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ABSTRACT: The comet assay or single-cell gel electrophoresis (SCGE) assay is now widely accepted as a standard method for assessing
DNA damage in individual cells. It finds use in a broad variety of applications including human biomonitoring, genotoxicology,
ecological monitoring and as a tool for investigation of DNA damage and repair in different cell types in response to a range
of DNA-damaging agents. The comet assay should be eminently suitable for use in clinical practice since it is a relatively
simple and inexpensive technique which requires only a few cells, and results can be obtained within a matter of hours. This
method can be used in the study of cancer as well as in lifestyle and dietary studies. In cancer it is useful for measuring
DNA damage before, throughout and after therapy (either radiotherapy or chemotherapy). Another use of this method is in lifestyle
study, such as investigation of the effect on DNA of common human activities (e.g. smoking, or working with a potentially
genotoxic agent). The final use of comet assay in this paper is dietary study. In this type of study we observe the effects
of consumption of specific foods or supplements which may be protective for DNA against damage.
Der "Comet-Assay" oder Einzelzell Gel Elektrophorese Assay wird heute allgemein als Standard Methode zur Bestimmung von DNS-Schäden
in individuellen Zellen anerkannt. Er findet seine Verwendung in einer großen Variation von Anwendungen, wie zum Beispiel
dem humanen Bio-Monitoring, der Gentoxikologie, dem ökologischem Monitoring oder als Werkzeug zur Erforschung von Schäden
und Reparatur der DNS verschiedener Zelltypen als Antwort auf eine Vielzahl von DNS schädigenden Stoffen. Der "Comet-Assay"
sollte besonders geeignet für die klinische Praxis-Sein, da es sich um einen relativ billigen Test mit relativ einfacher Technik
handelt, der nur wenige Zellen benötigt. Die Ergebnisse liegen innerhalb von Stunden vor. Die Methode kann sowohl bei Krebs-Studien
als auch bei Lifestyle- und Diät-Studien angewandt werden: Bei Krebs-Studien ist sie zur Erfassung der DNS-Schäden vor, während
und nach einer Therapie (entweder Bestrahlung oder Chemotherapie) nützlich. Bei Lifestyle-Studien kann sie zur Erforschung
der Wirkung von häufigen humanen Aktivitäten (zum Beispiel Rauchen oder Arbeiten mit einem potentiell gentoxischem Stoff)
auf die DNS angewandt werden. Schließlich kann der "Comet-Assay" auch die Wirkung von verschiedenen Diäten auf die DNS erfassen.
In einer solchen Studie beobachten wir die Wirkung vom Verzehr von spezifischen Nahrungs- und Ergänzungsmittel, die eine DNS-schützende
Wirkung haben können.
KeywordsComet assay–DNA damage–Cancer–Nutrition
Wiener klinische Wochenschrift 04/2012; 123(23):693-699. · 0.81 Impact Factor
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[show abstract]
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ABSTRACT: The comet assay or single-cell gel electrophoresis (SCGE) assay is now widely accepted as a standard method for assessing DNA damage in individual cells. It finds use in a broad variety of applications including human biomonitoring, genotoxicology, ecological monitoring and as a tool for investigation of DNA damage and repair in different cell types in response to a range of DNA-damaging agents. The comet assay should be eminently suitable for use in clinical practice since it is a relatively simple and inexpensive technique which requires only a few cells, and results can be obtained within a matter of hours. This method can be used in the study of cancer as well as in lifestyle and dietary studies. In cancer it is useful for measuring DNA damage before, throughout and after therapy (either radiotherapy or chemotherapy). Another use of this method is in lifestyle study, such as investigation of the effect on DNA of common human activities (e.g. smoking, or working with a potentially genotoxic agent). The final use of comet assay in this paper is dietary study. In this type of study we observe the effects of consumption of specific foods or supplements which may be protective for DNA against damage.
Wiener klinische Wochenschrift 12/2011; 123(23-24):693-9. · 0.81 Impact Factor
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ABSTRACT: There has been growing interest in the quantitative determination of biochemical predictors of atherogenesis. The aim of the present study was to investigate association of lipoperoxidation biomarkers known to be pro-atherogenic (thiobarbituric acid reactive substance activity, TBARS) or anti-atherogenic (alpha-tocopherol) with the fatty acid status, and relate it to the coronary artery disease (CAD) as assessed by coronary angiography in patients with stable angina pectoris. We found that serum lipoproteins and TBARS did not differ significantly. However there was significant correlation of TBARS with total vitamin E (P=0.02) and vitamin E in very low-density lipoprotein (VLDL) (P=0.02) and low-density lipoprotein (LDL) (P=0.01), with LDL-linoleic acid (P=0.01), and high-density lipoprotein-linoleic acid (P=0.02). There was significant correlation of total vitamin E (P=0.01) and VLDL-vitamin E (P=0.01) with the degree of CAD. We conclude that TBARS and alpha-tocopherol could not be evaluated as biomarkers for the severity of CAD among the patients with stable angina pectoris.
Talanta 07/2003; 60(2-3):505-513. · 3.79 Impact Factor
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ABSTRACT: There has been growing interest in the quantitative determination of biochemical predictors of atherogenesis. The aim of the present study was to investigate association of lipoperoxidation biomarkers known to be pro-atherogenic (thiobarbituric acid reactive substance activity, TBARS) or anti-atherogenic (α-tocopherol) with the fatty acid status, and relate it to the coronary artery disease (CAD) as assessed by coronary angiography in patients with stable angina pectoris. We found that serum lipoproteins and TBARS did not differ significantly. However there was significant correlation of TBARS with total vitamin E (P=0.02) and vitamin E in very low-density lipoprotein (VLDL) (P=0.02) and low-density lipoprotein (LDL) (P=0.01), with LDL-linoleic acid (P=0.01), and high-density lipoprotein-linoleic acid (P=0.02). There was significant correlation of total vitamin E (P=0.01) and VLDL-vitamin E (P=0.01) with the degree of CAD. We conclude that TBARS and α-tocopherol could not be evaluated as biomarkers for the severity of CAD among the patients with stable angina pectoris.
Talanta.
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ABSTRACT: An increased risk of cardiovascular morbidity and mortality in diabetes mellitus type 2 has been associated with disturbances of lipid homeostasis. Recently, decreased intestinal absorption of cholesterol and increased liver cholesterol production have been reported. To investigate the influence of cholesterol lowering therapy using statin on cholesterol turnover in diabetes mellitus type 2, the levels of non-cholesterol based sterols were studied.One hundred and thirty five patients with type 2 diabetes and non-diabetic controls with cardiovascular diseases were studied. Both groups were divided into two subgroups: treated with atorvastatin and without statin therapy. The diabetics showed significantly higher levels of lathosterol (6.97 μmol l−1 versus 5.11 μmol l−1, p = 0.012) and lower levels of sitosterol (5.03 μmol l−1 versus 8.98 μmol l−1, p < 0.001) and campesterol (6.35 μmol l−1 versus 9.80 μmol l−1, p < 0.001). Non-diabetics showed no significant differences in non-cholesterol based sterols in relation to atorvastatin therapy. A significantly lower level of lathosterol as well as a decrease in lathosterol/cholesterol ratio in the statin treated groups was found in diabetics (4.11 μmol l−1 versus 7.83 μmol l−1, p < 0.001). The results based on ANOVA analysis show that the effect of atorvastatin on the lathosterol level is more pronounced in diabetics. Regression analysis showed the relationship between increased triglycerides levels and the increase in cholesterol synthesis. The calculated regression model for log lathosterol in diabetics has the following form: log(lathosterol) = 2.76 − 0.52·statin + 0.22·cholesterol (ANOVA, p < 0.001, R2 = 34%, p = 0.005 for statin, p < 0.001 for cholesterol).We conclude that in spite the total cholesterol level in diabetics type 2 is not increased, its endogenous synthesis is enhanced. Our results show that the diabetics type 2 with increased serum lathosterol and expressed metabolic syndrome (mild increase of triglycerides) might represent a suitable group for intensive treatment with statins.
Pharmacological Research.
