Teng-fei Xu

Qingdao University, Qingdao, Shandong Sheng, China

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Publications (2)0 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To construct a shuttle plasmid encoding a chimeric gene of Clostridium saccharobutylicum eglA promoter(eglA p)-extracellular domain of human epidermal growth factor receptors 2(hHer2/neu ECD)-human Interleukin-12(rhIL-12), pIMP1 eglA p-hHer 2/neu ECD-rhIL-12. The hHer2/neu ECD and the eglA p was amplified from the corresponding template: pcDNA3.1 hHer2/neu and 55 bp fragment in eglA p by PCR. pcDNA6 eglA p-hHer2/neu ECD-rhIL-12 was prepared by inserting the hHer2/neu ECD and eglAp fragment into the plasmid, pcDNA6 rhIL-12. Shuttle plasmid pIMP1 eglA p-hHer2/neu ECD-rhIL-12 was acquired by using in-fusion technique. subsequently, the recombinant shuttle plasmid was identified. All the fragments of hHer2/neu ECD , eglA p and eglAp-Her2/neu ECD-rhIL-12 were amplified, and the corresponding plasmids were prepared rightly. The shuttle plasmid of pIMP1 eglAp-hHer2/neu ECD-rhIL-12 was successfully const- ructed. No error was found both in the sequence and ORF of the acquired chimeric gene. A shuttle plasmid encoding a chimeric gene of Clostridium saccharobut- ylicum eglAp-hHer2/neu ECD-rhIL-12(pIMP1 eglA p-hHer2/neu ECD-rhIL- 12) was success- fully constructed.
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 04/2011; 27(4):370-3.
  • Teng-fei Xu, Wen-qing Zhang, Hong Yu, Dan Li
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    ABSTRACT: To construct an eukaryotic vector encoding extracellular domain of human epidermal growth factor receptors (HER2), pcDNA6/v5-his-HER2, and to screen HER2 positive clones from mouse breast cancer cell line EMT6. The extracellular domain of HER2 was amplified from pcDNA3.1-HER2 by PCR. pcDNA6/v5-his-HER2 was prepared by inserting the fragment into the plasmid pcDNA6/v5-his. Then the recombinant vector was identified by restriction enzyme and sequencing. Next, pcDNA6/v5-his-HER2 was transfected into the EMT6 cell line and the positive clones (EMT6/HER2) were screened with blasticidin. Finally, the expression of HER2 in EMT6/HER2 was detected by RT-PCR and immunohistochemistry. The fragment of HER2 was amplified and pcDNA6/v5-his-HER2 was prepared successfully. No errors were found both in the sequence and ORF of the acquired fragment. The expected fragment of HER2 (1896 bp) was amplified from EMT6/HER2 by RT-PCR and positive signals of HER2 were detected in EMT6/HER2 by immunohistochemistry. An eukaryotic plasmid encoding HER2 (pcDNA6/v5-his-HER2) has been constructed and a cell line expressing HER2 stably has been prepared successfully.
    Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 04/2009; 25(3):226-8.