[Show abstract][Hide abstract] ABSTRACT: Results of a first-stage Sea Urchin Genome Project are summarized here. The species chosen was Strongylocentrotus purpuratus, a research model of major importance in developmental and molecular biology. A virtual map of the genome was constructed
by sequencing the ends of 76,020 bacterial artificial chromosome (BAC) recombinants (average length, 125 kb). The BAC-end
sequence tag connectors (STCs) occur an average of 10 kb apart, and, together with restriction digest patterns recorded for
the same BAC clones, they provide immediate access to contigs of several hundred kilobases surrounding any gene of interest.
The STCs survey >5% of the genome and provide the estimate that this genome contains ≈27,350 protein-coding genes. The frequency
distribution and canonical sequences of all middle and highly repetitive sequence families in the genome were obtained from
the STCs as well. The 500-kb Hox gene complex of this species is being sequenced in its entirety. In addition, arrayed cDNA libraries of >105 clones each were constructed from every major stage of embryogenesis, several individual cell types, and adult tissues and
are available to the community. The accumulated STC data and an expanding expressed sequence tag database (at present including
>12,000 sequences) have been reported to GenBank and are accessible on public web sites.
Proceedings of the National Academy of Sciences 08/2000; 97(17):9514-9518. DOI:10.1073/pnas.160261897 · 9.67 Impact Factor