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ABSTRACT: In this study, presence of dentin infection in root canals, obturated with 4 techniques submitted to the bacterial leakage test, was evaluated using histologic methods.
The canals of palatal roots of 160 molars were instrumented and divided into different groups, according to the obturation technique used (lateral condensation, MicroSeal system, Touch 'n Heat + Ultrafil, and Tagger's hybrid technique) and extent of the remaining obturation material (5 mm and 10 mm). Ten additional roots were used as control samples. The roots were sterilized in ethylene oxide and mounted on a device for evaluation of bacterial leakage using the bacteria Enterococcus faecalis for 120 days. After the leakage test, roots were microscopically analyzed for the presence of dentin infection in the root canals and dentinal tubules.
A total of 154 specimens were analyzed using both methodologies in the experimental groups; 50 root canals (32.4%) showed bacterial leakage at the end of the experimental period, and 118 (76.6%) showed the presence of bacteria in the root canals using the histologic criteria. The lateral condensation technique allowed lower penetration of bacteria in the root canals and dentinal tubules, followed by Touch 'n Heat + Ultrafil, MicroSeal, and Tagger's hybrid technique, which allowed significantly greater penetration of bacteria. Root canals with 10 mm of remaining obturation material presented similar bacterial penetration as root canals with 5 mm.
Even when an adequate seal of the apical foramen was shown by the absence of turbidity in the bacterial leakage test, E. faecalis dentin infection was present in a high percentage of the root canals after 120 days of root filling exposure to the bacteria. Tagger's hybrid technique presented greater quantity of bacteria in histologic sections than root canals obturated with the other techniques.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 05/2010; 109(5):788-94. · 1.50 Impact Factor
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ABSTRACT: This study compared the coronal bacterial leakage of root canals obturated by different techniques and with different lengths of obturation.
The canals of palatal roots of 160 maxillary molars were instrumented and divided into different groups according to the obturation technique used (lateral condensation, Microseal system, Touch 'n Heat + Ultrafil system, or Tagger's hybrid technique) and the length of obturation (5 mm or 10 mm). The roots were impermeabilized, sterilized in ethylene oxide, and mounted on a device for evaluation of the bacterial leakage.
Tagger's hybrid technique produced a statistically greater number of specimens with coronal leakage than the other techniques. There was no statistically significant difference between the lateral condensation, Touch 'n Heat + Ultrafil, and Microseal groups. Root canals with 10 mm of obturation produced a statistically significantly smaller number of specimens with leakage than root canals with 5 mm of obturation.
Tagger's hybrid technique produced a greater number of specimens with coronal leakage than the other techniques, and a greater number of root canals with 5 mm of obturation leaked than root canals with 10 mm of obturation.
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 02/2008; 105(1):e48-53. · 1.50 Impact Factor
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ABSTRACT: The purpose of this study was to compare the apical sealing of root canals obturated with different techniques. One hundred-six human mandibular incisors were submitted to instrumentation by means of the step-back technique. After instrumentation, one hundred teeth received an impermeable coating on the external surfaces of the crown and root (except for the area nearby the apical foramen). Afterwards, they were divided in five groups containing twenty elements each, according to the obturation technique employed: 1. lateral condensation with Kerr file; 2. continuous wave of condensation technique with System B; 3. thermoplasticized injectable gutta-percha technique with the Ultrafil system; 4. mechanically thermoplasticized gutta-percha with the JS Quick-Fill system and 5. thermoplasticized gutta-percha associated to a master cone with the Microseal system. The six remaining teeth were employed as negative and positive controls. After obturation, the access cavities were sealed and the teeth were immersed in aqueous 2% methylene blue dye for 72 hours at 37ºC. After that, the teeth were longitudinally sectioned and the apical microleakage was evaluated in a stereomicroscope. The Microseal system presented the best apical sealing ability, followed by System B, JS Quick-Fill, Ultrafil and the lateral condensation technique. The statistical analysis of the results demonstrated that: 1. the Microseal system presented an apical sealing similar to System B and better than the other groups; 2. System B presented better apical sealing than the lateral condensation technique, being similar to the other groups; and 3. the lateral condensation, Ultrafil and JS Quick-Fill groups demonstrated similar sealing ability.
Journal of Applied Oral Science 09/2003; 11(3):181-5. · 0.57 Impact Factor
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ABSTRACT: Root canal preparation has been considered one of the most important steps in root canal therapy, thus many techniques and instruments have been developed. The aim of this study was to evaluate the cleaning of the root canal through three different instrumentation techniques. Thirty mandibular incisors were selected and submitted to lingual access cavities. Afterwards, the canals were filled with India ink dye previously stored in carpules, which was inserted into the root canal by means of anesthetic syringe and anesthetic needles. After 48 hours, during which the dye was allowed to dry inside the root canal, the teeth were divided in three groups: G1- GT rotatory instrumentation; G2- manual instrumentation; G3- association of both. After instrumentation, the teeth were longitudinally sectioned. The cleaning process accomplished through the different instrumentation techniques was evidenced by dye removal at the cervical, middle and apical thirds of the root canal. The results of this study showed that were not statistically significant differences between these three instrumentation techniques for all three thirds of the root canal.
Journal of Applied Oral Science 03/2003; 11(1):35-9. · 0.57 Impact Factor
