R M Barretto Cicarelli

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Publications (1)1.13 Total impact

  • Article: Preparation of N2, N2,7-trimethylguanosine affinity columns.
    R Espuny, D Bahia, R M Barretto Cicarelli, C Codony, A Khaouja, A M Aviñó, R Eritja, M Bach-Elias
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    ABSTRACT: 2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG-binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.
    Nucleosides & nucleotides 02/1999; 18(1):125-36. · 1.13 Impact Factor

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