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Publications (5)3.87 Total impact

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    ABSTRACT: Cytochrome P450 (CYP)1A1 gene polymorphism has been shown to be associated with several diseases. In this study, we evaluated the association between the polymorphism in the cytochrome P-450 (CYP)1A1 (CYP1A1) gene and genetic susceptibility to prostate cancer (PCa) in Tunisian men. One hundred and thirty eight PCa patients and the same number of controls were enrolled in this study. All DNA samples from peripheral blood leucocytes were genotyped for genetic polymorphism of the CYP1A1 gene using the polymerase chain reaction-restriction fragment length polymorphism method. The polymorphism in PCa patients was also analyzed according to age at diagnosis, tobacco use, cancer stage, and grade (Gleason score). The prevalence of CYP1A1 variants (w1m1 and m1m1) was similar in PCa patients and controls (15.22% vs. 17.39%, p=0.624 and 2.17%, respectively). No significant difference in the frequency distribution of CYP1A1 polymorphism was observed between PCa patients and controls. Furthermore, we were unable to demonstrate any significant association between the studied CYP1A1 polymorphism, age, tobacco use, and tumor parameters of aggressiveness at diagnosis.
    Genetic Testing and Molecular Biomarkers 02/2012; 16(7):661-6. · 1.44 Impact Factor
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    ABSTRACT: Prostate cancer (PCa) formation has been reported to be associated with androgen. Two key steps in the sex steroid synthesis are mediated by the enzyme cytochrome P450c 17α which is encoded in the CYP17 gene. The A2 allele of the CYP17 gene has been thought to be associated with increased functional activity of this steroidogenic enzyme. Consequently, the A2 allele has been examined as a biomarker of individual susceptibility to hormone-related diseases among men. We prospectively assessed the association between the A2 allele of CYP17 and PCa risk among 125 cases and 125 controls in a case-control study. Our aim was to investigate whether a polymorphism of CYP17 gene could be used as a genetic marker for associating PCa. The result revealed a significant association between the CYP17 polymorphic genotypes and PCa. Therefore, CYP17 gene polymorphism is likely contributed to the pathogenesis of PCa but not to disease severity.
    Cancer epidemiology. 12/2010; 35(5):480-4.
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    ABSTRACT: Several genes involved in the metabolism of carcinogenesis have been found to be polymorphic in the human population, and specific alleles are associated with increase risk of cancer of various sites. This study is focused on the polymorphic enzymes glutathione-S-transferase M1 (GSTM1) and T1 (GSTT1) that involved in the detoxification of many xenobiotics involved in the etiology of prostate cancer. To evaluate whether GSTM1 and/or GSTT1 contribute to prostate cancer (CaP) etiology, we studied 110 incident CaP cases and 122 controls. The probability of having CaP was increased in men who had homozygous deleted (non-functional) genotypes at GSTT1 (OR=2.17; 95% CI=1-3.79) but not GSTM1 (OR=0.89; 95% CI=0.66-1.88). Hence, individuals lacking the GSTT1 gene are at approximately twofold higher risk of developing prostate cancer in comparison with individuals with at least one active allele in the GSTT1 locus. These results suggest that GSTT1 is associated with CaP risk. The effect of smoking associated with the GSTT10/0 genotype was not found to affect the risk of prostate cancer.
    Cancer epidemiology. 10/2010; 34(5):598-603.
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    Journal of Genetics 05/2009; 88(1):119-22. · 0.88 Impact Factor
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    ABSTRACT: Control of opportunistic yeast is one of the factors reducing nosocomial infection in hospital. In this study, we investigated the presence of virulence factors including antifungal susceptibility, haemolytic potency, proteinase and phospholipase production. In addition biofilm formation was assessed using the XTT assay. A total of 24 Candida strains were isolated and identified. The most common species were Candida albicans and Candida glabrata (40.7%), followed by Candida dubliniensis (8.3%), Candida kefyr (4.2%) and Candida krusei (4.2%). In addition, 62.5% of strains were resistant to itraconazole and 16.7% of strains were resistant to fluconazole. All the strains were susceptible to amphotericin B. Phospholipase activity showed that 8.3% of the total strains were very strong producers and 41.7% were strong producers. Furthermore, three strains (12.5%) were found to have very strong proteinase activity. All the tested strains revealed beta haemolytic potency. All Candida strains were adhesive to polystyrene 96-well microtitre plates to differing degrees. The OD values of XTT reduction at 492nm ranged from 0.025 (ca12) to 0.927 (ca2). The majority of C. albicans strains were highly metabolically active in comparison with the other species. Control of fungal infections in hospitalised patients is important to prevent the emergence of multi-resistant strains to commonly used antifungal agents. Keywords Candida spp.-Antifungal susceptibility-Phospholipase-Proteinase-Heamolytic potency-Biofilm-XTT
    Annals of Microbiology 60(3):481-488. · 1.55 Impact Factor