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Jing Sun,
Michael D Masterman-Smith,
Nicholas A Graham,
Jing Jiao,
Jack Mottahedeh,
Dan R Laks,
Minori Ohashi,
Jason DeJesus,
Ken-ichiro Kamei,
Ki-Bum Lee, [......],
R Michael van Dam,
Michael E Phelps,
Hong Wu,
Linda M Liau,
Paul S Mischel,
Jorge A Lazareff,
Harley I Kornblum,
William H Yong,
Thomas G Graeber,
Hsian-Rong Tseng
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Jing Sun,
Michael D Masterman-Smith,
Nicholas A Graham,
Jing Jiao,
Jack Mottahedeh,
Dan R Laks,
Minori Ohashi,
Jason DeJesus,
Ken-ichiro Kamei,
Ki-Bum Lee, [......],
R Michael van Dam,
Michael E Phelps,
Hong Wu,
Linda M Liau,
Paul S Mischel,
Jorge A Lazareff,
Harley I Kornblum,
William H Yong,
Thomas G Graeber,
Hsian-Rong Tseng
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Nangang Zhang,
Yuliang Deng,
Qidong Tai,
Boran Cheng,
Libo Zhao,
Qinglin Shen,
Rongxiang He,
Longye Hong,
Wei Liu,
Shishang Guo,
Kan Liu,
Hisan-Rong Tseng,
Bin Xiong,
Xing-Zhong Zhao
[show abstract]
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ABSTRACT: A nanostructured platform combining an electrospun TiO(2) nanofiber-deposited substrate and a cell-capture agent is created by S. S. Guo, K. Liu, H.-R. Tseng, B. Xiong, X.-Z. Zhao, and co-workers. The platform, described on page 2756, realizes the capture of significant amounts of circulating tumor cells. The enhanced local topographic interactions between the horizontally packed TiO(2) nanofiber-deposited substrates and the extracellular matrix scaffolds, in addition to anti-EpCAM/EpCAM biological recognition, contributes to the significantly enhanced capture efficiency as compared to flat surfaces.
Advanced Materials 05/2012; 24(20):2755. · 13.88 Impact Factor
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Nangang Zhang,
Yuliang Deng,
Qidong Tai,
Boran Cheng,
Libo Zhao,
Qinglin Shen,
Rongxiang He,
Longye Hong,
Wei Liu,
Shishang Guo,
Kan Liu,
Hisan-Rong Tseng,
Bin Xiong,
Xing-Zhong Zhao
[show abstract]
[hide abstract]
ABSTRACT: A nanostructured platform that combines electrospun TiO(2) nanofibers (TiNFs)-deposited substrate and cell-capture agent realizes significant capture of circulating tumor cells (CTCs). The enhanced local topographic interactions between the horizontally packed TiNFs deposited substrates and extracellular matrix scaffolds, in addition to anti-EpCAM/EpCAM biological recognition, contributes to the significantly enhanced capture efficiency compared to flat surfaces.
Advanced Materials 04/2012; 24(20):2756-60. · 13.88 Impact Factor
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Microfluidics and Nanofluidics 04/2012; · 3.37 Impact Factor
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[show abstract]
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ABSTRACT: In this paper, we demonstrate a new type of microfluidic chip that can realize continuous-flow purification of hydrogel beads from a carrier oil into aqueous solution by using a laminar-like oil/water interface. The microfluidic chip is composed by two functional components: (1) a flow-focusing bead generation module that can control size and shape of beads, (2) a bead extraction module capable of purifying hydrogel beads from oil into aqueous solution. This module is featured with large branch channels on one side and small ones on the opposite side. Water is continuously infused into the bead extraction module through the large branch channels, resulting in a laminar-like oil/water interface between the branch junctions. Simulation and experimental data show that the efficiency of oil depletion is determined by the relative flow rates between infused water and carrier oil. By using such a microfluidic device, viable cells (HCT116, colon cancer cell line) can be encapsulated in the hydrogel beads and purified into a cell culture media. Significantly improved cell viability was achieved compared to that observed by conventional bead purification approaches. This facile microfluidic chip could be a promising candidate for sample treatment in lab-on-a-chip applications.
Lab on a Chip 12/2011; 11(23):4117-21. · 5.67 Impact Factor
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Jing Sun,
Michael D Masterman-Smith,
Nicholas A Graham,
Jing Jiao,
Jack Mottahedeh,
Dan R Laks,
Minori Ohashi,
Jason DeJesus,
Ken-ichiro Kamei,
Ki-Bum Lee, [......],
R Michael van Dam,
Michael E Phelps,
Hong Wu,
Linda M Liau,
Paul S Mischel,
Jorge A Lazareff,
Harley I Kornblum,
William H Yong,
Thomas G Graeber,
Hsian-Rong Tseng
[show abstract]
[hide abstract]
ABSTRACT: The clinical practice of oncology is being transformed by molecular diagnostics that will enable predictive and personalized medicine. Current technologies for quantitation of the cancer proteome are either qualitative (e.g., immunohistochemistry) or require large sample sizes (e.g., flow cytometry). Here, we report a microfluidic platform-microfluidic image cytometry (MIC)-capable of quantitative, single-cell proteomic analysis of multiple signaling molecules using only 1,000 to 2,800 cells. Using cultured cell lines, we show simultaneous measurement of four critical signaling proteins (EGFR, PTEN, phospho-Akt, and phospho-S6) within the oncogenic phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway. To show the clinical application of the MIC platform to solid tumors, we analyzed a panel of 19 human brain tumor biopsies, including glioblastomas. Our MIC measurements were validated by clinical immunohistochemistry and confirmed the striking intertumoral and intratumoral heterogeneity characteristic of glioblastoma. To interpret the multiparameter, single-cell MIC measurements, we adapted bioinformatic methods including self-organizing maps that stratify patients into clusters that predict tumor progression and patient survival. Together with bioinformatic analysis, the MIC platform represents a robust, enabling in vitro molecular diagnostic technology for systems pathology analysis and personalized medicine.
Cancer Research 08/2010; 70(15):6128-38. · 7.86 Impact Factor
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Yanju Wang,
Wei-Yu Lin,
Kan Liu,
Rachel J Lin,
Matthias Selke,
Hartmuth C Kolb, Nangang Zhang,
Xing-Zhong Zhao,
Michael E Phelps,
Clifton K F Shen,
Kym F Faull,
Hsian-Rong Tseng
[show abstract]
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ABSTRACT: An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.
Lab on a Chip 09/2009; 9(16):2281-5. · 5.67 Impact Factor
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ABSTRACT: The microbes are intimately to our lives, therefore the researches of behaviors of microbes such as growth, division, anastomosis, transfection, transformation, death etc. are very important. Traditional methods such as freeze-etching method, can only find different growing periods through different cells so there is still no definite evidence for the existence of some biological activities. This would destroy a biological sample as well, so that later experiments can not be carried over smoothly. The effects of microscale heterogeneities cannot be replicated by conventional experimental techniques as well. We have recently incorporated PDMS-based valves and pumps for the analysis of single nonadherent yeast and bacteria cells. Optical trapping facilitated and ensured the encapsulation of single cells within the individual microdroplets. In this way, we have photographed the process of division of Saccharomyces sp. , and Bacillus megaterium.
Bioinformatics and Biomedical Engineering, 2007. ICBBE 2007. The 1st International Conference on; 08/2007
