extent of injury and the response of the cornea to alkali burns, NaOH in concentrations of 0.5, 0.25, 0.1, 0.05, and 0.01 N was applied to rabbit eyes and the histologic and metabolic changes studied. The activities of alkaline and acid phosphatases in homogenates and in cold microtome sections were examined on days 1, 4, and 7 after injury. At all time intervals 0.5 N and 0.25 N NaOH induced a remarkable decrease in enzyme activities. On the other hand, after 0.05 N and 0.01 N NaOH only very slight changes were observed. Using 0.1 N NaOH, both phosphatases decreased on day 4 after treatment and acid phosphatase reached normal values in a week, whereas alkaline phosphatase increased with a maximum on day 7. Its role in synthetic processes during corneal regeneration is discussed. Both histologic and metabolic patterns in the experimentally burned cornea were shown to be a function of NaOH concentration and the duration of contact. The process of re-epithelization of the cornea during healing after 0.1 N NaOH for 1 min is described.
    Albrecht von Graæes Archiv für Ophthalmologie 02/1984; 222(2):86-9. · 1.93 Impact Factor
  • A Bolková, J Cejková
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    ABSTRACT: Alkaline and acid phosphatases were studied in the rabbit cornea following acetone and ethanol exposure to the eye. Changes in enzyme activities were investigated in homogenates of epithelium and stroma quantitatively and in frozen cryostat sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings showed a remarkable increase in alkaline phosphatase of the epithelium beginning on day 7. This activation persisted until day 28 after instillation of both noxae with maximum activity on day 14. However, acetone was proved to be significantly more effective than ethanol. In addition, a different topochemistry of alkaline phosphatase was found in the epithelium of treated corneas, i.e., enzyme activity was observed not only superficially but in all epithelial layers of the cornea as compared to a normal one. The effect of acetone and ethanol on a regenerating corneal epithelium is discussed.
    Albrecht von Graæes Archiv für Ophthalmologie 02/1983; 220(2):96-9. · 1.93 Impact Factor
  • J Cejková, A Bolková
    Ceskoslovenská oftalmologie 04/1982; 38(2):76-81.
  • A Bolková, J Cejková
    Ceskoslovenská oftalmologie 12/1979; 35(6):415-20.
  • A Bolková, J Cejková
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    ABSTRACT: Activities of alkaline and acid phosphates were investigated in rabbit corneas after a complete epithelial denudation in vivo (limbus to limbus). Dynamics of enzymatic changes during corneal healing were followed quantitatively in homogenates of regenerated epithelium and stroma (denuded cornea) and in cryostate frozen sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings at these times showed a different response in each enzyme. Acid phosphatase displayed a gradual increase of activity in epithelium as well as in stroma; on day 28 after injury its content was normal. In contrast, alkaline phosphatase showed delayed activity during the repair process, and even a month after de-epithelization was still subnormal, particularly in superficial layers of epithelium.
    Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 01/1979; 209(2):137-43.
  • A. Bolková, J. Čejková
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    ABSTRACT: Activities of alkaline and acid phosphatases were investigated in rabbit corneas after a complete epithelial denudation in vivo (limbus to limbus). Dynamics of enzymatic changes during corneal healing were followed quantitatively in homogenates of regenerated epithelium and stroma (denuded cornea) and in cryostate frozen sections on days 1, 4, 7, 14, and 28. Biochemical and histochemical findings at these times showed a different response in each enzyme. Acid phosphatase displayed a gradual increase of activity in epithelium as well as in stroma; on day 28 after injury its content was normal. In contrast, alkaline phosphatase showed delayed activity during the repair process, and even a month after de-epithelization was still subnormal, particularly in superficial layers of epithelium.Untersuchung der Aktivitten der alkalischen und sauren Phosphatasen in Kaninchenhornhuten nach totaler Abrasio in vivo. Analyse der Dynamik der Enzymnderungen whrend der Wundheilungsphase. Bericht ber die festgestellten biochemischen und histochemischen Beobachtungen. Die saure Phosphatase zeigte eine kontinuierliche Aktivittszunahme im Epithel und Stroma; Am 28. Tag nach der Abrasio war die Aktivitt der sauren Phosphatase normal. Dagegen wurde eine Aktivittsverminderung der alkalischen Phosphatase whrend der Epithelregeneration beobachtet, sogar ein Monat nach der Abrasio fiel noch eine subnormale Aktivitt, besonders in den oberflchlichen Epithelschichten auf.
    Albrecht von Graæes Archiv für Ophthalmologie 05/1978; 209(2):137-143. · 1.93 Impact Factor
  • A Bolková, J Cejková
    Ceskoslovenská oftalmologie 02/1978; 34(1):3-9.
  • J Cejková, A Bolková
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    ABSTRACT: The distribution of alkaline phosphatase was investigated in corneas of various animals by means of histochemical and biochemical methods. Special attention was paid to keratocytes that proved to be positive when a proper substrate and technique were used even if the activity differed according to animal species. Naphthol-AS-MX-phosphatate with Variamine-Blue-RT salt in the simultaneous azocoupling method was the most sensitive substrate; less suitable were naphthol-AS-phosphate, and particularly l-naphthyl-phosphate with Fast-Blue-BB-salt in the same method. Keratocytes were completely negative with beta-glycerophosphate in the Gomori technique. Contrary to keratocytes, epithelial and endothelial cells were without substrate predilection. The results of both approaches showed a wide inter-species variability. The overall activity of alkaline phosphatase detected histochemically and its activity in the unsedimentable fraction of whole corneas determined biochemically was in good agreement. The highest activity was found in the calf cornea; enzyme levels decreased gradually in the bovine, rabbit, dog, sheep, and pig cornea.
    Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 12/1977; 204(3):209-14.
  • J Cejková, A Bolková
    Ceskoslovenská oftalmologie 12/1977; 33(6):397-401.
  • A Bolková
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    ABSTRACT: The activities of acid phosphatase, beta-glucuronidase, beta-galactosidase, and N-acetyl-beta-D-glucosaminidase were investigated in the normal rabbit cornea. For all spectrophotometric assays, appropriate p-nitrophenyl derivates were used. Only beta-glucuronidase were determined employing phenolphthalein glucuronid as a substrante. Acid phosphatase revealed the highest activity, followed by N-acetyl-beta-D-glucosaminidase and beta-galactosidase. In the case of beta-glucuronidase the lowest activity was found. The results on the rabbit cornea are compared with those on some other tissues described in the literature. Correlation of biochemical data and histochemical findings in the same species is briefly discussed.
    Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 08/1977; 203(1):67-71.
  • J. Čejková, A. Bolková
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    ABSTRACT: The distribution of alkaline phosphatase was investigated in corneas of various animals by means of histochemical and biochemical methods. Special attention was paid to keratocytes that proved to be positive when a proper substrate and technique were used even if the activity differed according to animal species. Naphthol-AS-MX-phosphatate with Variamine-Blue-RT salt in the simultaneous azocoupling method was the most sensitive substrate; less suitable were naphthol-AS-phosphate, and particularly l-naphthyl-phosphate with Fast-Blue-BB-salt in the same method. Keratocytes were completely negative with -glycerophosphate in the Gomori technique. Contrary to keratocytes, epithelial and endothelial cells were without substrate predilection. The results of both approaches showed a wide interspecies variability. The overall activity of alkaline phosphatase detected histochemically and its activity in the unsedimentable fraction of whole corneas determined biochemically was in good agreement. The highest activity was found in the calf cornea; enzyme levels decreased gradually in the bovine, rabbit, dog, sheep, and pig cornea.Die Verteilung der alkalischen Phosphatase wurde in der Hornhaut von verschiedenen Species histochemisch und biochemisch bestimmt. Besondere Beachtung fanden die Keratocyten, die man gegebenenfalls nachweisen konnte, auch wenn die Fermentaktivitt verschieden war. Als empfindlichstes Substrat erwies sich Naphthol-AS-MX-Phosphat mit Variamin-Blau-RT-Salz in der simultanen Azokopulationsreaktion. Weniger geeignet waren Naphthol-AS-Phosphat, namentlich l-Naphthyl-Phosphat mit Fast-Blau-BB-Salz. Mit -Glycerophosphat in der Technik von Gomori lieen sich keine Keratocyten nachweisen. Epithel- und Endothelzellen blieben frei von den angewandten Substanzen. Insgesamt ergaben sich erhebliche Unterschiede zwischen den einzelnen Tierarten. Die Totalaktivitten der alkalischen Phosphatase waren gut korreliert. Die strkste Fermentaktivitt wurde in der Hornhaut von Klberaugen gefunden, zunehmend geringer waren die Aktivitten in der Hornhaut von Rindern, Kaninchen, Hunden, Schafen und Schweinen.
    Albrecht von Graæes Archiv für Ophthalmologie 01/1977; 204(3):209-214. · 1.93 Impact Factor
  • A. Bolkova, J. Čejková
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    ABSTRACT: Acid phosphatase, N-acetyl-β-D-glucosaminidase, β-alactosidase and β-glucuronidase were investigated in the normal corneas of several animals (rabbit, calf, bovine, dog, pig, and sheep) by means of biochemical and histochemical methods. The results of both approaches show a wide interspecies variety which are, in general, in good agreement. Pig and sheep corneas show lower activities of all enzymes investigated than those of other animals. The best correlation was found in the case of acid phosphatase which is highest in all animal species under investigation, and is most active in the epithelium as could be proved histochemically. On the other hand, acid glycosidases are more pronounced in keratocytes than in epithelial and endothelial cells. Sequence of activities of individual acid glycosidases, determined by quantitative analysis and histochemical methods, is discussed.
    Ophthalmic Research. 01/1977; 9(3):155-161.
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    ABSTRACT: In the rabbit and bovine cornea the activity of alkaline phosphatase using histochemical as well as biochemical methods was investigated. Biochemically the enzyme activity was studied in separated corneal layers. In the histochemical investigation the best results were obtained in cryostat sections using the azocoupling method with naphthol AS-MX phosphate and Variamine Blue RT Salt. The enzyme activity was found not only in the epithelium and endothelium (as was described previously) but even in keratocytes. The mutual relation of activities in the epithelium and in keratocytes differed in both species. The overall activity found by histochemical methods is in good agreement with the biochemical determination of alkaline phosphatase (p-nitrophenyl phosphate as the substrate). Besides the histochemical approach shows an uneven distribution of alkaline phosphatase activity in individual cells which cannot be assessed by the biochemical determination.
    Histochemistry 11/1976; 49(3):237-43.
  • [show abstract] [hide abstract]
    ABSTRACT: In the rabbit and bovine cornea the activity of alkaline phosphatase using histochemical as well as biochemical methods was investigated. Biochemically the enzyme activity was studied in separated corneal layers. In the histochemical investigation the best results were obtained in cryostat sections using the azocoupling method with naphthol AS-MX phosphate and Variamine Blue RT Salt. The enzyme activity was found not only in the epithelium and endothelium (as was described previously) but even in keratocytes. The mutual relation of activities in the epithelium and in keratocytes differed in both species. The overall activity found by histochemical methods is in good agreement with the biochemical determination of alkaline phosphatase (p-nitrophenyl phosphate as the substrate). Besides the histochemical approach shows an uneven distribution of alkaline phosphatase activity in individual cells which cannot be assessed by the biochemical determination.
    Histochemie 08/1976; 49(3):237-243. · 2.61 Impact Factor
  • A Bolková, J Obenberger
    Ceskoslovenská oftalmologie 08/1976; 32(4):252-7.
  • A Bolková, J Obenberger
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    ABSTRACT: Rabbit corneas were burned either with 1.0 N sodium hydroxide or 1.0 N hydrochloric acid. Enzyme activities of alkaline and acid phosphatases were examined spectrophotometrically in the homogenates of cornea, iris, aqueous humor and vitreous body. On the 3rd day after alkaline as well as acid burn, a significant decrease of both enzyme activities was produced as compared with untreated animals. A more pronounced change was found in the case of alkaline injuries. With both kinds of caustic agents the decrease of acid phosphatase activity was more striking than that of the alkaline phosphatase. Advantages and shortcomings of biochemical and histochemical enzymatic determinations in experimental ocular inflammations are briefly discussed.
    Albrecht von Graefe s Archive for Clinical and Experimental Ophthalmology 02/1976; 200(3):251-5.