ABSTRACT: The protective effects of hesperidin against hypercholesterolemia and fatty liver were examined in male Wistar rats fed a high-cholesterol diet for 12 weeks. Compared with a standard diet, a high-cholesterol diet not only increased body weights, liver weights, and serum concentration of cholesterol, but also induced the fatty degeneration (steatosis) of liver. Hesperidin (0.08%) reduced levels of hepatic steatosis, adipose tissue and liver weights (P < 0.05), serum total cholesterol and retinol binding protein (RBP) 4 concentrations (P < 0.05) in rats fed with high-cholesterol diet, while reduction in low-density lipoprotein cholesterol levels and triglyceride concentrations was not significant. It also attenuated the marked changes in mRNA expression of lipid metabolism-related proteins: RBP, heart fatty acid-binding protein (H-FABP), and cutaneous fatty acid-binding protein (C-FABP), in liver and adipose tissue. According to the results of gas chromatography, serum concentrations of total cholesterol and biomarkers of cholesterol synthesis (lathosterol) and absorption (campesterol, β-sitosterol) were lower, and concentrations of cholesterol in feces were higher in the rats given hesperidin (P < 0.05). Hesperidin may improve hypercholesterolemia and fatty liver by inhibiting both the synthesis and absorption of cholesterol and regulating the expression of mRNA for RBP, C-FABP, and H-FABP.
Journal of Pharmacological Sciences 01/2011; 117(3):129-38. · 2.08 Impact Factor
ABSTRACT: We previously reported that measuring circulating serum mRNAs using quantitative one-step real-time RT-PCR was clinically useful for detecting malignancies and determining prognosis. The aim of our study was to find crucial serum mRNA biomarkers in esophageal cancer that would provide prognostic information for post-esophagectomy patients in the critical care setting.
We measured serum mRNA levels of 11 inflammatory-related genes in 27 post-esophagectomy patients admitted to the intensive care unit (ICU). We tracked these levels chronologically, perioperatively and postoperatively, until the two-week mark, investigating their clinical and prognostic significance as compared with clinical parameters. Furthermore, we investigated whether gene expression can accurately predict clinical outcome and prognosis.
Circulating mRNAs in postoperative esophagectomy patients had gene-specific expression profiles that varied with the clinical phase of their treatment. Multivariate regression analysis showed that upregulation of IL-6, VWF and TGF-β1 mRNA in the intraoperative phase (p = 0.016, 0.0021 and 0.009) and NAMPT and MUC1 mRNA on postoperative day 3 (p < 0.01) were independent factors of mortality in the first year of follow-up. Duration of ventilator dependence (DVD) and ICU stay were independent factors of poor prognosis (p < 0.05). Therapeutic use of Sivelestat (Elaspol®, Ono Pharmaceutical Co., Ltd.) significantly correlated with MUC1 and NAMPT mRNA expression (p = 0.048 and 0.045). IL-6 mRNA correlated with hypercytokinemia and recovery from hypercytokinemia (sensitivity 80.9%) and was a significant biomarker in predicting the onset of severe inflammatory diseases.
Chronological tracking of postoperative mRNA levels of inflammatory-related genes in esophageal cancer patients may facilitate early institution of pharamacologic therapy, prediction of treatment response, and prognostication during ICU management in the perioperative period.
Journal of Translational Medicine 10/2010; 8:103. · 3.41 Impact Factor
ABSTRACT: We investigated whether a cholesterol enriched-diet upregulated mRNA expression of the lipid metabolism related-proteins, retinoid binding protein (RBP), heart fatty acid binding protein (H-FABP), and cutaneous fatty acid binding protein (C-FABP), in liver and adipose tissue, and examined the effect of ginger on the expression of these genes.
The male rats were divided into 3 groups. Control rats were fed a standard diet, the cholesterol enriched-diet group (Chs) was fed a cholesterol enriched-diet, and the cholesterol enriched-diet and ginger group (ChGs) was fed a cholesterol enriched-diet and ginger (500 mg/day) diet, respectively for 12 weeks. Each mRNA expression level was measured as the ratio of each gene relative to the beta-actin expression level, using semi-quantitative RT-PCR method.
Cholesterol enriched-diet developed hepatic steatosis with hyperlipidemia and increased RBP mRNA expression in the liver, as well as mRNA expression of RBP, H-FABP, and C-FABP in adipose tissue around the left kidney (P<0.05). This is the first report to show the upregulation of H-FABP mRNA in adipose tissue in hyperlipidemic rats. RBP mRNA was expressed in the liver on ChGs slightly lower than Chs (P=0.078).
These lipid metabolism genes are important indicators of hyperlipidemia. Ginger tends to reduce RBP mRNA expression levels in the liver and visceral fat in hyperlipidemia, and may improve lipid metabolism.
Life sciences 05/2009; 84(25-26):903-7. · 2.56 Impact Factor