Z N Chaudri

Publications (1)3.54 Total impact

• Article: Dual specificity antibodies using a double-stranded oligonucleotide bridge.

  Z N Chaudri, M Bartlet-Jones, G Panayotou, T Klonisch, I M Roitt, T Lund, P J Delves
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  ABSTRACT: The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS-PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide-linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.
  FEBS Letters 05/1999; 450(1-2):23-6. · 3.54 Impact Factor