Mami Hosokawa, Ritsuko Kadota,
Shigeki Shichijo,
Kyogo Itoh,
Igor Dmitriev,
Victor Krasnykh,
David T Curiel,
Yoichi Takue,
Hiro Wakasugi,
Shigemitsu Takashima,
Yuji Heike
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ABSTRACT: The biological function of the SART-1 gene product is demonstrated and its potential as a target for cancer gene therapy is discussed.
The SART-1 gene was transduced by a recombinant adenovirus vector and its expression was promoted by a CMV promoter.
The transduction efficiency by recombinant adenoviruses in A549 and MCF-7 cells was determined using a vector expressing luciferase, which showed high expression in the cells. Cell count analysis using Trypan-Blue dye exclusion showed that SART-1 gene transduction inhibited cell growth. Flow cytometry analysis suggested that SART-1 gene transduction induced cell cycle arrest followed by apoptosis. Western blot analysis confirmed that the apoptosis pathway was activated by SART-1 gene transduction.
These results show that SART-1 gene transduction induces cell cycle arrest leading to apoptosis and suggest the possibility of gene therapy against cancer. In addition, SART-1 is known to be a tumor antigen in a range of cancers recognized by T cells, thus a potential strategy would be the combination of suicide gene therapy with immuno-gene therapy.
Anticancer research 25(3B):1983-90. · 1.73 Impact Factor
