Carson D Baldwin,
Gerald B Howe,
Ranga Sampath,
Larry B Blyn,
Heather Matthews,
Vanessa Harpin,
Thomas A Hall,
Jared J Drader, Steve A Hofstadler,
Mark W Eshoo,
Karl Rudnick,
Karen Studarus,
David Moore,
Sharon Abbott,
J Michael Janda,
Chris A Whitehouse
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ABSTRACT: Polymerase chain reaction electrospray ionization mass spectrometry (PCR/ESI-MS) was tested for its ability to accurately identify a blinded panel of 156 diverse bacterial isolates, mostly human and/or animal pathogens. Here, 142/156 (91%) isolates were correctly identified to the genus level and 115/156 (74%) were correctly identified to the species level. Only 9% were misidentified. This study shows that multilocus PCR/ESI-MS has the potential to be a useful technique for identifying a broad range of bacteria.
Diagnostic microbiology and infectious disease 03/2009; 63(4):403-8. · 2.45 Impact Factor