Ratanakanit Harnprasopwat

Publications (3)4.82 Total impact

• Article: miRNAs in normal and malignant B cells.

  Ai Kotani, Ratanakanit Harnprasopwat, Takae Toyoshima, Toyotaka Kawamata, Arinobu Tojo
  [show abstract] [hide abstract]
  ABSTRACT: MicroRNAs (miRNA) are small noncoding RNAs that posttranscriptionally regulate expression of target mRNAs. In animals, miRNAs control many developmental and physiological processes. It has recently revealed that miRNAs critically work in B cell development and some B cell malignancy and that their expression profiles well correlate with its prognosis and phenotype. This review overviews these interesting findings and discuss a possibility that miRNA is a promising target for therapeutic and diagnostic tool for B cell malignancy.
  International journal of hematology 09/2010; 92(2):255-61. · 1.17 Impact Factor
• Article: Alteration of processing induced by a single nucleotide polymorphism in pri-miR-126.

  Ratanakanit Harnprasopwat, Daon Ha, Takae Toyoshima, Harvey Lodish, Arinobu Tojo, Ai Kotani
  [show abstract] [hide abstract]
  ABSTRACT: MicroRNAs (miRNAs) are small non-coding RNAs that inhibit expression of specific target genes at the post-transcriptional level. Sequence variations in miRNA genes, including pri-miRNAs, pre-miRNAs and mature miRNAs, could potentially influence the processing and/or target selection of miRNAs. In this study, we have found the single nucleotide polymorphism (SNP) at the twenty-fourth nucleotide (+24) of the mature miR-126 in the genome of RS4;11 cells, derived from a MLL-AF4 ALL patient. Through a series of in vivo analyzes, we found that this miR-126 SNP significantly blocks the processing of pri-miRNA to mature miRNA, as well as reduces miRNA-mediated translational suppression. Moreover, its frequency is different among races. Thus, our study emphasizes the importance of identifying new miRNA SNP and its contribution to miRNA biogenesis which is possible link to human genetic disease.
  Biochemical and Biophysical Research Communications 08/2010; 399(2):117-22. · 2.48 Impact Factor
• Article: Comparison of mesenchymal stem cells derived from arterial, venous, and Wharton's jelly explants of human umbilical cord.

  Ikuo Ishige, Tokiko Nagamura-Inoue, Masaki J Honda, Ratanakanit Harnprasopwat, Michiko Kido, Mitsuhiro Sugimoto, Hiromitsu Nakauchi, Arinobu Tojo
  [show abstract] [hide abstract]
  ABSTRACT: We isolated mesenchymal stem cells (MSC) from arteries (UCA), veins (UCV), and Wharton's jelly (UCWJ) of human umbilical cords (UC) and determined their relative capacities for sustained proliferation and multilineage differentiation. Individual UC components were dissected, diced into 1-2 mm(3) fragments, and aligned in explant cultures from which migrating cells were isolated using trypsinization. Preparations from 13 UCs produced 13 UCWJ, 11 UCV, and 10 UCA cultures of fibroblast-like, spindle-shaped cells negative for CD31, CD34, CD45, CD271, and HLA-class II, but positive for CD13, CD29, CD44, CD73, CD90, CD105, and HLA-class I. UCV cells exhibited a significantly higher frequency of colony-forming units fibroblasts than did UCWJ and UCA cells. Individual MSCs could be selectively differentiated into osteoblasts, chondrocytes, and adipocytes. When compared for osteogenic potential, UCWJ cells were the least effective precursors, whereas UCA-derived cells developed alkaline phosphatase activity with or without an osteogenic stimulus. UC components, especially blood vessels, could provide a promising source of MSCs with important clinical applications.
  International journal of hematology 09/2009; 90(2):261-9. · 1.17 Impact Factor