Nataliya Makogon

Publications (2)3.84 Total impact

• Source

  Available from: pan.olsztyn.pl

  Article: Poly(ADP-ribose) polymerase inhibitor, 3-aminobenzamide, protects against experimental immune ovarian failure in mice.

  Nataliya Makogon, Tatyana Voznesenskaya, Tatyana Bryzgina, Vera Sukhina, Nataliya Grushka, Irina Alexeyeva
  [show abstract] [hide abstract]
  ABSTRACT: The activation of the nuclear enzyme poly(ADP-ribose) polymerase (PARP) may play an important role in numerous pathological conditions. The aim of the present study was to clarify the role of PARP in the pathogenesis of immune ovarian failure in mice and to examine the possible protective action of PARP inhibitor, 3-aminobenzamide (3-ABA). An experimental ovarian injury induced in mice by immunization with allogenic ovarian extract impaired the meiotic maturation of oocytes and increased apoptosis and necrosis of follicular cells and cells isolated from the spleen, lymph nodes and thymus. The immunization affected blood leukogram indicating the presence of an inflammatory response. Treatment with 3-ABA (1 h before antigen administrations, 0.02 mg/g intraperitoneally, twice a week during the three-week experiment) was effective to prevent meiotic maturation impairment, cell death and inflammation. The decrease in the necrosis of follicular and immune cells after 3-ABA treatment was more pronounced than that in apoptosis. It is concluded that PARP may contribute to immune-mediated ovary injury and PARP inhibitor, 3-ABA, protects against experimental immune ovarian failure, partially via a decrease in necrotic cell death.
  Reproductive biology 11/2010; 10(3):215-26. · 1.92 Impact Factor
• Source

  Available from: pan.olsztyn.pl

  Article: Melatonin protects against experimental immune ovarian failure in mice.

  Tatyana Voznesenskaya, Nataliya Makogon, Tatyana Bryzgina, Vera Sukhina, Nataliya Grushka, Irina Alexeyeva
  [show abstract] [hide abstract]
  ABSTRACT: Experimental immune ovarian failure induced in CBA mice by either administration of xenogenic anti-ovarian antibodies or immunization with allogenic ovarian extracts impaired the meiotic maturation of oocytes and increased apoptosis of follicular cells. Immunization was accompanied with the inflammation and active immune reaction, as shown by the enlargement of regional lymph nodes, the increase of apoptosis in cultured lymph node cells and the increase of band and segmented neutrophil percentage in the blood. Triple injections of melatonin (5 mg/kg of the body weight) an hour before antibodies administration restored the meiotic maturation of oocytes and supported the survival of follicular and lymph node cells. In contrast, melatonin application upon immunization was not effective to prevent the ovary impairment and cell death. It is concluded that melatonin protects against immune ovary failure induced by xenogenic anti-ovarian antibodies.
  Reproductive biology 12/2007; 7(3):207-20. · 1.92 Impact Factor