ABSTRACT: The aim of this study was to verify the capacity of IBDV strains, Lukert, F52/70 and very virulent Brazilian genotype (G11) to infect and replicate on cell culture maintained without serum or protein supplementation. BHK21 and CER cells were directed adapted to growth under absence of protein and/or fetal bovine serum with addition of 5 g mL 1 of insulin-like growth factor I (IGF-I). The RPMI 1640 medium plus IGF-I was able to support both cell lines growth at a maximum of 3,3106 and 3,4106 cells mL 1 for CER and BHK21, respectively. The comparison between RPMI 1640 medium plus IGF-I and conventional MEM plus fetal bovine serum (FBS) growth curves demonstrated no significant difference, however the lowest surveillance of BHK21 and CER cells under MEM plus BFS growth Both cells provided infectious virus titres of up to 105.5 50% tissue culture infective doses 100 L 1 (TCID50 100 L 1). Furthermore, they prove to be susceptible to infection with three different IBDV strains by culture both cells with RPMI 1640 medium plus IGF-I medium. It was concluded that both cells may be used for laboratory multiplication of IBDV strains without any serum and/or animal protein with a considerable ethical impact.
American Journal of Agricultural and Biological Science. 01/2008;