Publications (2)0 Total impact
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Article: [Functional analysis of SNPs in porcine TLR4 gene].
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ABSTRACT: Toll-like receptor 4 (TLR4) plays an important role in immune response and the polymorphism in it might affect protein signaling and host resistance/susceptibility to disease. This study was designed to characterize the functional relevance of 3 nonsynonymous single nucleotide polymorphisms (SNPs), c.611 T>A (p.Leu204His), c.1027 C>A (p.Gln343Lys), and c.1605 G>T (p.Leu535Phe), which were selected based on our previous studies. RT-PCR method was used to clone the complete coding sequence of porcine TLR4 gene and the PCR-based method was used to introduce the point mutation. The effects of 3 SNPs on the ligand recognition and signaling of porcine TLR4 were investigated in transiently transfected PK-15 cells using dual-luciferase reporter system and Western blotting method. At the same time, the distribution of c.1605 G>T among pig populations composed of Min pig, Yorkshire, Landrace, and Wild boar from northeastern China was studied by created restriction site PCR-RFLP method. The complete coding sequence of TLR4 gene in Min pig and 3 variants with single point mutations were obtained. Eukaryotic expression vectors containing different alleles of porcine TLR4 were constructed. SNP c.1605 G>T significantly decreased the TLR4 signaling (P<0.01) and the polymorphism only existed in Min pig and Wild boar from northeastern China with high frequencies. SNP c.1605 G>T in porcine TLR4 might affect the receptor function and host resistance/susceptibility to diseases.Hereditas (Beijing) 08/2012; 34(8):1050-6. -
Article: [Analysis of SNPs in partial exons and 3'UTR of CAPN1 in porcine].
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ABSTRACT: In this study, Wild boar, Min pig and Yorkshire were used to investigate the effect of CAPN1 gene on lean meat percentage and tenderness. Seven pairs of primers for exons and 3'untranslated region of CAPN1 gene were designed according to pig sequences from GenBank. Polymorphisms within exons were detected by PCR-SSCP method. SNPs in 3'untranslated region were detected by DNA sequencing, and PCR-RFLP method was then developed to screen the population. Totally, 11 SNPs, of which 5 from exons including one missense mutation resulting in amino acid substitution of M/V at the position of 260, 4 from introns and 2 from 3'untranslated region, were identified. Population genetics analysis showed that the distribution of genotypes among Yorkshire, Min pig and Wild boar were extremely significant difference (P<0.01), while there were no significant difference between Min pig and Wild boar (P>0.05), and extremely significant difference existed between Min pig and Yorkshire (P<0.01). Association of polymorphisms with breed characteristics indicated that the mutations detected by P4, P6 primers and that resulting in the change of Hinf 1 recognition site may be related to lean meat percentage.Hereditas (Beijing) 06/2008; 30(6):741-6.
