Somjai Wongtripop

National Center for Genetic Engineering and Biotechnology (BIOTEC), Bang Kadi, Pathum Thani, Thailand

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Publications (5)10.88 Total impact

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    ABSTRACT: Sand polychaetes (Perinereis nuntia) have been used as a live feed to boost reproductive maturation before mating in hatchery farms in Thailand. However, no study has evaluated the effect of polychaetes on performance and physiology of the sperm of the domesticated black tiger shrimp (Penaues monodon). Therefore, this study aims to study the effects of sand polychaetes on growth, survival, reproductive performance and sperm physiology and morphology of domesticated male broodstock P. monodon. After feeding with either polychaetes or commercial pellets for four weeks, growth and survival rates of polychaete-fed group were significantly higher than those of pellet-fed group. Spermatophore weight and total sperm counts of the polychaete-fed group were significantly higher than those of the pellet-fed group at Weeks 3 and 4, whereas % abnormal sperm cells and % acrosome reaction of polychaete-fed group were significantly lower and higher than those of pellet-fed group only at Week 4, respectively. Physiology of spermatophores in the polychaete-fed group was white and opaque while that of the pellet-fed group was pigmented. Morphological changes of sperm revealed less abnormal sperms (misshaped half, misshaped head, and misshaped tails) in the polychaete-fed group. Nutritional analysis revealed that polychaetes have significantly higher total protein, fat contents and essential fatty acids (arachidonic and eicosapentaenoic acids) but significantly lower fiber content. Moreover, histological analysis of hepatopancreas revealed more vacuoles, which are storages for glycogen and lipids in the polychaete-fed group than in the pellet-fed group. Therefore, this study provides an evidence that polychaetes provide benefits to growth, survival and sperm performance for boosting reproductive maturation in male domesticated broodstock Penaeus monodon.
    Aquaculture. 01/2014; 433:266–275.
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    ABSTRACT: We exploited Artemia as a double-stranded (ds)RNA-delivery system to combat viral diseases in shrimp. First, the transformed Escherichia coli (E.coli) expressing red fluorescent protein (RFP) was tested in the Artemia enrichment process. RFP signals detectable in the gut of Artemia under confocal microscope were evident for the successful encapsulation. Second, the Artemia enrichment process was performed using E.coli producing Laem-Singh virus (LSNV)-specific dsRNA, which has been previously shown to inhibit the viral infection in the black tiger shrimp Penaeus monodon by intramuscular injection and oral administration. The enriched Artemia nauplii were confirmed to contain dsRNA-LSNV by RT-PCR, and were subjected to the feeding test with P.monodon postlarvae. Quantitative RT-PCR indicated that a number of LSNV copies in most of the treated shrimp were, at least, 1000-fold lower than the untreated controls. During 11-17 weeks after feeding, average body weight of the treated group was markedly increased relative to the control group. A smaller differential growth rate of the treated group as compared to the control was also noticed. These results suggested that feeding shrimp with the dsRNA-enriched Artemia can eliminate LSNV infection, which is the cause of retarded growth in P.monodon. The present study reveals for the first time the therapeutic effect of dsRNA-enriched Artemia for shrimp disease control.
    Antiviral research 08/2013; · 3.61 Impact Factor
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    ABSTRACT: Viral inhibition by double-stranded (ds)RNA is a potential therapeutic approach for controlling shrimp viral diseases. Here, we describe the successful oral application of dsRNA targeting Laem-Singh Virus (LSNV) to diminish monodon slow growth syndrome (MSGS) in Thai Penaeus monodon. Shrimp feed formulated with bacterially expressed LSNV-dsRNA was given to shrimp for 9 weeks. RT-PCR results revealed that all control shrimp were LSNV-positive at the end of experiment, while the shrimp that received dsRNA-feed exhibited 20-60% LSNV reduction. The average body weight of treated shrimp (number of shrimp=100) was significantly higher than that of the control group. Such increase is likely due to the elimination of MSGS caused by LSNV, as size variation of the treated group is much lower than that in the control group. This study demonstrates for the first time that feed with LSNV-specific dsRNA promotes the overall growth of P. monodon and relieves MSGS condition in LSNV-infected shrimp. The work reaffirms the potential of dsRNA application for controlling viral disease in shrimp farming.
    Journal of Biotechnology 02/2013; · 3.18 Impact Factor
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    ABSTRACT: Marine invertebrates respond to fluctuations in their environment throughout development and growth. Homeostatic adaptation is mediated in part through the regulation of Na+‐K+ ATPase enzymes and members of this class of enzymes are localized to the antennal gland of many crustaceans. In this study, we investigated the morphological and biochemical changes of the antennal glands in eight families of cross‐breed post‐larval shrimp subjected to hypotonic (10 ppt) and isotonic (20 ppt) conditions. The greatest changes in the antennal glands were detected in the hypotonic condition with families two and five cross‐breed shrimp exhibiting the highest tolerance to this diluted salinity. The changes detected in hypotonic tolerant shrimp included enlargement of coelomosac and labyrinth spaces, the presence of many dense cytoplasmic globules in podocytes as well as highly wavy apical architecture of labyrinth cells. Several alterations were also observed at the subcellular level such as widening of the intercellular spaces between podocytes, organelle destruction, and decreased basal membrane in‐folding in labyrinth cells. In addition, shrimp exposed to hypotonic conditions displayed decreased expression of both α and β subunits of Na+‐K+ ATPase and decreased enzymatic activity. The changes observed in animals upon exposure to hypotonic stress are discussed.
    Aquaculture Research 01/2013; 44(7). · 1.42 Impact Factor
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    ABSTRACT: A new microsporidian species, Enterocytozoon hepatopenaei sp. nov., is described from the hepatopancreas of the black tiger shrimp Penaeus monodon (Crustacea: Decapoda). Different stages of the parasite are described, from early sporogonal plasmodia to mature spores in the cytoplasm of host-cells. The multinucleate sporogonal plasmodia existed in direct contact with the host-cell cytoplasm and contained numerous small blebs at the surface. Binary fission of the plasmodial nuclei occurred during early plasmodial development and numerous pre-sporoblasts were formed within the plasmodium. Electron-dense disks and precursors of the polar tubule developed in the cytoplasm of the plasmodium prior to budding of early sporoblasts from the plasmodial surface. Mature spores were oval, measuring 0.7x1.1microm and contained a single nucleus, 5-6 coils of the polar filament, a posterior vacuole, an anchoring disk attached to the polar filament, and a thick electron-dense wall. The wall was composed of a plasmalemma, an electron-lucent endospore (10nm) and an electron-dense exospore (2nm). DNA primers designed from microsporidian SSU rRNA were used to amplify an 848bp product from the parasite genome (GenBank FJ496356). The sequenced product had 84% identity to the matching region of SSU rRNA from Enterocytozoon bieneusi. Based upon ultrastructural features unique to the family Enterocytozoonidae, cytoplasmic location of the plasmodia and SSU rRNA sequence identity 16% different from E. bieneusi, the parasite was considered to be a new species, E. hepatopenaei, within the genus Enterocytozoon.
    Journal of Invertebrate Pathology 07/2009; 102(1):21-9. · 2.67 Impact Factor